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Biomedical subjects

M Katagiri

Publications and source records attributed to M Katagiri.

At least 199 records · Page 11Linked to original sources
PubMed1984

[Analysis of HLA-DR antigen expression on choriocarcinoma].

A choriocarcinoma and four choriocarcinoma cell lines were selected to investigate whether or not HLA-DR antigen is expressed on the cell surface of a choriocarcinoma which is regarded genetically as an allogeneic grafted tumor. Choriocarcinoma tissue was obtained from a uterus which was removed surgically, was prepared for immunoperoxidase staining method using monoclonal antibodies (7B6 and 8B3: They recognize monomorphic determinants on the HLA-DR antigen), and choriocarcinoma cell lines (BeWo, GCH, ENAMI and SCH) were prepared for inhibition test with radioimmunoassay using monoclonal antibodies (7B6 and Kop.). By immunoperoxidase staining, no HLA-DR positive choriocarcinoma cell (or myometrial cell) was demonstrated, although several cells of the uterine side, which contact the invasion of the choriocarcinoma showed positive staining. In an inhibition test, none of the four cell lines showed any inhibition. The present results suggested that HLA-DR antigen is not found in choriocarcinoma.

Adult↗
PubMed1983

Identification of proteins interacting with newly replicated DNA in SV40-infected cells by UV-induced DNA-protein cross-linking.

Protein species interacting with newly replicated DNA were analyzed using a photo cross-linking technique. Nascent DNA was labeled in vitro with [alpha-32P]dCTP and BrdUTP in SV40-infected CV-1 cells made permeable with saponin. The labeled cells were then irradiated with UV light (254 nm) and were treated extensively with DNase I. Proteins with radioactive DNA tags were separated by SDS-PAGE and visualized by autoradiography. Among 10-15 proteins which were cross-linked, the proteins with apparent molecular weights of 16.5 K, 44 K, 82 K and those in the 94-140 K region appeared to be associated with newly replicated SV40 DNA. A pulse-chase experiment showed that the 82 K and 94-140 K proteins interacted with new DNA in a relatively localized region close to the replication fork. The 44 K protein was identified as the major viral capsid protein, VP1, using antiserum to SV40 capsid proteins. It was suggested that VP1 binds to nascent DNA shortly after DNA synthesis and migrates into chromatin maturation regions.

Animals↗
PubMed1982

Association between HLA and Japanese patients with rheumatoid arthritis.

Japanese patients with rheumatoid arthritis (RA) were observed to have a statistical association with HLA-DR4, MT3. Strong association between the clinical severity of RA and HLA was also observed. Male patients had a stronger association with HLA than female patients. Males are more resistant to RA than females. This suggested that the threshold of liability for RA is higher in males than in females. Japanese patients with RA with systemic vasculitis were negative for HLA-Bw44 and had antilymphocytotoxic autoantibody, indicating that RA with systemic vasculitis is different in etiology from RA without systemic vasculitis.

Adult↗
PubMed1981

Mechanism of salicylate hydroxylase-catalyzed decarboxylation.

Salicylate hydroxylase (salicylate, NADH: oxygen oxidoreductase (1-hydroxylating, decarboxylating), EC 1.14.13.1) in Pseudomonas putida catalyzed hydroxylation of the substrate analogue, salicylaldehyde, to form catechol and formate with stoichiometric consumption of NADH and O2. Consequently, a study of primary product derived from the carboxyl group of the authentic substrate, salicylate, was undertaken. The experimental results revealed that CO2 not H2CO3, was produced first.

Aerobiosis↗