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Biomedical subjects

M Kaspar

Publications and source records attributed to M Kaspar.

25 records · Page 2Linked to original sources

[The morphological and functional integrity of cryo-preserved leukocytes].

Human peripheral blood lymphocytes were frozen by a simplified method which is described. Light and electron microscopic investigations of the frozen-thawed cells showed no significant morphological changes compared to the fresh cells; comparison of E-rosette formation and nonspecific acid esterase activity before and after cryoconservation afford evidence of a possible slight change in the percent relationship of T- and B-cells, whereas thymidine uptake values within normal range after stimulation with PHA and homologous cells demonstrate the functional integrity of the lymphocytes.

B-Lymphocytes↗

Thickness shear mode resonators ("mass-sensitive devices") in bioanalysis.

A short overview of function and experimental set-ups of acoustic wave devices is given which, in contrast to other bioanalysis techniques, are based on a mechanical transduction mechanism. The most frequently used device is the thickness shear mode resonator (TSMR), which in the last few years was intensively employed in biosensor applications. TSMR biosensor studies in the field of nucleic acid interaction, adsorption of proteins to surfaces and immunosensing are reviewed. A main point concerns the interpretation of the sensor response not only in terms of mass loading, which underestimates the capabilities of these devices.

Acoustics↗

Importance of digital subtraction angiography in the imaging of locomotor tumors.

Digital subtraction angiography (DSA) performed with modern sophisticated equipment has been improved substantially in its ability to document also small vessels and pathologic vascularization patterns in tumorous conditions of the locomotor apparatus. Its domain is in imaging vascular patterns hidden by structural (mainly sclerotic) shadows in their vicinity. In 152 patients out of the total patient collective in the Bone Tumor Register in Prague having a bone tumor or a tumor-like condition, DSA proved to be a mild method of choice for the diagnostic imaging of vascular patterns of the lesions and/or combined diapeutic interventions and has not lost its raison d'être in the era of CT and MRI.

Angiography, Digital Subtraction↗