The NAD(+)-specific glutamate dehydrogenase (NAD-GDH) of the filamentous fungus Neurospora crassa is a tetrameric enzyme, regulated by catabolite repression. The amino acid sequence of this enzyme had been published several years ago. With the object of investigating the molecular mechanism of catabolite repression, the nucleotide sequence of genomic clones containing the coding region, along with 5'- and 3'-flanking noncoding segments of the NAD-GDH transcription unit, was obtained. The gdh structural gene was shown to code for a polypeptide of 1047 residues, with a calculated molecular mass of 118,280 daltons. The coding sequence is interrupted by two short introns located close to the N- and C-terminal domains of the polypeptide. Consensus intron boundaries and internal splice sequences resemble closely those of other N. crassa genes. A comparison of the amino acid sequence deduced from the nucleotide sequence with the previously published sequence showed several discrepancies between the two. Nucleotide sequence corresponding to a gap in the amino acid sequence was located in the genomic clone. Genetic mapping by restriction fragment length polymorphism analysis localized the gdh gene close to the loci trp-1 and con-7 on the right arm of linkage group III.

Amino Acid Sequence↗

PubMedDate not supplied

Assessment of hospital services and patient reactions.

This paper deals with the study of assessment of hospital services and patient reactions carried out amongst 473 respondents, both in-door (3 wards) and out-door patients in M.L.B. Medical College Hospital, Jhansi (Uttar Pradesh). The patients were from three wards medical, surgical and gynaecological wards and consisted of 373 discharged and 100 out-door patients.

Consumer Behavior↗

PubMedDate not supplied

Congenital ocular anomalies in Pondicherry.

Congenital anomalies of the eye and ocular adnexa occurring in Pondicherry have been described. Anophthalmos, microphthalmos, limbal dermoids, nervus of Ota, anterior lenticonus, buphthalmos, and congenital cataract are very common. Onset of cataract in young individuals was caused by abnormal aminoaciduria. Hereditary factors played a part in 59 percent of the cases who would have become blind if they were not treated. Consanguinity is a major factor in their pathogenesis. Avitaminosis A and systemic infections which are common here may be playing a significant role in their etiology.

Adolescent↗

PubMedDate not supplied

A congenital corneal stromal cyst.

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Cataract↗