Search PubMed⌕ Search

Biomedical subjects

M Kano

Publications and source records attributed to M Kano.

At least 217 records · Page 12Linked to original sources
PubMed1987

Immunohistochemical demonstration of estrogen, progesterone, CEA and ferritin in breast cancer and their clinical value for the prediction of early postoperative recurrence.

The aim of this paper is to determine whether the incidence of estrogen-, progesterone-, CEA-, and ferritin- positive staining of primary tumors, by using the PAP method, is related to the prognosis of breast cancer status. A significantly higher incidence (71 per cent) of CEA-positive tumors was observed in patients who had a recurrence of breast cancer within 2 years after radical operation. Patients, whose tumors were positive in estrogen or negative in CEA, showed a relatively good prognosis, even after a recurrence of the disease. Distant metastases were seen in most of the patients positive in CEA (78 per cent). Before the recurrence of breast cancer, those patients positive in progesterone had a good prognosis. After the recurrence, however, there was no relationship between the prognosis of recurrent disease and the progesterone-staining of primary tumors. Our data suggest that the immunohistological staining of estrogen, progesterone and CEA might offer the effective prognostic indices in breast cancer patients.

Breast Neoplasms↗
PubMed1987

Granular cell tumor of the ascending colon: a case report.

A granular cell tumor (granular cell myoblastoma) of the ascending colon in a 48-year old male is reported. The tumor was detected by barium enema study as a sessile polyp, and colonofiberscopy revealed submucosal tumor. It was removed by endoscopic polypectomy. Macroscopic examination showed the characteristic features of granular cell tumor. The avidin-biotin-peroxidase complex (ABC) method for detection of S-100 protein demonstrated that the cytoplasm of tumor cells and the pleomorphic nuclei were strongly stained with anti-S-100 protein serum, which supports the concept of the Schwann cell origin of granular cell tumor.

Colonic Neoplasms↗
PubMed1987

Calcium channel components of action potential in chick skeletal muscle cells developing in culture.

The action potential was recorded from cultured chick skeletal muscle cells in Na-, Ca-, Cl-free saline containing Ba and tetraethylammonium ions (Ba saline). The action potential consisted of two components: a low-threshold, fast inactivating component and a high-threshold, long-lasting component. Both components of the action potential were dependent on external Ba ions and eliminated by Co ions. It is concluded that both components are generated by inward currents carried by Ba ions through Ca channels. The two Ca channel components of the action potential differed with regard to activation and inactivation potential, presence or absence of fast inactivation, sensitivity to an organic Ca channel blocker, and developmental profile. In addition, the failure of occurrence of one or the other components was observed in some cells. These results could be explained by assuming that two components of the action potential in Ba saline were mediated by the two different Ca channels. Furthermore, there was a tendency for younger cells to have more prominent Ca channel components. This may suggest that Ca channels have some function in the early stages of myogenesis.

Action Potentials↗
PubMed1986

Synaptic organization of the cerebello-thalamo-cerebral pathway in the cat. III. Cerebellar input to corticofugal neurons destined for different subcortical nuclei in areas 4 and 6.

To analyze the cerebellar effects on corticofugal neurons destined for different subcortical nuclei, intracellular recordings were made from corticofugal neurons in areas 4 and 6 of the cat. Corticonuclear neurons to the red nucleus (RN) and the pontine nucleus (PN), and pyramidal tract neurons (PTNs) with collaterals to these nuclei were identified by their antidromic responses to the stimulation of these nuclei and the pyramid. Three types of RN-projecting neurons (corticorubral neurons (CRNs), corticopontine neurons (CPNs) with a collateral to the RN and PTNs with a collateral to the RN) and two types of PN-projecting neurons (CPNs and PTNs with a collateral to the PN) were differentiated. Furthermore, these corticofugal neurons were classified as fast and slow neurons on the basis of a critical axonal conduction velocity of 20 m/s. About 80% of 98 RN-projecting neurons in area 4 were PTNs, and among the rest, CPNs were more common than CRNs. A similar tendency of the frequency distribution of 37 RN-projecting neurons was also observed in area 6. In area 4, about 70% of 158 PN-projecting neurons were PTNs (80 fast and 30 slow PTNs) and the rest were CPNs, while in area 6, only 35% of 99 PN-projecting neurons were PTNs (10 fast and 25 slow PTNs). Among the CPNs in areas 4 and 6, slow CPNs were more frequently encountered. Cerebellar effects on these identified corticofugal neurons were investigated, using electrical stimulation of the brachium conjunctivum (BC). In both areas 4 and 6, a substantial number of fast conducting CRNs, CPNs and PTNs projecting to the RN or the PN received short-latency (predominantly disynaptic), large-amplitude EPSPs from the BC, and a considerable number of slow conducting neurons to the RN and/or the PN received longer-latency, smaller-amplitude EPSPs from the BC.

Animals↗
PubMed1986

Piriform sinus fistula as a route of infection in acute suppurative thyroiditis.

A case of acute suppurative thyroiditis due to a left piriform sinus fistula was reported. A five-year old boy had a acutely painful tumor in the anterior part of the neck. It was a diffuse, firm, warm erythematous tumor, and laboratory, radiologic findings showed the acute inflammation of the left lobe of thyroid. a barium swallow revealed a very thin fistula originating from the apex of the left piriform sinus extending antero-inferiorly. This fistula was considered to be a route of infection in acute suppurative thyroiditis, allowing bacterial infection to begin in the perithyroidal space and spread to the thyroid gland. The complete fistulectomy was required for a permanent cure, but an administration of antibiotics was very effective in this case.

Bacterial Infections↗
PubMed1985

Acetylcholine receptors of human skeletal muscle: a species difference detected by snake neurotoxins.

The binding abilities of the nicotinic acetylcholine receptors (AChRs) of the skeletal muscles of man and other vertebrates to two typical curaremimetic toxins, erabutoxin b (Eb) and alpha-bungarotoxin (alpha-BT), were investigated. Fluorescent microscopy using rhodamine-labeled erabutoxin b (TMR-Eb) and FITC-labeled alpha-bungarotoxin (FITC-alpha-BT) revealed that AChRs of human and chimpanzee muscles were stained with FITC-alpha-BT, but not with TMR-Eb. In contrast, the AChRs of mouse muscle were stained with both fluorescent toxins. The stainings of human and chimpanzee AChRs with FITC-alpha-BT were inhibited by preincubation with unmodified alpha-BT, but not with either unmodified Eb or other short-chain neurotoxins. Binding experiments using 125I-labeled Eb ([125I]Eb) and 125I-labeled alpha-BT ([125I]alpha-BT) showed that the affinity of human AChRs for [125I]Eb was unusually low. Electrophysiological experiments showed that both acetylcholine potential and end-plate potential of human muscle were blocked by addition of alpha-BT, but not by Eb. On the contrary, acetylcholine potential of rat muscle was blocked by addition of Eb. All these results indicate that AChRs of human and chimpanzee muscles are different from those of other animals in having an exceptionally low affinity for Eb and other short-chain neurotoxins. The results suggest a heterogeneity among vertebrate AChRs concerning their reactivities to curaremimetic toxins.

Animals↗
PubMed1985

Long-term depression of parallel fibre synapses following stimulation of climbing fibres.

The results from several investigations suggest that climbing fibres heterosynaptically depress parallel fibre responses in Purkinje cells. In the present investigation the mechanism behind the depression has been studied by extracellular recording of responses in single Purkinje cells, evoked by electrical stimulation of parallel fibres and climbing fibres. The results show that a short time of conjunctive stimulation of climbing fibres and parallel fibres results in a long-lasting depression of the parallel fibre responses and that this depression can be prevented if the Purkinje cells are inhibited during the conjunctive stimulation. Since inhibition has been shown to shorten or abolish the long-lasting plateau potentials which are evoked by climbing fibre impulses this finding supports the assumption that the climbing fibre evoked plateau potentials mediate the heterosynaptic depression of parallel fibre responses.

Animals↗
PubMed1985

Synaptic organization of the cerebello-thalamo-cerebral pathway in the cat. I. Projection of individual cerebellar nuclei to single pyramidal tract neurons in areas 4 and 6.

The neural connections of the dentate (DN) and the interpositus (IN) nuclei to the motor cortex and area 6 were investigated by recording intracellular postsynaptic potentials from fast and slow pyramidal tract neurons (PTNs) in the anesthetized cat. Localized stimulation of DN and IN produced di- or polysynaptic EPSPs in fast and slow PTNs in the "forelimb area" of the motor cortex and area 6. The effects of stimulation of the two cerebellar projections were essentially the same, although some regional difference of their relative strength was noted. In these cortical areas, the majority of fast and slow PTNs received convergent inputs from both DN and IN. By examining the interaction of DN- and IN-evoked EPSPs, spatial facilitation and occlusion at the level of the thalamus were demonstrated. Therefore, it was concluded that at least a portion of the convergence of the dentate and the interpositus inputs occurred at the level of the ventrolateral nucleus of the thalamus.

Animals↗
PubMed1985

Synaptic organization of the cerebello-thalamo-cerebral pathway in the cat. II. Input-output organization of single thalamocortical neurons in the ventrolateral thalamus.

Input-output neural organization of single thalamocortical (T-C) neurons in the ventrolateral nucleus (VL) of the thalamus was investigated using an intracellular recording technique in the anesthetized cat. Stimulation of the dentate (DN) and the interpositus (IN) nuclei produced monosynaptic unitary EPSPs of large amplitude in T-C neurons projecting to the motor cortex or area 6 over the entire mediolateral region of VL. The thalamic projections from DN and IN are very wide and there is a considerable overlap between the dentate and the interpositus projection areas in VL. And in this overlapping area, a considerable number of T-C neurons (50%) receive inputs from both DN and IN. More than 40% of T-C neurons were antidromically activated from widely separated electrodes in the motor cortex, indicating that the cortical arbolization of single T-C neurons is very wide and the number of these neurons with widely divergent projections is considerably large.

Animals↗
PubMed1985

Cancer of the gallbladder in a 9-year-old girl.

Carcinoma of the gallbladder was found in a 9-year-old girl. The patient was transferred to our hospital because of an abdominal mass and right upper quadrant pain. X-ray cholecystography and ultrasonography were performed preoperatively. The ultrasonic scan showed a thickened wall and lack of contractility of the gallbladder. A laparotomy was performed under the diagnosis of chronic cholecystitis. At operation a peanut-sized nodule, in which adeno carcinoma was demonstrated by frozen section, was palpable at the neck of the gallbladder. Cholecystectomy was performed with T-tube drainage and postoperative chemotherapy was administered. The patient has had no evidence of tumour recurrence in 24 months of follow-up.

Adenocarcinoma↗
PubMed1985

Determination and significance of a new carbohydrate antigen CA19-9 in digestive system cancers.

To assess the diagnostic significance of CA19-9, the serum levels in 225 healthy subjects, 201 patients with cancers, 423 patients with benign diseases and 21 pregnant women, were determined by RIA. The mean CA19-9 level of the healthy subjects was 11.2 +/- 0.4 U/ml (range, 6-100 U/ml). Only 3.1% of them were above 37 U/ml. The CA19-9 levels were elevated above 37 U/ml in 7.9% of 293 patients with non-carcinomatous diseases of the digestive system. Among digestive system cancers, elevated levels were found in 18.2% of 11 patients with esophageal cancer, 42.7% of 68 patients with gastric cancer, 39.1% of 23 patients with colorectal cancer, 27.8% of 18 patients with primary hepatic cancer, 71.4% of 35 patients with biliary cancer, and 75% of 20 patients with pancreatic cancer. Most of the patients with levels above 100 U/ml had carcinomatous diseases. The CA19-9 positive rates for patients with gastric cancer and colorectal cancer were extremely low at stages I, II and III, while in patients at stage IV and in patients with recurrent cancer, a tendency for rapid increase in the positive rates and concentrations of CA19-9 was noted. Based on combination assay of CA19-9, CEA and ferritin, in comparison with the positive rates for CA19-9 alone, it was found that the rates were raised to 42.7% in gastric cancer, to 39.1% in colorectal cancer, and to 71.4% in biliary cancer, suggesting the simultaneous determination with these tumor markers may serve to elevate their usefulness.

Adolescent↗
PubMed1985

[Central alveolar hypoventilation as a complication of massive intracerebellar hemorrhage: report of a case treated by diaphragm pacing].

The author reported a case of central alveolar hypoventilation (Ondine's curse) which was treated by diaphragm pacing. A 59-year-old man was admitted because of sudden deep coma and tetraparesis. Neurological examination on admission showed miotic pupils with absent light reaction, no oculocephalic reflex, no corneal reflex and tetraparesis. Glasgow scale was evaluated to be E-1 V-1, and M-3 with total of 5 points. Computed tomography confirmed a large hematoma in the cerebellum with ventricular rupture. Immediately, a suboccipital craniectomy was performed and the hematoma was evacuated. His neurological condition was improved gradually within a month after the operation. He responded to verbal orders and moved all his limbs against gravity. His respiration, however, was irregular with a pattern of so-called "cluster breathing" followed by prolonged apnea, that was more pronounced in night. He was suffered from hypoxic hypercapnea and recurrent pneumonia. Therefore, authors decided to employ diaphragm pacing for management of central alveolar hypoventilation. A diaphragm pacemaker (radiofrequency induction) was implanted. A cuff electrode was put around the right phrenic nerve in the right thorax, and the receiver installed subcutaneously in the right anterior chest. Postoperative respiratory study showed ventilation on pacing with satisfactory blood gas and he became able to move around using a wheel chair. In 1966 Glenn demonstrated a new technique to move the diaphragm paced by a receiver through the phrenic nerve, triggered by radio wave from external device.(ABSTRACT TRUNCATED AT 250 WORDS)

Cerebellar Diseases↗
PubMed1984

Immunosuppressive activity of sera from gastric cancer patients.

Sera from 60 gastric cancer patients and 20 patients with benign gastric diseases and 8 healthy controls were tested for inhibitory effects on the humoral response to sheep erythrocytes (SRBC) by the plaque forming cell assay (PFC R.I.) using mouse spleen cells and on the phytohemagglutinin (PHA)-induced blastogenesis of normal mouse spleen cells (PHA S.R.). Gastric cancer patient sera showed a significantly lower PFC R.I. than did sera from benign gastric disease patients and from the healthy controls. However, there was no appreciable interstage difference in the degree of depression. The PHA-induced blastogenesis of normal spleen cells was also decreased in the presence of sera from cancer patients, as compared to that in the presence of sera from benign disease patients and from the healthy controls. The depression progressed with advancing stage of cancer. The PHA S.R. showed significant negative correlations with serum levels of IAP, IS, alpha 1-acid glycoprotein and alpha 1-antitrypsin, but there were no such correlations between PFC R.I. and these glycoproteins in serum. There was also no correlation between the values of the PHA S.R. and the PFC R.I. These results suggest that these two assays may depict immunosuppressive activities operating through entirely different mechanisms.

Animals↗