Gas CT cisternography of trigeminal neuralgia caused by AVM of the cerebellopontine angle.
Explore the source record for details and available documents.
Cerebellopontine Angle↗
Biomedical subjects
M Kabuto
Publications and source records attributed to M Kabuto.
Captopril inhibits glioma cell invasion in vitro: involvement of matrix metalloproteinases.
We investigated the effects of captopril on the activity of matrix metalloproteinases (MMPs) secreted by T98G glioma cells and their invasiveness in vitro. On gelatin zymography, captopril inhibited gelatinolytic activities in the culture media of T98G cells. This inhibitory effect was reversed by the presence of excess zinc. In an in vitro invasion assay, invasion into the reconstituted basement membrane (Matrigel) by T98G glioma cells was inhibited by captopril in a dose-dependent manner. This inhibitory effect was also reversed by the addition of zinc to the culture media. However, at the effective concentration of captopril for the prevention of tumor cell invasion it did not inhibit the motility, adhesion to Matrigel or proliferation of T98G cells. These findings suggest that captopril inhibits the invasiveness of T98G glioma cells due to its MMP inhibitory activity, chelating zinc ions at the active center of MMPs.
Angiotensin-Converting Enzyme Inhibitors↗
Hyaluronic acid facilitates glioma cell invasion in vitro.
We investigated the effects of hyaluronic acid (HA) on the invasiveness of three human glioma cell lines (A172, T98G and U251) and their secretion of matrix metalloproteinases (MMPs), plasminogen activators (PAs), and hyaluronidase. The invasion of all three glioma cell lines was enhanced by the impregnation of Matrigel with HA in an in vitro invasion assay using 12 microns porosity polycarbonate filter transwells. The secretion of MMPs and PAs was not influenced by the presence of HA. Hyaluronidase activity was not detected in the culture media of any glioma cell line. HA also enhanced the motility of A172 and U251 glioma cells, but did not influence the motility of T98G glioma cells. The adhesion and spreading of all glioma cell lines were inhibited on HA-coated plates. HA, however, did not influence the proliferation of any of the glioma cell line. These results suggest that the presence of HA contributes to glioma cell invasion, which involves the stimulation of detachment and motility of glioma cells and the maintenance of proteinase secretion by glioma cells.
Cell Adhesion↗