Search PubMed⌕ Search

Biomedical subjects

M K Gupta

Publications and source records attributed to M K Gupta.

At least 73 records · Page 4Linked to original sources
PubMed1989

The role of the reticuloendothelial system in the immunopathology of Marek's disease.

The role of macrophages in immunity against Marek's disease (MD) was studied. Chickens of one group were subjected to depletion of macrophages using repeated doses of Francil amorphous silica and those of another group were subjected to activation of macrophages using repeated doses of brewer's thioglycollate broth. Chickens of a third group were vaccinated with herpesvirus of turkeys FC 126 vaccine followed by depletion of macrophages. Chickens of these three groups, as well as groups of healthy unvaccinated and healthy vaccinated chickens, were challenged with virulent MD virus. A sixth group of healthy uninfected chickens was kept as a control. The results, based on clinical signs, gross and histopathological studies and agar gel precipitation test (AGPT) for antibodies, indicated that activation of macrophages enhanced immunity against MD and depletion of macrophages had the opposite effect. The protective effect of vaccination against MD was also lowered by depletion of macrophages. The results of AGPT indicated retardation of MD virus replication by macrophage activation and the reverse on depletion.

Animals↗
PubMed1989

Lipid profile in smokers.

In this study we observed that all the lipids except HDL cholesterol were higher in heavy smokers and those making for longer duration. HDL cholesterol was lower in smokers than nonsmokers. The changes in lipid profile were similar in cigarette and bidi smokers.

Adult↗
PubMed1988

Measurement of immunoreactive myelin basic protein peptide (45-89) in cerebrospinal fluid.

To measure myelin basic protein (MBP)-like material in cerebrospinal fluid, we compared two radioimmunoassays, both using the same antiserum to MBP but one using peptide (45-89) as the radioligand and standard (peptide assay), and the other using purified MBP as the radioligand and standard (MBP assay), with respect to their diagnostic sensitivity. Cerebrospinal fluid specimens from 185 patients with definite multiple sclerosis (MS) (n = 27), possible MS (n = 63), probable MS (n = 24), and other neurological disease (n = 71) were analyzed using both assays. The diagnostic sensitivity of the peptide assay was significantly better than that of the MBP assay in those with definite MS (sensitivity 59% and 30%, respectively); there was no significant difference in specificity. The peptide assay also showed better correlation with disease activity than the MBP assay: 14 patients classified as having active MS showed significantly higher sensitivity (78.6% versus 38%, p less than 0.04) when compared to patients with inactive disease. The MBP assay showed no significant difference between these two groups. Besides the increase in sensitivity, the actual molar concentrations of immunoreactive MBP detected using this peptide assay were considerably higher than those found using the MBP assay. These results show that the use of MBP antisera capable of recognizing epitopes present in the carboxyl half of MBP peptide (45-89) results in more sensitive detection of immunoreactive MBP when used with MBP peptide (45-89) as radiolabeled ligand in the assay.

Humans↗
PubMed1988

In vivo immunosuppression by synthetic pyrethroid (cypermethrin) pesticide in mice and goats.

Cypermethrin toxicity was produced in mice by intra-peritoneal injection of the pesticide at 50 mg/kg body weight per day for 26 days, and in goats by drenching with cypermethrin at 41.6 mg/kg body weight per day for 30 days. The status of cell-mediated immunity (CMI) was assessed by the 2,4-dinitrofluorobenzene (DNFB) skin sensitivity test. The results indicated significant depression of CMI in the cypermethrin-treated mice and goats. In addition, the humoral immune reaction of the goats intoxicated with cypermethrin was estimated by enumeration of the plaque-forming B-lymphocytes. The rate of plaque formation in the lymphocyte suspension of cypermethrin-treated goats was significantly reduced and the diameter of the plaques was also significantly lower than in that of control animals. The results indicated that cypermethrin suppressed both CMI and the antibody-forming ability of lymphocytes. This immunosuppressive action of cypermethrin is being reported for the first time.

Animals↗
PubMed1988

Steroid receptors in human brain and spinal cord tumors.

Previous investigators have shown evidence of hormonal receptor protein in human brain tumors. In spite of conflicting results, antiestrogen agents (e.g., tamoxifen) have been used in clinical trials of recurrent unresectable meningiomas. In an effort to accrue further comprehensive in vitro data on this subject, we have evaluated 50 human brain and spinal tumors for estrogen, progesterone, and androgen receptor markers. Twenty-nine of the 50 tumors were meningiomas. The other 21 included 11 gliomas of various grades, 5 schwannomas, 3 metastatic carcinomas, 1 angiofibroma, and 1 craniopharyngioma. Only 8 tumors, all meningiomas, were positive for both progesterone and androgen receptors. The 8th tumor was positive for all three receptor proteins. Our study did not find a significant relationship between meningiomas and the presence of steroid receptor protein. We conclude that the use of antiestrogen agents is not indicated in the treatment of meningioma. No significant relationship to sex, menopausal status, tumor type, or tumor location was observed.

Brain Neoplasms↗
PubMed1988

Recent advances in laboratory tests for autoantibodies to thyrotropin receptor protein in Graves' disease.

Graves' disease is characterized by the presence of autoantibodies with variable reactivities to TSH receptor protein. These autoantibodies are heterogeneous in nature and may act as TSH agonists or infrequently as TSH antagonists (blocking antibodies). With new technical advances, it has become possible to quantitate these antibodies and their reactivities in clinical laboratories. Technical aspects of these assays and their clinical utility in the diagnosis and management of patients with Graves' disease are discussed.

Autoantibodies↗
PubMed1987

Myelin basic protein and demyelinating diseases.

Myelin basic protein (MBP) is a major protein component of myelin sheath. Primarily because of its ability to induce experimental allergic encephalomyelitis (EAE) in animals, this protein has been considered to play an important role in the pathogenesis of multiple sclerosis (MS), which is one of the most common demyelinating diseases. Its precise measurement in the cerebrospinal fluid (CSF) has been uncommonly difficult, mainly due to immunoheterogeneity of MBP or MBP-like material. More recently, highly sensitive radioimmunoassay techniques utilizing well-characterized antisera have been developed that facilitated its use in the management of MS. The clinical course of MS is highly variable, and the disease is characterized by periods of remission and relapses. Many studies have demonstrated the release of MBP during relapses and elevated levels of MBP in the CSF can be detected if lumbar puncture is performed within 7 days of the onset of neurologic symptoms suggestive of MS. However, the presence of MBP is not an absolute indicator of MS, as elevated MBP levels are also frequently observed in other demyelinating diseases.

Antigen-Antibody Complex↗
PubMed1987

Volume correction factor in time dose relationships in brachytherapy.

Paterson's clinical data about the maximum tolerance doses for various volumes of interstitial implants with Ra-226 delivered in seven days was made use of in deriving volume correction factors phi v and phi'v for TDF and CRE concepts respectively for brachytherapy. The derived volume correction factors phi v for TDF and phi'v for CRE differ from the from the one assumed for CRE by Kirk et al. and implied for TDF by Goitein. A normalising volume of 70 cc has been suggested for both CRE and TDF concepts for brachytherapy. A table showing TDFv, the volume corrected TDF, is presented for various volumes and dose rates for continuous irradiation. The use of this table is illustrated with examples.

Brachytherapy↗
PubMed1986

Odontoameloblastoma.

A case of odontoameloblastoma affecting the left mandible is reported. Initially treated by conservative excision due to its small size, the lesion recurred after 18 months and was treated by radical excision and subsequent reconstruction by a rib graft.

Ameloblastoma↗
PubMed1986

Osteomyelitis of the mandible in marble bone disease.

Marble bone disease is a rare skeletal disorder. Osteomyelitic changes in the jaw bones are frequently seen in this disease. Considering the rarity of the disease, a case of osteomyelitis of the mandible in marble bone disease is presented, together with a review of the available literature.

Child↗
PubMed1986

Mandibular osteomyelitis caused by Actinomyces Israelii. Report of a case.

An unusual case of cervicofacial actinomycosis with cortical osteomyelitis of the ascending ramus is presented. The portal of entry of the infection was most probably by an extra oral route. The authors have emphasized the importance of repeated smear examinations, cultures, and sensitivity tests to rule out actinomycosis in such cervicofacial infections. The difficulties encountered in the isolation of A. Israelii are discussed.

Actinomyces↗