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Biomedical subjects

M K Bhatnagar

Publications and source records attributed to M K Bhatnagar.

52 records · Page 3Linked to original sources
PubMed1975

A possible autoimmune parathyroiditis following ozone inhalation. II. A histopathologic, ultrastructural, and immunofluorescent study.

Histologic, ultrastructural, and immunofluorescent changes in the parathyroid glands of rabbits have been studied after 48 hours of ozone inhalation at a dosage of 0.75 ppm. The frequent changes observed included hyperplastic parathyroiditis followed by capillary proliferation and leukocytic infiltration. The progressive cytologic events consisted of the presence of eosinophilic leukocytes, reticuloendothelial and lymphocytic infiltration, disaggregation of the parenchyma, and interstitial edema. The ultrastructural changes consisted of degeneration of nuclei, atrophy of the mitochondria, dilatation and atrophy of the endoplasmic reticulum of the chief cells of the parathyroid gland, proliferation of the venous limb of the capillary network, and the prominent interstitial elements. The immunofluorescent techniques revealed positive immunologic response. These data suggest that ozone inhalation perhaps triggers an immune reaction which causes inflammatory injury to the parathyroid gland. The possibility that the modified functional chemical groups of the parathyroid gland act as autoantigen is discussed.

Aerosols↗
PubMed1975

Fatty haemorrhagic liver syndrome in laying hens on diets supplemented with rapeseed products.

Livers of laying hens of Hy-Line No 934E on low erucic acid rapeseed meals and rapeseed oil were studied. Gross lesions in the livers of hens on experimental diets were moderate to severe fatty degeneration, focal necrosis and moderate to severe haemorrhage. Histological examination revealed oedematous foci and lysis of hepatocytes along with large amounts of lipid droplets in the necrotic lesions. Necrotic lesions were not always associated with large haemorrhages. Connective tissue infiltration of older degenerative and haemorrhagic lesions was not extensive. Abdominal haemorrhage from livers occurred when extensive necrosis in the form of hepatocyte lysis and some vascular changes were present, suggesting hepatocytic degeneration caused by toxic products or their metabolites present in rapeseed by-products.

Animals↗
PubMedDate not supplied

Ethanol potentiation of methyl mercury toxicity: a preliminary report.

The ability of ethanol to affect hindlimb ataxia and body weight changes induced by methyl mercury was studied in rats. Animals treated with either water or ethanol increased in body weight during the experiment and showed no impairment of hindlimb movement. Rats treated with methyl mercury also increased in body weight but developed moderate hindlimb ataxia. Animals treated with ethanol and methyl mercury initially gained but subsequently lost weight and exhibited severe hindlimb ataxia. The results provide evidence that ethanol can potentiate methyl mercury toxicity in rats and, by implication, in humans.

Animals↗
PubMedDate not supplied

Self-collection by diabetic patients of capillary blood for free insulin monitoring; reduction by diamide of haemolysis-induced insulin loss.

The need to precipitate bound insulin immediately after withdrawal of blood and the tendency to haemolysis, which reduces immunoassayable insulin, have prevented development of methods of self-collection of capillary blood for later free insulin measurement. We therefore investigated the use of the thiol-oxidizing agent, diamide, to prevent insulin loss with haemolysis and developed a self-collection procedure with capillary tubes pre-filled with diamide and polyethyleneglycol (PEG, for separation of free and bound insulin). Diamide (final concentration 5 mmol l-1) reduced serum insulin loss from 48 +/- 4 (+/- SE) to 11 +/- 4% (p less than 0.001) in maximally-haemolysed samples. The effect of diamide was concentration-dependent up to 5 mmol l-1. Diamide had no effect on the standard curve for radioimmunoassay of insulin. Levels of serum free insulin in self-collected capillary blood were significantly correlated with venous serum free insulin in 22 non-diabetic subjects (r = 0.92, p less than 0.001), 52 Type 1 diabetic patients (r = 0.86, p less than 0.001), and 18 Type 2 diabetic patients (r = 0.97, p less than 0.001). Mean capillary free insulin concentration was higher than in venous serum (22% in normal subjects, 64% in Type 1, and 23% in Type 2 diabetic patients). Storage at room temperature of capillary blood containing PEG/diamide for 72 h did not alter immunoassayable insulin concentrations.

Blood Specimen Collection↗
PubMedDate not supplied

Laparoscopic spinal fusion.

We have reviewed the history of laparoscopic fusions and our results over the past two years, utilizing a femoral ring allograft and bone graft in 51 patients. The rate of fusion in this series is nearly 100%, with relief of radicular pain in about 90%. The average hospital stay is approximately two days and return to work is about six weeks. Minimally invasive techniques for spinal surgery will evolve with time and will become the standard of care in the near future.

Diskectomy↗
PubMedDate not supplied

Cartilage lesions associated with shaky-leg lameness in turkeys.

Shaky-leg lameness is a poorly defined, mild to very severe, painful lameness occurring mainly in 10-to-18-week-old fast-growing male turkeys. Since there are no significant gross or microscopic lesions in early or uncomplicated cases, the cause of the lameness is likely soft-tissue (muscle or tendon) pain. A case history of a flock problem of shaky-leg lameness is described, and it is suggested that stiffness and inactivity secondary to footpad dermatitis in these turkeys was responsible for the severe shaky-leg lameness in the flock. The cartilage lesion described is an avascular lesion and appeared to be the result of inactivity rather than the cause of lameness in these turkeys.

Age Factors↗
PubMedDate not supplied

Ultrastructure of the thyroid glands of rats fed photomirex: a 48-week recovery study.

The thyroid glands of rats that received photomirex (0.05, 0.5, 5 or 50 ppm) or mirex (5 or 50 ppm) in the diet for 28 days and were then kept on clean feed for 48 weeks were examined by an electron microscope. A dose of 0.05 or 0.5 ppm photomirex produced cytoplasmic vacuolation in the principal follicular cells; the cells were taller than the cuboidal follicular cells found in the glands of animals from the control group. An extensive accumulation of deformed lysosomal bodies, a reduction in the number of apical vesicles and a mild dilatation of rough-surfaced endoplasmic reticulum (RER) cisternae were the features of columnar follicular cells at the 5 ppm photomirex level. In the animals receiving 50 ppm photomirex the follicular cells had more severe alterations, including a marked dilatation of RER profiles, compared to those in the preceding groups. The cells from rats ingesting 5 ppm mirex were least affected and had only a mild dilatation of RER elements, and some cytoplasmic vacuolation. The follicular cells of animals fed 50 ppm mirex showed variable morphology; some of these cells had an increased number of colloid droplets and lacked the lamellar arrays of RER; others contained vacuoles or somewhat dilated RER profiles. The present results indicate that following the ingestion of photomirex (0.05-50 ppm) or mirex (50 ppm) for 28 days, alterations in the rat thyroids persist for at least one year.

Animals↗