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Biomedical subjects

M Just

Publications and source records attributed to M Just.

At least 181 records · Page 10Linked to original sources
PubMed1975

Is there any transtubular reabsorption of filtered proteins in rat kidney?

The bovine protease inhibitor aprotinin (Trasylol) has a high affinity to the kidney and is preferentially pinocytized in the proximal tubule. After i.v. injection of 1mug 124 I aprotinin the blood content decreases to 2.8% of the primary injected amount within 3 hrs, while simultaneously each kidney contains 29%. This substance was used to test whether or not a peptide which is pinocytized, is released in the intact form into the peritubular blood. By a cross circulation technique with two unilaterally nephrectomized rats we were unable to detect any transport of pinocytized, intact peptide through the proximal tubule cell over the observed cross circulation period of 1-8 hrs even when using 5000 times the above dosage. Since the total amount of aprotinin in the kidney is immunologically reactive (ca. 97%), and 65% of the radioactivity in the blood is not reactive after 6 hrs, we believe that the last step in the absorption process consists in digestion inside the lysosomes and instantaneous release of the split products into the blood.

Animals↗
PubMed1975

In vivo interaction of the Kunitz protease inhibitor and of insulin with subcellular structures from rat renal cortex.

Rats were injected with labeled Kunitz protease inhibitor and killed at various times thereafter. Radioactivity was measured in various fractions of kidney homogenates in order to study the time-dependent fixation to different cell organelles, expecially the transition from the brush border to lysosome fraction. With short survival periods (up to 5 min), the renal protease inhibitor is recovered nearly completely with the brush border fraction. With longer periods, a shift towards particles with higher densities and higher beta-glucuronidase activities takes place. Similar results have been achieved with insulin. Lysosomes were prepared and subfractionated following i. v. administration of the protease inhibitor or insulin. The radioactivity of the peptides was found in the lysosomal range of density. According to our present and previous results, the renal pathway of the protease inhibitor consists of 3 steps: binding to the brush border, reabsorption into micropinocytotic vesicles and phagosomes, and final enrichment in phagolysosomes with subsequent degradation. We suggest this type of transport to be representative for peptides in general.

Animals↗
PubMed1975

Immunisation trials with live attenuated cytomegalovirus TOWNE 125.

Trials with live cytomegalovirus (TOWNE 125 strain), which was attenuated by 125 passages exclusively on WI-38 cells, were done in adult volunteers. No virus take occured after oral/nasal application. When 10(3) TCD50 was given intramuscularly an IgG antibody response was detected at four weeks in all of ten volunteers tested by immunofluorescence; an IgM response was found in seven. Only mild local side-effects and relative lymphocytosis were observed. No virus excretion was found. Many questions remain to be answered by further trials before a cytomegalovirus vaccine can be given to adolescent girls.

Antibodies, Viral↗
PubMed1975

The renal handling of biologically active peptides.

With the use of the protease inhibitor from bovine organs--Trasylol-- as a model, we studied the pinocytic transport of peptides in the kidney. Rats were injected with the 125I-labeled peptide and killed at different times thereafter. Kidney homogenates were subfractionated by differential and sucrose gradient centrifugation. Radioactivity was measured in the fractions in order to study the time-dependent fixation of the peptide to different cell organelles. With short survival periods, the protease inhibitor is recovered in the brush-border fraction, with longer periods, a shift towards the lysosome fraction takes place. Thus, the renal transport of the protease inhibitor consists of three steps: binding to the brush border, incorporation in micropinocytic vesicles and transport in phagolysosomes.

Animals↗
PubMed1975

Human interferon therapy for herpes zoster in adults.

Of 37 patients with herpes zoster 28 were treated with human exogenous interferon and 9 received only culture medium. The interferon was produced in leukocyte cultures and was given intramuscularly in one daily dose of 1 million units for 5-8 days. In the interferon-treated patients interferon was detectable in serum (peak level 1-5 hours after interferon injections) and in vesicle fluid, and in some patients also in urine samples. Anti-interferon antibodies were not found. Of the 28 interferon-treated patients 8 had a slight temperature increase and 4 showed a transient local reaction. In the interferon treated group of patients the duration of pain was shortened and the development of crust formation was enhanced compared with the control group.

Adolescent↗
PubMed1975

[Interpretation of orthodontically treated by means of punched cards. 2. Data at the completion of treatment].

The authors analysed the key-word cards of 3245 terminated orthodontic cases. Statistical data were obtained about treatment results, therapeutical aids, duration of treatment and co-operation of the patients. These data give a survey on the achievements of the orthodontic practitioner, the choice of his methods and the utilization of his therapeutical aids. The authors could draw certain conclusions as to their own work in practice. Furthermore, they realized that the use of key-word cards permits to evaluate critically their achievements and to perform collective scientific studies.

Dental Records↗
PubMed1975

Circulating interferon in man after administration of exogenous human leukocyte interferon.

Interferon was measured at different intervals after the iv, sc, and im application of exogenous human leukocyte interferon to patients with various virus diseases or neoplasms. Interferon injected iv into patients had a half-life of about 15 minutes in the 1st hour and of about 90 minutes in the next 3 hours. Six hours after iv injection of 30 million U, no serum interferon was detectable. With a continuous iv infusion, a relatively high serum interferon level was reached. By the im administration of 1 million U interferon, a peak level of serum interferon (mean value 107 U/ml serum) occurred after 2 hours and was fairly stable for about 6 hours. Twenty-four hours after im application, a low level of serum interferon was still detectable. Similar results were found after sc interferon injections. In a patient with subacute sclerosing panencephalitis, no interferon was found in the cerebrospinal fluid at the time of the highest serum interferon level and 24 hours after two im interferon injections. Only minimal side reactions resulted from sc and im interferon injections. In one patient, a shock reaction occurred after iv application. For therapeutic trials, about 1 million U exogenous human interferon should be injected twice daily im or sc.

Encephalitis↗
PubMed1975

[The distribution of ampicillin between blood and cerebrospinal fluid in patients with and without serous meningitis (author's transl)].

1. With a constant plasma concentration of Ampicillin the ratio of concentration in the cerebrospinal fluid to concentration in the plasma reaches a constant value after 2 hrs in patients with and without serous meningitis. 2. Patients without serous meningitis have a value of 0.025, patients with serous meningitis a value of 0.061. 3. From this it can be calculated that Ampicillin passes two and a half times faster through inflamed meningeal membranes than unaffected membranes.

Ampicillin↗
PubMed1975

[Blood-liquor distribution of ampicillin in children with meningitis and without (author's transl)].

The distribution of ampicillin between blood and cerebrospinal fluid was compared in patients with serous meningitis to that in patients without inflammation of the meningeal membranes. Ampicillin was applied as a continuous intravenous infusion. Samples of blood and cerebrospinal fluid were taken after 1,2,4 or 12 h for the determination of the ampicillin concentrations. In both groups of patients the ratio of concentration in cerebrospinal fluid to concentration in plasma rose between the first and second hour after administration and reached a steady-state value thereafter. This steady-state ratio was 0.025 in patients without meningitis and 0.061 in patients with meningitis. These results are discussed in the context of a pharmacokinetic model which describes the distribution of drugs between blood and cerebrospinal fluid.

Ampicillin↗