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Biomedical subjects

M Jurin

Publications and source records attributed to M Jurin.

At least 37 records · Page 2Linked to original sources
PubMed1982

Influence of sera from tumor bearing mice on immune reaction.

Immunopotentiation abilities of sera from mice bearing fibrosarcoma or lymphoma for various periods of time were assayed using the plaque forming cell (PFC) technique. The influence of such sera on cell proliferation, evaluated by the spleen index, was determined in lethally irradiated mice reconstituted with syngeneic bone marrow cells. Sera from tumorous animals stimulated the number of PFC significantly, fibrosarcoma sera being more effective in the advanced stage and lymphoma in the initial phase. Sera from mice with both tumors in the early phase increased spleen indices significantly, and sera from mice with advanced tumors were less effective. The possible role of lymphokines in these events is discussed.

Animals↗
PubMed1977

[Morphological and cytochemical investigation of Ehrlich ascites tumor cells of the solid form as compared with peritoneal exudate in mice (author's transl)].

Ehrlich ascites tumor cells in peritoneal exudate of mice show a more regular round shape, greater and multiple nucleoli and more abundant and dark cytoplasma in comparison with cells of the same tumor transplanted in the limb. In solid tumor cells activity of naphthol-AS-acetate esterase is significantly higher than in exudate cells which demonstrate higher activity of PAS reaction. Other cytochemical reactions show no significant differences between the two cell forms. It seems that exudate cells are biologically more active than cells from the solid tumor.

Animals↗
PubMed1977

In vitro suppression of macrophage spreading caused by supernatants of tumour, thymus, and lymph node cells.

Peritoneal macrophages washed out from C3Hf/Bu mice were cultivated in medium 199 supplemented with 35% foetal calf serum. If the pH of the medium was adjusted to 7.2, there were 70% spread macrophages after six hours of incubation at 37 degrees C. We investigated the effect of tumour cells on macrophage spreading. Supernatants from cell cultures of a fibro-sarcoma of C57BL mice and lymphoma of C3Hf/Bu mice were used to determine the number of spread macrophages after 6 hours of culture in these supernatants. Supernatants from cell cultures of the kidney, thymus and lymph nodes of normal C57BL or C3Hf/Bu mice and peritoneal cells of normal C3Hf/Bu mice in medium 199 alone served as control. We observed that supernatants from both allogeneic and syngeneic tumour cell cultures reduced significantly the percentage of spread macrophages in comparison with the percentage of spread macrophages found in the kidney culture supernatants. However, a similar spreading inhibition was caused also by supernatants from the thymus and lymph node cell cultures. In these experiments we confirmed the earlier observations that tumour cells can suppress the activation of macrophages, and we also pointed to the possibility that this effect is not specific only for malignant tissues.

Animals↗
PubMed1974

Natural history of mouse syngeneic lymphoma. Morphologic and immunologic aspects.

The morphologic changes in lymphoreticular tissues and development of antitumor immune reactions of specific pathogen-free mice injected with syngeneic lymphoma cells were sequentially analyzed. The regional (right inguinal) lymph node demonstrated mild changes indicative of immunologic response. Systemic lymph nodes revealed a moderate degree of immune response on morphologic basis. The spleen was the site of marked activity, characterized by the presence of large pyroninophilic cells and germinal centers. Foci of necrosis in the local tumor accompanied by mature lymphocytes suggested cell-mediated immune rejection. Mice developed circulating antibodies 2 days after implantation. No antibodies were demonstrated attached to fresh tumor cells. Lymphocyte cytotoxic activity was demonstrated beginning on day 4. Both cytotoxic activity and circulating antibodies were no longer detectable after the third week following tumor implantation. Tumor-bearing mice also had an impaired capacity to mount a primary immune reaction to sheep red blood cells. The spleen demonstrated a marked loss of lymphocytes and the subsequent appearance of masses of amyloid material. It is suggested that amyloidosis in lymphoreticular organs is the result of a derangement in the immune response of the host following a prolonged and sustained antigenic stimulation. It appears that in syngeneic pathogen-free mice the spleen plays the major role in immune rejection mechanisms while the draining node only plays a modest role.

Amyloid↗
PubMedDate not supplied

Antitumorous and immunomodulatory effects of the Viscum album L. preparation Isorel.

There are numerous data on the immunostimulative and antitumorous activity of various Viscum album tissue extracts. Isorel (Novipharm, Austria) is one of these compounds. We found that in mice an increased number of plaque-forming cells to sheep red blood cells (SRBC) followed the injection of Isorel together with SRBC. Further, survival time of a foreign skin graft was shortened if Isorel was applied at the correct time. Finally, suppressed immune reactivity in tumorous mice recovered following Isorel injection. Isorel was further shown to be cytotoxic to tumor cells in vitro. Its application to tumor-bearing mice could prolong their life but without any therapeutic effect. However, a combination of local irradiation and Isorel was very effective: following 43 Gy of local irradiation to a transplanted methylcholanthrene-induced fibrosarcoma (volume about 240 mm3) growing in syngeneic CBA/HZgr mice, the tumor disappeared in about 25% of the animals; the addition of Isorel increased the incidence of cured animals to over 65%. The combined action of Isorel, influencing tumor viability on the one hand and the host's immune reactivity on the other, seems to be favorable for its antitumor action in vivo.

Animals↗
PubMedDate not supplied

[Inhibition of growth of B16 melanoma caused by liver regeneration].

Phenomenon of spontaneous regression of cancer indicates that recovery from malignant disease can happen without the application of any so far known therapeutic treatment. The most of the self-cured patients have undergone surgical operation which did not eliminate entire tumor or did not affect malignant tissue at all. Furthermore, regression or reversion of tumors (transformation of tumor cells into normal, nonmalignant cells) can be achieved in plants or amphibia after exposure of tumor cells to the influence of normal, regenerating tissue. Thus, it seems that during tissue regeneration certain local changes in tissue happen (synthesis of some regulatory growth factors) which induce dying of tumor cells or modify main features of malignant cells. Previously we have studied growth of murine malignant tumors in regenerating tissue of liver and skin. Obtained results indicated that under the influence of regenerating tissue anaplasia of fibrosarcoma decreases, as well as do pigmentation and the incidence of live cells in melanoma B16 tissue. Thus, it is obvious that mechanism of regenerating tissue growth control, which can also change characteristics of tumor cells, exists in mammalia, too. In order to analyse whether the same homeostatic mechanism is responsible for the phenomenon of spontaneous regression of human cancer, we have analysed the growth of melanoma B16 in back limb of nonoperated and sham or partially hepatectomized mice. Regeneration of skin and abdominal wall tissue in sham hepatectomized animals slowed down tumor growth, while liver regeneration completely inhibited tumor progression. Tumor growth inhibition was result of tumor tissue necrosis which developed around blood vessels. However, the structure and integrity of blood vessels themselves was normal.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMedDate not supplied

[The value of the lymphocyte transformation index induced by mitogens in cervical carcinoma].

Dynamics of immune reactivity in patients with the carcinoma of the cervix was determined. The transformation of their lymphocytes was induced in vitro by phytohemagglutinin (PHA), and the transformation index--a correlate of immunological reactivity--was determined at the following intervals: just before the start of the therapeutic procedure, at the end of the procedure, and every three months afterwards. Very low values of the transformation index, i. e. pronounced immunosuppression, characterized the initial period in all the patients, regardless of either the degree of the disease or the age of the patients. The suppression was prominent during 6 to 18 months following the therapeutic procedures and was overcome earlier in young patients bearing smaller tumors. The results indicate that in tumor-bearing patients a pronounced suppression of immune reactivity occurs after the PHA administration and that it could persist several months after the therapy.

Female↗