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Biomedical subjects

M Jarosz

Publications and source records attributed to M Jarosz.

At least 19 recordsLinked to original sources
PubMed2001

Atmospheric pressure chemical ionization mass spectrometric and visible spectrophotometric studies of copper(I) and copper(II) complexes with 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol.

Complexes of copper(II) with 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (5-Br-PADAP) formed in aqueous methanol in a wide range of pH (from acidic to alkaline) as well as copper(I)-5-Br-PADAP species formed in methanolic solutions were investigated by spectrophotometry and mass spectrometry. Pseudomolecular and fragment ions created in the atmospheric pressure chemical ionization (APCI) source confirmed the molecular masses of the complexes existing in the solvents and their structures. The structure of the Cu(II) complex with 5-Br-PADAP formed in acidic medium was proposed as CuR(R - H) (where R is the undissociated molecule of the reagent). The binding sites of the two bound reagent molecules were different: in one of them the oxygen atom of the dissociated phenolic group and the nitrogen atom from the azo (-N=N-) group took part in complex formation, whereas in the other only nitrogen atoms from the pyridyl ring and azo group were involved. The complex was stable and could not be reduced to Cu(I) species by use of standard reducing agents (ascorbic acid, hydroxylamine). In alkaline solutions the complex tended to polymerize and precipitated in media containing less than 80% of methanol. The copper(I)-5-Br-PADAP complex was extremely unstable and could be obtained (as a mixture with Cu(II) species) in media free of water or oxygen. For this complex, CuR(2) was proposed as the most probable structure. According to this proposal copper(I) reacted exclusively with nitrogen-containing binding sites and the undissociated phenolic group was not engaged in complex formation. In this system Cu(I)/Cu(II) electron transfer is very rapid, accelerated by a polar environment, e.g. in the presence of water molecules or dissolved oxygen.

Journal Article↗
PubMed2000

Tobacco smoking and vitamin C concentration in gastric juice in healthy subjects and patients with Helicobacter pylori infection.

Low gastric juice total vitamin C concentration in the presence of Helicobacter pylori infection probably plays a role in gastric carcinogenesis. Vitamin C plays a role in the neutralization of various pathogenic factors connected with H. pylori infection, including the destruction of free radicals, which damage tissues and cell DNA, and inhibition of the formation of N-nitroso compounds, which have a strong carcinogenic activity. The aim of the study was to determine whether tobacco smoking had any effect on gastric juice vitamin C concentration in healthy subjects and in patients infected with H. pylori. Eighty-six patients with dyspeptic symptoms undergoing routine endoscopy entered the study after giving informed consent. In all patients plasma and gastric juice total vitamin C levels were measured by a spectrophotometric method. They were entered into four groups: group I (controls) - H. pylori-negative non-smokers (n = 17), group II - H. pylori-negative smokers (n = 16), group III - non-smokers with H. pylori infection (n = 21), and group IV - H. pylori-infected smokers (n = 32). In the control group (I) the mean gastric juice total vitamin C concentration was 17.1 microg/ml (range 5.3-40.0 microg/ml), which was significantly higher (P < 0.05) than in group II (12.6 microg/ml, range 5.1-21.0 microg/ml), group III (5.8 microg/ml range 2.1-13.7 microg/ml) and group IV (3.9 microg/ml, range 1.1-10.6 microg/ml) (P < 0.001). Statistically significant differences also were noted between groups II and III (P < 0.01) and groups II and IV (P < 0.001) and between groups III and IV (P < 0.05). These results demonstrate that the concentration of vitamin C in gastric juice is significantly lower in smokers than in non-smokers. This was observed in healthy subjects as well as H. pylori-infected patients. This phenomenon may be one of the mechanisms whereby smoking contributes to the production of gastric lesions, impairs healing of peptic ulcers and also increases the recurrence rate of peptic ulcers in cases with H. pylori infection.

Adult↗
PubMed2000

[Interactions between food and drugs. 1. Malabsorption].

The paper overviews food-drug interactions. The part 1 reviews the interactions of foods with the many different agents which occur at the level of absorption, considering the mechanisms involved. The food-drug interactions can cause an increase, decrease, or delay in the bioavailability of the drugs. There is an aspect of therapy which may have an important clinical repercussion, which it must not be forgotten.

Biological Availability↗
PubMed2000

[Interactions between food and drugs. 2. Disturbances of drug metabolism and synergistic mode of action].

The paper overviews food-drug interactions. The part 2 reviews the interactions of foods with the drugs which occur at the level of metabolism. Concomitant intake with food increases or decreases the concentrations of many agents in humans. The effect seems to be mediated mainly by suppression of the cytochrome P450 in the liver and in the small intestine wall. This results in a diminished first pass metabolism with higher bioavailability and increased maximal concentrations of substrates of this enzyme. To avoid the interactions many drugs should not be taken with the meal and citrus juices, particularly grapefruit juice.

Biological Availability↗
PubMed1998

Sensitive reversed-phase liquid chromatographic determination of fluoride based on its ternary systems with zirconium(IV) or hafnium(IV) and 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol.

Optimum conditions for the direct reversed-phase LC determination of fluoride based on the ternary M-(F-)-(5-Br-PADAP) complexes [M = ZrIV or HfIV and 5-Br-PADAP = 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol] were evaluated. Chromatographic separation was performed with C18 end-capped column with an eluent consisting of acetonitrile-water (85 + 15 v/v) mixture of pH 4.0 +/- 0.3 (flow rate 1 ml min-1), and the eluate was monitored spectrophotometrically at lambda max = 585 nm. The calibration curves were linear over a wide range of fluoride concentrations: from 1 to 110 and 150 ng ml-1 for the ZrIV-(F-)-(5-Br-PADAP) and HfIV-(F-)-(5-Br-PADAP) systems, respectively (using a 20 microliters loop). Under such conditions the detection limits were 0.8 and 0.7 ng ml-1, respectively, and the quantification limit is 1.0 ng ml-1 for both methods. When a 100 microliters loop was used, the limits of both detection and quantification in the method based on the zirconium system were 0.2 ng ml-1. Using the proposed method, fluoride was determined directly in tap water, saliva and an anti-cancer agent for prostatic cancer (Leuprolid).

Antineoplastic Agents, Hormonal↗
PubMed1998

Effects of high dose vitamin C treatment on Helicobacter pylori infection and total vitamin C concentration in gastric juice.

Low gastric juice total vitamin C concentration in the presence of Helicobacter pylori (H. pylori) infection probably plays a role in gastric carcinogenesis. In vitro vitamin C has been shown to inhibit the growth of H. pylori. The aims of this study were to determine the effect of high dose vitamin C administration on H. pylori infection and on gastric juice total vitamin C concentration in patients with H. pylori related chronic gastritis. Sixty patients with dyspeptic symptoms and proven chronic gastritis and H. pylori infection, who were undergoing routine endoscopy, entered the study after giving informed consent. They were randomly coded into two treatment groups. Group 1 (controls, n = 28) were treated with antacids for 4 weeks and Group 2 (n = 32) received vitamin C 5g daily also for 4 weeks. Nine patients did not complete the study and were excluded. Plasma and gastric juice total vitamin C levels were measured at baseline, at the end of 4 weeks treatment and again 4 weeks after treatment cessation. In the control group H. pylori infection remained unchanged in all 24 patients throughout as did the mean gastric juice total vitamin C concentration. However, in the vitamin C treated group eight of 27 patients (30%) who completed the treatment course the H. pylori infection was eradicated (P = 0.01). In these patients the mean gastric juice total vitamin C concentration rose significantly from 7.2 +/- 1.6 micrograms/ml after 4 weeks treatment (P < M 0.001) and 19.8 micrograms/ml 4 weeks after treatment was discontinued (P < 0.001). In the remaining 19 patients with persistent H. pylori infection, the mean gastric juice total vitamin C concentration rose less than in those with successful H. pylori eradication; 6.3 +/- 1.7 micrograms/ml before treatment, 10.8 +/- 1.5 micrograms/ml after 4 weeks treatment (P < 0.05) and a return to pre-treatment levels (7.1 +/- 2.7 micrograms/ml) 4 weeks after vitamin C intake stopped. There were no side effects of vitamin C treatment. This study has shown that 4 weeks daily high dose vitamin C treatment in H. pylori infected patients with chronic gastritis resulted in apparent H. pylori eradication in 30% of those treated. In those patients there was also a highly significant rise in gastric juice total vitamin C concentration which persisted for at least 4 weeks after the treatment ceased. A significant, though less marked, gastric juice total vitamin C concentration increase was observed during vitamin C treatment even in subjects with persistent H. pylori infection, though this was not maintained after treatment ended. The mechanism whereby vitamin C treatment appeared to result in H. pylori eradication is unclear. Further confirmatory studies are indicated.

Adolescent↗
PubMed1997

[A case of ascites in the course of acute pancreatitis].

The case of acute alcoholic pancreatitis, complicated by internal pancreatic fistula is being reported. The general patients symptom in initial illness period was ascites in peritoneal cavity. Biochemical investigation of ascites has shown elevated activity of amylase and protein which is typical for pancreatic ascites. The late diagnosis of ascites cause (in 3rd week of the illness) and complications decreased efficacy of the treatment used (parenteral nutrition and somatostatin). Thus worse the course of the illness.

Acute Disease↗
PubMed1997

[A case of chronic calcifying pancreatitis in a 19 year old man].

We present an advanced case form of chronic calcifying pancreatitis (ch.c.p.) of 19's age man, whose first clinical symptoms appeared when he was 5 years old. For the rest 14 years his complains were diagnosed as vegetative neurosis. The patient was admitted in to the Department in 4th day of severe abdominal pain. High increase of serum and urine amylase activity, changes in USG and CT examinations (20 mm wide pancreatic duct with numerous calcified stones) were find. Acute pancreatitis in the course of ch.c.p. was diagnosed. After typical treatment abdominal pain disappeared and results of laboratory tests achieved a normal range. During 1.5 year follow up (diet, pancreatic enzymes) substitution abdominal pain did not appear and the body weight increased 8 kg.

Abdominal Pain↗
PubMed1996

[Microproteinuria and circadian rhythm of blood pressure in patients with arterial hypertension].

Microproteinuria is a recognized sign of early nephropathy in the course of arterial hypertension. There is few data concerning the excretion of proteins other than albumin in this group of patients. The aim of the study was to examine circadian rhythm of alfa-l-microglobulin (AlMG), albumin (ALB), immunoglobulin G (IgG) excretion and N-acetyl-beta-glukosaminidase (beta NAG)-activity, then to compare the results with the results of 24 hour ambulatory monitoring of arterial blood pressure in patients with arterial hypertension. The study comprised 28 patients. The control group included 27 healthy volunteers. Albumin concentration was determined by Beckman ICS2 nephelometer, using Beckman and Dako reagents. Blood pressure and ECG were monitored by analysis with ABP-system (AMP-USA). In patients with arterial hypertension significantly higher levels of ALB, AlMG, IgG and increased beta NAG activity were observed in morning urine samples. Despite hypotensive treatment blood pressure values were slightly, though significantly higher than in the control group. Among patients in the study circadian BP rhythm was disturbed. The results obtained suggest that in this group of patients subclinical nephropathy develops involving renal glomeruli and proximal tubules--probably resulting from vascular and humoral disorders, with accompanied hypertension.

Acetylglucosaminidase↗
PubMed1996

Variability of modes of division of the superior lobar bronchi in the right and left lungs.

The studies were carried out on 100 right lungs and 100 left lungs taken from cadavers of humans of both sexes, aged 18 to 80 years. After swilling, the bronchi were injected with 65% duracryl and after 24 hours digested in sulphuric acid. The obtained casts were measured and the modes of division of superior lobar bronchi were determined. The results of observation and measurements were presented in the form of schematic drawings and documented with photographs. On the basis of the modes of branching of superior lobar bronchi three types and eight variants of division for the right lobar bronchus and two types and five variants for the left lobar bronchus were identified. The divergence between the frequency of some modes of division of the studied bronchi, especially according to authors from other countries and very similar results of investigations relating to atypical modes of division of some bronchi, performed in Poland and neighbouring countries, were noted.

Adolescent↗
PubMed1994

[A case of Lesniowski-Crohn disease preceded by Yersinia pseudotuberculosis infection].

In etiopathogenesis of Leśniowski-Crohn disease we consider environmental genetical, immunological and physical factors. Among the bacteria that are of importance in a disease process there are: Jarsinia, tubercle bacillus and para- tubercle bacillus. We are to state that the pro.... of the bacteria per se doesn't influence the initiation of a disease. The initiation is caused by a change of the immune response. The introduced case shows the occurrence of Leśniowski-Crohn disease proceeded by Jarsinia pseudotuberculosis. Thai sequence of occurrence doesn't prove the dependence of an effect upon the cause. What is more-there are many controversial opinions in literature considering Leśniowski-Crohn disease. That is why we would like the introduced case to be a contribution to a discussion on the subject of: Possibility of a distant initiation of a disease by some bacterial infection types.

Adult↗
PubMed1993

[Evaluation of the influence of extrapancreatic factors (undernutrition, cirrhosis) on utilization of amino acids by the pancreas after its stimulation with secretin and cerulein].

We studied the utilization of amino acids by the pancreas after its hormonal stimulation in diseases in which disturbances occur of amino acid and protein metabolism. After 45 minutes of intravenous infusion of secretin (1 CU/kg) and cerulein (75 ng/kg) we found a significantly lower (p 0.05) decrease of amino acid concentration than in healthy individuals, both in chronic pancreatitis and in undernutrition and post-inflammatory cirrhosis with decompensation of liver function. Greatest disturbances in amino acid utilization by the pancreas were found in the case of valine, leucine and isoleucine. The results of studies show certain limitations in the use of this method for the assessment of the exocrine function of the pancreas.

Adult↗
PubMed1992

Pulmonary arteries dimensions and their dependences on the age, the height and body weight.

The studies were carried out on 100 right and 100 left lungs taken from human corpses of both sexes whose age varied from 1 month to 80 years. The material was divided into 3 age groups. The pulmonary arteries were injected 65% duracryl and then digested in sulfuric acid. The specimens obtained were examined to measurement the length and the diameter of the interlobar portion of the pulmonary arteries and their correlation indexes to the age, the length and the body weight. It was certain, that the pulmonary arteries dimensions still grows during man's extrafetal life; the diameter of the right pulmonary artery is usual greater than the left artery. The length of the interlobar portion of the right pulmonary artery varied in both sexes from 5 to 43 mm, and from 6 to 43 mm on the left pulmonary artery. The diameter of the some part of pulmonary arteries varied from 3 to 21 mm on the right side, and from 4.5 to 20 mm on the left side.

Aging↗