Search PubMed⌕ Search

Biomedical subjects

M Jankowski

Publications and source records attributed to M Jankowski.

At least 127 records · Page 7Linked to original sources
PubMedDate not supplied

IgG-subclass-specific antibody reactivity to respiratory syncytial virus polypeptides investigated by western blot.

IgG1 and IgG3 subclass-specific antibody reactivity (ABR) in serum samples obtained from infants and children in relation to acute lower respiratory disease caused by respiratory syncytial virus (RSV) infection was investigated by Western blot. IgG1 ABR was directed against the nucleocapsid polypeptides VPN and VPP as well as against the glycoproteins GP48 (F1) and GP90. IgG3 ABR was directed only against VPN and VPP. In infants, a low IgG1 reactivity against glycoproteins was observed. When serum samples obtained in the early convalescent phase were tested, ABR against GP48 and GP90 as well as against VPP differed with respect to RSV subtypes A and B. IgG1 ABR increased in the late convalescent phase, while IgG3 ABR decreased during this phase when serum samples from primary infections were tested.

Antibodies, Viral↗
PubMedDate not supplied

Sensitive enzyme immunoassay for the rapid diagnosis of influenza A virus infections in clinical specimens.

Samples of nasopharyngeal secretion (NPS) from 100 infants and small children admitted for acute respiratory disease during the period from January to March 1989 were examined for the presence of influenza A virus. All samples were tested by enzyme immunoassay (EIA), fluorescent antibody (FA) technique and by isolation in cell culture 3-6 h after they were obtained from the patients. Of 24 influenza strains found by isolation, 21 were detected by EIA and 19 were FA+. In comparison with virus isolation, EIA gave the following values: sensitivity 88%, specificity 100%, positive prognostic value (PPV) 100%, and negative prognostic value (NPV) 96%. A rabbit anti-influenza-A serum (A-13) was used as catching antibody and a monoclonal anti-influenza-A pool against NP protein was used as detector antibody in EIA. A-13 gave bands corresponding to influenza A core proteins (NP and M1) in Western blot (WB) studies when different H3N2 strains were employed as antigens. A-13 gave only a band corresponding to the NP protein when H1N1 strains were examined by WB. The detection level by EIA for both H3N2 and H1N1 strains precipitated by polyethylene glycol from tissue culture maintenance medium was 1-2 ng.

Antigens, Viral↗
PubMedDate not supplied

Studies on potential involvement of protein kinase C in glomerular insensitivity to atrial natriuretic factor on low sodium intake.

BACKGROUND: Atrial natriuretic factor (ANF)-induced increase in glomerular filtration rate (GFR) is inhibited on low sodium intake. It has been shown that activation of renin-angiotensin system on low sodium intake antagonizes the biological effect of ANF by interfering in the intracellular metabolism of cGMP. We have previously indicated that the renin-angiotensin system increases activity of Ca2+/calmodulin dependent-cyclic GMP phosphodiesterase (cGMP-PDE) in glomeruli and thereby inhibits the ANF-induced increase in GFR in low sodium-treated rats. The aim of the present study was to investigate whether low sodium intake might change glomerular cGMP metabolism by the alternative branch of the signal transduction pathway, namely protein kinase-C (PKC) activation. MATERIAL AND METHODS: cGMP formation and PKC activity were examined in isolated glomeruli from the rats maintained for five days on a normal or a low sodium diet. Renal hemodynamic parameters in clearance experiments during infusion of ANF (0.5 Kg/min/kg body weight) in both groups of rats were also evaluated. RESULTS: Low sodium intake inhibited ANF-dependent increase in GFR and nephrogenous cGMP excretion, whereas urinary sodium excretion did not differ appreciably in rats on either diet. The basal and ANF-stimulated cGMP formation in isolated glomeruli was significantly inhibited in low sodium-treated rats as compared to normal sodium-treated rats. The inhibitory effect of low sodium intake on basal and ANF-stimulated glomerular cGMP formation was completely prevented by a selective cGMP-PDE inhibitor, zaprinast, but not affected by PKC activator, PMA, or PKC inhibitor, H-7. The activity of PKC in glomeruli neither in membrane fraction nor in cytosol fraction did not differ significantly between normal and low sodium-treated rats. CONCLUSIONS: These results demonstrate that the blunted glomerular response to ANF in rats on low sodium intake is due to decrease ability of cGMP formation in glomeruli by increasing activity of cGMP-PDE without altering activity of PKC.

3',5'-Cyclic-GMP Phosphodiesterases↗
PubMedDate not supplied

The role of P2Y-receptors in the regulation of glomerular volume.

BACKGROUND: Extracellular ATP signaling affects the cells of renal glomeruli via activation of P2-purinoceptors, denoted as P2X and P2Y. Through either of these purinoceptors, ATP is able to stimulate an increase in intracellular [Ca2+]. P2Y-receptors are expressed on mesangial and endothelial cells, thus may participate in contraction and relaxation of glomeruli, respectively. Moreover, P2Y-receptors possess activity of ecto-ATPase which may lead to dephosphorylation of ATP and generation of adenosine. The aim of the present study was to investigate the involvement of P2Y-receptors in responses of renal glomeruli to extracellular ATP. MATERIAL AND METHODS: Renal glomeruli were isolated from rats by sieving technique. [3H]-inulin was used to measure the intracapillary volume of isolated glomeruli. Changes of intracapillary volume reflect contraction and relaxation of the glomeruli. ATP and adenosine concentration in the incubation mixture were measured using luminometric methods. RESULTS: Extracellular ATP (1 microM) induced relaxation of Ang II-precontracted glomeruli in time-dependent manner. The glomeruli relaxed completely at 2nd minute of incubation. The relaxation was considerably diminished at 5th minute of incubation as compared to 2nd minute. Relaxing effect was completely prevented by an antagonist of P2Y-receptors i.e. reactive blue 2. The decrease in ATP concentration with time was accompanied by a rise in adenosine concentration which led to contraction of glomeruli. Non-metabolised analogue of ATP, an agonist of P2Y-receptors i.e. 2-methylthio-ATP (1 microM) induced complete relaxation at 2nd minute of incubation but there was no effect at 5th minute of incubation. CONCLUSIONS: The extracellular ATP through activation of P2Y-receptors may regulate the volume of renal glomeruli, which in turn influences on the glomerular filtration rate, through at least two mechanisms: one is ATP-dependent glomerular relaxation in the initiate phase and the other is glomerular contraction caused by either ATP itself or adenosine formed from ATP hydrolysis in maintenance phase.

Adenosine Triphosphate↗
PubMedDate not supplied

Antithrombotic actions of statins.

Aspirin depresses thrombin generation, probably through a mechanism independent of the cyclooxygenase inhibition, but rather related to acetylation of the platelet membrane macromolecules. This action of aspirin is blunted in hypercholesterolemia. In men with marked hypercholesterolemia, lowering serum cholesterol by a three-month simvastatin treatment is accompanied by a reduction of thrombin generation both at basal conditions in venous blood and after activation of hemostasis by microvascular injury. Similar results are obtained in patients with coronary heart disease and borderline - high cholesterol levels. We assessed tissue-factor initiated coagulation in blood samples collected every 30-seconds from bleeding time wounds in patients with advanced coronary artery disease and total cholesterol levels of 224 mg/dL. Three-month simvastatin treatment depressed blood clotting, leading to reduced rates of prothrombin activation, FVa generation, fibrinogen cleavage, FXIII activation, and an increased rate of FVa inactivation. Such a concerted influence of statins on the clotting cascade seems to be independent of their lipid-lowering action and may be the result of depressed isoprenoid production.

Anticholesteremic Agents↗
PubMedDate not supplied

[Clinical aspects of herpes simplex meningoencephalitis].

On the basis of the observed 10 cases the authors discuss the diagnostic, clinical and therapeutic problems of herpes encephalomeningitis. The diagnosis in early phase of the disease but already after appearance of psychic and neurological symptoms and signs is based on clinical criteria and also on the result of examination of the cerebrospinal fluid and rapid serological method ELISA. In view of the already available possibilities of virostatic treatment early diagnosis is of utmost importance, for beginning treatment (preferably with Vidarabine) before the development of extensive necrotic brain lesions.

Adult↗