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Biomedical subjects

M Jackson

Publications and source records attributed to M Jackson.

At least 343 records · Page 19Linked to original sources
PubMed1984

Organization and expression of non-Alu family interspersed repetitive DNA sequences in the mouse genome.

The mouse genome is complex with regard to DNA sequence organization and transcriptional activity. To more fully understand the role of interspersed repetitive DNA sequences we have isolated and characterized five different mouse non-Alu DNA sequence families. We have found that: (1) the distribution of repetitive sequences is non-random in the genome; (2) two of the five families (Bam5 and R) were previously described by Fanning (1982) and Gebhard et al. (1982), respectively. We found that these two families are linked to each other and are found adjacent to seven of seven studied structural genes but in randomly selected DNA fragments showed much less significant linkage. (3) The position of the Bam5 and R family repeat units relative to beta-globin and relative to a housekeeping gene has been evolutionarily conserved in mice and humans. (4) Three previously undescribed families representing from 200 to 40,000 copies per genome have been characterized and shown to have equivalent human sequences. (5) All five families studied are represented in RNA polymerase II transcripts. Little RNA polymerase III transcription homologous to these three families could be detected. The structural and functional features of these five families defined in this paper provide a basis for studies on the functional role of interspersed repetitive DNA in the mouse.

Animals↗
PubMed1984

A halo-caliper guidance system for bi-maxillary (dual-arch) orthognathic surgery.

This paper reports a simple method for localisation of both maxilla and mandible into planned new positions when both jaws are to be freed during bi-maxillary orthognathic surgery. A three stage system using a halo frame and a detachable caliper of one eighth inch rod and two universal joints is described for use with either silver cap splint or orthodontic arch wire fixation.

Adolescent↗
PubMed1984

Spenolimycin, a new spectinomycin-type antibiotic. I. Discovery, taxonomy and fermentation.

Spenolimycin, a new spectinomycin-type antibiotic, was discovered in the fermentation broth of a new actinomycete named Streptomyces gilvospiralis sp. nov. strain AB634D-177. Although a small amount of spectinomycin is coproduced by strain AB634D-177, the culture is different from other known spectinomycin-producing actinomycetes. Spenolimycin was obtained by conventional submerged culture in 14-liter fermentors with a peak antibiotic titer of 140 micrograms/ml.

Fermentation↗
PubMed1984

Promotion and tenure in family practice in US medical schools.

Academic development is recognized as a necessary requirement in family medicine and can be measured in part by assessing the experience with the promotions and tenure process in university departments. In this study a questionnaire was distributed to the chairmen of departments of family practice with direct medical school affiliations. The chairmen were asked to describe the current rank profile of the faculty in their departments and their perception of the importance of various criteria on promotion and tenure decisions at their school. The departments averaged 1.8 full professors (72 percent tenured), 3.0 associate professors (40 percent tenured), 6.1 assistant professors (5 percent tenured), and 1.5 instructors (none tenured). Research quality and quantity were considered the most important positive influences on promotions and tenure decisions. Teaching skills were slightly less important, and patient care and administrative skills were not important influences. This project demonstrates that (1) family practice faculties are relatively underdeveloped with respect to senior, tenured positions, (2) research is highly important to achieve promotion and tenure, but little time is available to the faculty to do that research, and (3) the needs of the departments in the areas of patient care and administration may conflict with the requirements for promotion and tenure.

Faculty, Medical↗
PubMed1983

Enhanced polypeptide synthesis programmed by linear DNA fragments in cell-free extracts lacking exonuclease V.

Preparation of an in vitro coupled transcription-translation system from E. coli strains lacking exonuclease V has greatly improved the system for use with added linear DNA fragments. In fact, in extracts of these mutants linear fragments are stable for several hours. However the cell extracts show a high level of endogenous background. To avoid this complication extracts were prepared at 30 degrees C from a mutant carrying a temperature-sensitive exonuclease V. Polypeptides coded by a specific DNA region, e.g., delineated by restriction endonuclease sites, can now be easily identified.

DNA, Bacterial↗
PubMed1983

A product report: Osto-Zyme as an odor eliminator.

A product, Osto-Zyme, was clinically tested at our Institute for the elimination of odor. Thirteen clients were chosen by enterostomal therapists to be tested. The product was sprayed directly into pouch, peristomal skin, and into night drainage units. This product proved very effective in the elimination of odor. The manufacturer of Osto-Zyme is the Richard C. Shelton Co., Wright Brothers Branch, Dayton, Ohio.

Aged↗
PubMed1983

Binding of 1-nitro[14C]pyrene to DNA and protein in cultured lung macrophages and respiratory tissues.

Binding of 1-nitro[14C]pyrene (1-NP) or its metabolites to cellular DNA and protein in cultures of rabbit alveolar macrophages and lung and tracheal tissues was examined. DNA binding was highest in tracheal tissue (136.9 +/- 18.3 pmol 1-NP/mg DNA). DNA binding in macrophages and lung tissue was one-fifth of the level observed in tracheal tissue. Also, 1-NP was bound to cellular protein in tracheal and lung tissues, and at a lower level in macrophages. Co-cultivation of the macrophages with lung and tracheal tissues decreased the DNA binding in tracheal tissue and increased the protein binding in macrophages. This study shows that lung cells and tissue are capable of binding 1-NP or its metabolites to DNA and protein.

Animals↗
PubMed1983

Social support networks of the elderly: racial differences and health care implications.

Interest in social support networks has increased in recent years. A configuration of family, friendships, neighbors, neighborhood groups, comprise the social support network of individuals and families. Other entities referred to in social network of individuals and families. Other entities referred to in social support literature include self-help and mutual help organizations. Interest in descriptive studies of social support networks includes both structural and interactional factors. In analyses of structural factors in social support networks, the focus is most frequently on size (number of persons), density (proportion of linkages) and centrality (extent of accessibility).

Black or African American↗
PubMed1983

Child's play.

Explore the source record for details and available documents.

Child Day Care Centers↗
PubMed1982

Insertion of a MalE beta-galactosidase fusion protein into the envelope of Escherichia coli disrupts biogenesis of outer membrane proteins and processing of inner membrane proteins.

The synthesis of a membrane-bound MalE beta-galactosidase hybrid protein, when induced by growth of Escherichia coli on maltose, leads to inhibition of cell division and eventually a reduced rate of mass increase. In addition, the relative rate of synthesis of outer membrane proteins, but not that of inner membrane proteins, was reduced by about 50%. Kinetic experiments demonstrated that this reduction coincided with the period of maximum synthesis of the hybrid protein (and another maltose-inducible protein, LamB). The accumulation of this abnormal protein in the envelope therefore appeared specifically to inhibit the synthesis, the assembly of outer membrane proteins, or both, indicating that the hybrid protein blocks some export site or causes the sequestration of some limiting factor(s) involved in the export process. Since the MalE protein is normally located in the periplasm, the results also suggest that the synthesis of periplasmic and outer membrane proteins may involve some steps in common. The reduced rate of synthesis of outer membrane proteins was also accompanied by the accumulation in the envelope of at least one outer membrane protein and at least two inner membrane proteins as higher-molecular-weight forms, indicating that processing (removal of the N-terminal signal sequence) was also disrupted by the presence of the hybrid protein. These results may indicate that the assembly of these membrane proteins is blocked at a relatively late step rather than at the level of primary recognition of some site by the signal sequence. In addition, the results suggest that some step common to the biogenesis of quite different kinds of envelope protein is blocked by the presence of the hybrid protein.

Bacterial Outer Membrane Proteins↗
PubMed1981

Response of unmarried adolescents to contraceptive advice and service in Nigeria.

The responses of a group of all unmarried, sexually active teenagers in a developing country to offers of contraceptive service were assessed in a prospective, 30-month study at a teaching hospital family planning center. Teenagers of this group constituted 7.2% of the clinic population. The default rate was very high (43.0%) and was most noticeable among users of oral contraceptives. The intrauterine device seemed more acceptable, as were injections of norethisterone enanthate. Possible reasons for this pattern of response are given, and the authors suggest giving new consideration to making the intrauterine device more suitable for and acceptable to teenagers of developing countries. The place of norethisterone enanthate in teenage contraception is also discussed.

Adolescent↗