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Biomedical subjects

M J Wheeler

Publications and source records attributed to M J Wheeler.

61 records · Page 4Linked to original sources
PubMed1978

Oral testosterone, a reappraisal.

A microparticulate form of free testosterone was given orally to 6 hypogonadal men. Plasma levels were followed after a 200-mg dose, and a double-peak effect was observed. This suggests that particles of different sizes were absorbed at different rates. The clinical and biochemical effects were observed over a 2-month period, on a dose of 200 mg twice daily, taken in place of the usual androgen replacement. The results indicate that absorption is not sufficiently reliable for routine use. The large doses required to achieve therapeutic levels, make oral administration of free testosterone impractical.

Administration, Oral↗
PubMed1978

Quantitative estimation of clinically important monosaccharides in plasma by rapid thin layer chromatography.

A method is described for the quantitative estimation of clinically important monosaccharides in plasma or whole blood by direct densitometry of chromatographically-separated zones on silica gel layers. Simple modifications of technique originally introduced to improve the reproducibility of paper chromatography have now been adapted for thin layers. The present method is based on peak height measurement with an internal marker correction. Galactose, fructose, D-xylose, and 3-O-methyl glucose can be estimated in addition to glucose, either singly or in combination, within three or four hours, using an initial sample volume of 0.5 ml. With reasonable experience and skill a coefficient of variation of 3 to 6%, depending on sugar concentration, can be achieved without replication, and the limit of sensitivity is about 0.05 mmol/l. When the performance was compared with an automated glucose oxidase/peroxidase system for glucose and the recovery for the other monosaccharides was calculated, the results were satisfactory.

Blood Glucose↗
PubMed1975

Comparison of radioimmunoassay methods for human luteinising hormone.

1. Four radioimmunoassay methods for the determination of luteinising hormone in serum or urine are described. 2. Evaluation of these methods (one employing laboratory prepared reagents, three utilizing commercial kits) was made to assess their routine laboratory value. Comparable precision and accuracy were found. Great economy is achieved by pruchasing LH and anti-LH preparations and by labelling one's own hormone. 3. Suggestions for future improvements in kit LH assay control and unitage are made.

Animals↗