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Biomedical subjects

M J Wagner

Publications and source records attributed to M J Wagner.

At least 73 records · Page 4Linked to original sources
PubMed1983

Transcription of herpes simplex virus genes in vivo: overlap of a late promoter with the 3' end of the early thymidine kinase gene.

We identified in herpes simplex virus type 1-infected cells six cytoplasmic transcripts which were complementary to BamHI restriction endonuclease fragment Q. Two transcripts appeared in major amounts compared with the other four. One major transcript of about 1.4 kilobases was the mRNA for the viral thymidine kinase, was synthesized at intermediate times, and was classified as a beta transcript. The other major transcript was synthesized at late times and was classified as a gamma transcript. This late transcript was about 3 kilonucleotides long and was transcribed in the same direction as the gene for thymidine kinase. The 5' end of this late RNA was located by RNA sequence analysis and was 23 nucleotides downstream from the polyadenylation site for the thymidine kinase mRNA. This finding led to the conclusion that the control region for the 3-kilobase gamma transcript is contained within the 3' untranslated region of the thymidine kinase transcript.

Base Sequence↗
PubMed1981

Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.

We have determined the complete nucleotide sequence of the thymidine kinase (ATP:thymidine 5' phosphotransferase, EC 2.7.1.21) gene of herpes simplex virus type 1 strain CL101 from a plasmid clone of viral DNA derived by Enquist et al. [Enquist, L. W., Vande Woude, G. F., Wagner, M., Smiley, J. R. & Summers, W. C. (1979) Gene 7, 335-342]. A cDNA copy of the 5' end of thymidine kinase mRNA was also analyzed to locate the transcribed sequences. The transcribed portion of the gene is approximately 1300 nucleotides in length and appears to contain no intervening sequences. There is an untranslated region of 107 nucleotides at the 5' end of the mRNA followed by an open reading frame of 1128 nucleotides. The gene is thus capable of coding for a protein of 376 amino acids. Sequences similar to those thought to be involved in control of transcription and translation of a variety of eukaryotic and viral genes such as a "Hogness box" and A-A-T-A-A-A polyadenylylation signals are also present in the herpesvirus thymidine kinase gene.

Amino Acid Sequence↗
PubMed1978

Structure of the joint region and the termini of the DNA of herpes simplex virus type 1.

Analysis of restriction endonuclease cleavage sites within the inverted, repeated sequences in the joint region of the DNA of herpes simplex virus type 1 strain KOS revealed the presence of two types of sequence heterogeneity. The first was an insertion of 280 base pairs or multiples of 280 base pairs which was found in approximately half of all DNA molecules from every plaque-purified stock of virus. These insertions seemed to be tandem duplications of sequences which were present at the joint and correspond closely to the inverted terminal redunancy. The second type of heterogeneity was due to variable insertions and deletions which were present in some, but not all, plaque-purified virus stocks. Comparison of restriction fragments from the joint region with fragments from the termini indicated that in the simplest observed molecules of herpes simplex virus type 1 DNA, only one copy of the inverted terminal redundancy was present at the joint. A map of restriction endonuclease cleavage sites in the joint region is presented.

Base Sequence↗
PubMedDate not supplied

Objectives to direct the training of emergency medicine residents on off-service rotations: surgery, Part 3.

This is the 36th and final article in a series of objectives to direct emergency medicine resident experiences on off-service rotations. An understanding of the principles of surgical diagnosis and treatment is an essential component of the practice of emergency medicine. Emergency medicine residents rotating on surgical services require specific objectives to maximize their learning potential, emphasize early patient assessment, identify the possible need for surgery, and gain a basic understanding of definitive management. This article approaches surgical problems from the presenting complaint. It concludes with procedures not covered in the goals and objectives for traumatology.

Curriculum↗
PubMedDate not supplied

Clinical anatomy: a review course for emergency medicine residents.

An understanding of anatomy is essential to the practice of Emergency Medicine. A significant amount of anatomy knowledge is lost in the transition from student to clinician. This article describes the content and structure of a clinically oriented anatomy review course for senior emergency medicine residents.

Anatomy↗