A PCR assay for the diagnosis of infection produced by Candida sp. was developed. The primers, designated 520 and 522, were selected from highly-conserved areas of the small subunit (ssu) 18S rRNA gene of Candida spp. To check the value of the results a Candida albicans oligonucleotide probe, digoxigenin-labeled, and a general Candida probe were used in hybridization experiments with the amplified products. We were able to detect a Candida- specific fragment of 1800 bp from different clinical samples. The procedure described could provide an interesting complement to present diagnostic methods of detecting Candida sp in clinical samples.

Journal Article↗

PubMed1997

[Pneumonia caused by Rhodococcus equi in patients with AIDS: difficulty in defining the most appropriate initial treatment].

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AIDS-Related Opportunistic Infections↗

PubMed1996

Maxillary sinusitis caused by Nocardia nova.

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Adult↗

PubMed1996

Serratia rubidaea as an invasive pathogen.

Serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who underwent invasive procedures. The infection was cleared after adequate treatment with antibiotics.

Anti-Bacterial Agents↗

PubMed1996