Search PubMed⌕ Search

Biomedical subjects

M J Stuart

Publications and source records attributed to M J Stuart.

At least 37 records · Page 2Linked to original sources

Flexion instability after primary posterior cruciate retaining total knee arthroplasty.

Between 1990 and 1995, 25 painful primary posterior cruciate ligament retaining total knee arthroplasties were revised for flexion instability. These patients shared typical clinical presentations that included a sense of instability without frank giving way, recurrent knee joint effusion, soft tissue tenderness involving the pes anserine tendons and the retinacular tissue, posterior instability of 2+ or 3+ with a posterior drawer or a posterior sag sign at 90 degrees flexion, and above average motion of their total knee arthroplasty. The primary total knee arthroplasty was performed for osteoarthritis in 23 patients and rheumatoid arthritis in two patients. There were 13 male and 12 female patients and their mean age was 65 years (range, 35-77 years). Before the revision operation, Knee Society knee scores averaged 45 points (range, 17-68 points) and function scores averaged 42 points (range, 0-60 points). Twenty-two of the knee replacements were revised to posterior stabilized implants and three underwent tibial polyethylene liner exchange only. Nineteen of the 22 knee replacements revised to a posterior stabilized implant were improved markedly after the revision surgery. Only one of three knee replacements that underwent tibial polyethylene exchange was improved. After the revision for flexion instability, Knee Society knee scores averaged 90 points (range, 82-99 points) and function scores averaged 75 points (range, 45-100 points) for the 20 knees with a successful outcome. This study suggests that flexion instability can be a cause of persistent pain and functional impairment after posterior cruciate ligament retaining total knee arthroplasty. A revision operation that focuses on balancing the flexion and extension spaces, in conjunction with a posterior stabilized knee implant, seems to be a reliable treatment for symptomatic flexion instability after posterior cruciate retaining total knee arthroplasty.

Adult↗

Out of hospital cardiac arrest and associated injury.

Three patients are described who sustained injuries around the time of a collapse that led to out of hospital cardiac arrest. In this group of patients the importance of taking a complete medical history and recording the circumstances of the syncopal episode cannot be overemphasised. If cardiac output is successfully restored the possibility of occult traumatic injury must be considered in high risk patients.

Aftercare↗

Tardy sciatic nerve palsy following apophyseal avulsion fracture of the ischial tuberosity. Case report.

This 4 1-year-old man presented with a 2-year history of symptoms and signs of sciatic nerve compression. Imaging studies revealed a large ossified fragment within the biceps muscle of the thigh abutting the sciatic nerve at the level of the lesser trochanter. The bony fragment resulted from an unrecognized apophyseal avulsion fracture of the ischial tuberosity, which the patient had sustained while sprinting 27 years earlier. External neurolysis of the sciatic nerve and excision of the mass led to a successful outcome.

Adult↗

Psychologic, situational, and physiologic variables and on-ice performance of youth hockey goalkeepers.

OBJECTIVE: To determine the relationship between psychologic, situational, and physiologic variables and on-ice performance of youth hockey goalkeepers. DESIGN: This study was structured to identify relationships and predictors of goalie performance. MATERIAL AND METHODS: Because athletes playing solo positions in team sports have not been analyzed in depth in terms of precompetition anxiety and because goalkeeper performance is critical to game outcome, we undertook a study of 43 goalies at a hockey camp. These goalies completed psychometric inventories to assess trait and state anxiety, confidence, life stress, and social support. Holter monitors measured heart rate while the goalies rotated through on-ice stations. Goalies were videotaped at the puck-shooting machine station, and performance (percent saves) was calculated. RESULTS: Trait (somatic) anxiety and positive mood state (ability to share) had different but significant relationships with on-ice performance. Heart rates ranged from 88 to 208 beats/min at the on-ice stations. Mean heart rate for older goalies (14 to 18 years of age) was 164 beats/min at the puck-shooting machine and 176 beats/min at other stations such as the slap-shot station. CONCLUSION: Older goalies performed well at a high level of arousal. Better performing goalies were more experienced, had faster heart rates "in the net," and had lower scores on all measures of anxiety.

Adolescent↗

Valgus-varus motion of the knee in normal level walking and stair climbing.

OBJECTIVE: The knee valgus-varus moment and the knee angles were compared between normal level walking and stair climbing. DESIGN: Ten healthy subjects were tested for ascent, descent, and level walking. BACKGROUND: An understanding of the normal valgus-varus motion of the knee during stair climbing is needed to apply biomechanical analysis of stair climbing as a evaluation tool for knee osteoarthritis patients. METHODS: A motion analysis system, three force plates, and a flight of stairs were used to collect kinematic and kinetic data. The knee angles and moments were calculated from the collected kinematic and kinetic data. RESULTS: The knee varus angle for the maximum knee valgus moments in stair climbing was significantly greater than that in level walking. The knee valgus moment was significantly correlated to ground reaction forces and knee valgus-varus angle during stair climbing and level walking. CONCLUSIONS: There is a coupling between the knee valgus-varus motion and flexion-extension motion. Ground reaction forces are the major contributors to the within-subject variation in the knee valgus-varus moment during stair climbing and level walking. The knee valgus-varus angle is a major contributor to the between-subject variation in the knee valgus moment during stair climbing and level walking.

Journal Article↗

Periprosthetic fractures of the tibia associated with total knee arthroplasty.

One hundred two periprosthetic tibial fractures associated with total knee arthroplasty were identified in 29 men and 73 women. Eighty-three fractures occurred postoperatively, and 19 occurred intraoperatively. Fractures were classified into four types based on location and proximity to the prosthesis. There were 61 Type I fractures, occurring at the tibial plateau; 22 Type II fractures, occurring adjacent to the prosthetic stem; 17 Type III fractures, occurring distal to the prosthetic stem; and two Type IV fractures, involving the tibial tubercle. Fracture types were additionally classified by whether the prosthesis appeared to be radiographically well fixed (A) or loose (B) at the time of fracture, or whether the fracture occurred intraoperatively (C). The majority of postoperative Types I and II fractures were Types IB and IIB, and these were treated most successfully with revision surgery. Types IIA, IIIA, and IVA fractures were managed successfully by the usual principles of tibial fracture treatment. Type IC fractures usually were managed by intraoperative fixation, Type IIC by bone grafting or external immobilization and weightbearing restrictions, and Type IIIC by conventional fracture management. This classification system provides a guide for determining the appropriate treatment for tibial fractures associated with total knee arthroplasty.

Adult↗

Vascular cell adhesion molecule-1 is involved in mediating hypoxia-induced sickle red blood cell adherence to endothelium: potential role in sickle cell disease.

We investigated the effects of hypoxia on red blood cell (RBC)-endothelial cell (EC) adherence and the potential mechanism(s) involved in mediating this effect. We report that hypoxia significantly increased sickle RBC adherence to aortic EC when compared with the normoxia controls. However, hypoxia had no effect on the adherence of normal RBCs. In additional studies, we found that the least dense sickle RBCs containing CD36+ and VLA-4+ reticulocytes were involved in hypoxia-induced adherence. We next evaluated the effects of hypoxia on the expression of EC surface receptors involved in RBC adherence to macrovascular ECs, including vascular cell adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1), and the vitronectin receptor (VnR). Hypoxia upregulated the expression of both VCAM-1 and ICAM-1, whereas no effect on VnR was noted. Potential involvement of VCAM-1 and ICAM-1 in mediating hypoxia-induced sickle RBC-EC adhesion was next investigated using monoclonal antibodies against these receptors. Whereas anti-VCAM-1 had no effect on basal adherence, it inhibited hypoxia-induced sickle RBC adherence in a concentration-dependent manner, with 50% to 75% inhibition noted at 10 to 60 micrograms/mL antibody (n = 6, P < .05 to P < .01). Anti-ICAM-1 (10 to 60 micrograms/mL, n = 8) had no effect on either basal or hypoxia-induced adherence. As noted in the bovine aortic ECs, hypoxia stimulated the adherence of sickle RBCs to human retinal capillary ECs, and this response appeared to be mediated via mechanisms similar to those observed with macro-endothelium, ie, via the adhesive receptor combination VCAM-1-VLA-4. Our studies show that hypoxia enhances sickle RBC adhesion to both macrovascular and human microvascular ECs via the adhesive receptor VCAM-1. Our findings are of interest because hypoxia is an integral part of the pathophysiology of the vaso-occlusive phenomenon in sickle cell anemia.

Anemia, Sickle Cell↗

Sickle red blood cells stimulate endothelial cell production of eicosanoids and diacylglycerol.

The effects of sickle red cell-endothelial cell interaction on endothelial cell arachidonic: acid (AA) mobilization, eicosanoid release, and diacylglycerol (DAG) production were evaluated by using bovine aortic endothelial cells. We have shown that coincubation of washed red blood cells (RBCs) from patients with sickle cell disease with endothelial cells stimulate AA release (90% increase as compared with buffer controls, n = 8, p < 0.002). Released AA was mobilized from membrane phosphatidylcholine and phosphatidylserine and was converted to eicosanoids via the cyclooxygenase and lipoxygenase pathways in increased amounts in the presence of sickle erythrocytes. The production of prostacyclin and 15-hydroxyeicosatetraenoic acid (15-HETE) were increased by 78% (p < 0.01) and 103% (p < 0.025), respectively, as shown by both chromatographic and immunoassay procedures. Sickle erythrocytes also stimulated the hydrolysis of endothelial cell phosphoinositides, including phosphatidylinositol-mono-phosphate (p < 0.03) and phosphatidylinositol-bis-phosphate (p < 0.006). This response was accompanied by a significant increase in the production of DAG (50% increase as compared with buffer control, n = 8, p < 0.025). In contrast, coincubation of washed erythrocytes from normal healthy donors with endothelial cells had no significant effect on endothelial cell phospholipid turnover. When the sickle RBC-induced biochemical changes in endothelial cells were contrasted with those observed with normal RBCs, the ability of sickle RBCs to induce AA mobilization and the production of mono-HETEs and DAG was markedly increased (p = 0.05 to p < 0.025). Because 15-HETE is a pro-adhesinogenic eicosanoid and DAG is an endogamous activator of protein kinase C, an enzyme involved in modulating cell surface adhesive properties, both 15-HETE and DAG could potentially play a role in the vascular pathophysiology of sickle cell disease.

Anemia, Sickle Cell↗

Impaired mobilization of intracellular calcium in neonatal platelets.

Abnormalities in platelet functions including aggregation and the release reaction have long been recognized to be present in neonatal platelets. Because calcium is an important mediator of many platelet functions, we have investigated the mobilization of calcium in neonatal platelets. All umbilical cord blood samples were obtained from healthy, full term gestations. Changes in cytoplasmic calcium levels were monitored using Fura-2 as a fluorescent probe. Fura-2-loaded washed platelets were stimulated with the agonists collagen (2 micrograms/mL) or thrombin (1.0 U/mL). When compared with adult controls, intracellular calcium release in the platelets of the neonate was significantly impaired in response to these agonists. Mean levels for calcium release in adults versus neonates in response to collagen were 168 +/- 120 nM (+/-SD, n = 10), and 61 +/- 69 nM (n = 7, p < 0.05). A decrease in response to thrombin was also observed [1296 +/- 503 nM (n = 8) in adults versus 603 +/- 482 nM (n = 7) in neonates, p < 0.025]. Results similar to those observed with unpaired neonatal and adult platelets were also obtained when neonatal platelets (n = 5) were compared with their paired maternal controls. In further studies, we have documented that the calcium content of the dense tubular system was normal in the neonatal platelet, indicating that the observed impairment in calcium mobilization in the neonate was not due to a decrease in calcium stores. The previously documented abnormalities in neonatal platelet function appear to be due to the impaired mobilization of this important intracellular mediator.

Adult↗

Ultrasound assessment and conservative management of inversion injuries of the ankle in children: plaster of Paris versus Tubigrip.

We studied 45 children who presented with an inversion injury of the ankle. The clinical signs suggested injury to the distal growth plate of the fibula, but the plain radiographs appeared normal. Ultrasound examination of the joint in 40 patients showed a subperiosteal haematoma consistent with a growth-plate injury in 23 (57.5%). Children who had been treated with a tubular bandage and crutches by random selection had a mean time to return of normal activity of 14.22 days compared with 21.60 days for those treated with a plaster-of-Paris cast (t=3.60, p=0.0032; d=7.38, 95% CI 3.0 to 11.8). We conclude that children with inversion ankle injuries who have clinical signs of injury to the distal fibular growth plate but a normal radiological appearance, should be treated with a tubular bandage and crutches.

Activities of Daily Living↗

Fetal bleeding in neonatal alloimmune thrombocytopenia mediated by anti-PlAl is not associated with inhibition of fibrinogen binding to platelet GPIIb/IIIa.

Antibody directed against the platelet-specific alloantigen, PlAl, is the most frequently reported cause of two syndromes, post-transfusion purpura (PTP), and neonatal alloimmune thrombocytopenia (NAIT). Numerous reports have indicated that anti-PlAl also has the ability to block certain responses of platelets to stimulation, including fibrinogen binding, platelet aggregation, and serotonin release. Because the PlAl epitope is located on platelet membrane glycoprotein (GP) IIb/IIIa that also contains the fibrinogen receptor, these effects may be mediated by antibody binding at or near the fibrinogen receptor site. This study examines the capacity of anti-PlAl from patients with PTP and from mothers of infants affected by the NAIT to block the binding of radio-labeled fibrinogen to washed human platelets stimulated by ADP and epinephrine. In six of the seven PTP patients, there was inhibition of fibrinogen binding, ranging from 28% to 84% inhibition. In contrast, all anti-PlAl sera from nine mothers of infants with NAIT, including four with intracranial hemorrhage, failed to inhibit fibrinogen binding. Despite the generally higher anti-PlAl titers of the PTP sera, the ability to inhibit fibrinogen binding did not appear attributable to antibody titers. These results suggest that interference with fibrinogen binding to platelets by maternal anti-PlAl does not underlie the increased risk of bleeding in NAIT, whereas inhibitory activity directed against fibrinogen binding appears to be a characteristic feature of the sera from PTP patients.

Antigens, Human Platelet↗

Sickle cell vaso-occlusive crisis is associated with abnormalities in the ratio of vasoconstrictor to vasodilator prostanoids.

Plasma levels of 6-keto-prostaglandin F1 alpha (6kPGF1 alpha) and thromboxane (Tx) B2 have been assessed in sickle cell disease (SCD) with discrepant results. Inasmuch as direct measurement of plasma prostanoids is fraught with the problem of interfering substances, we assessed plasma 6kPGF1 alpha and TxB2 levels in patients with SCD by RIA after extraction of eicosanoids and separation by HPLC. We demonstrate that the 6kPGF1 alpha and TxB2 levels in children with SCD in steady state as well as in vaso-occlusive crisis (VOC) are significantly lower when compared with those from age-matched controls. The VOC plasma 6kPGF1 alpha and TxB2 levels were, however, significantly elevated when compared with those from children in steady state. Changes similar to those noted with unpaired plasma samples were also observed when paired steady state and VOC plasmas from the same patients were assessed. The ratio of TxB2 to 6kPGF1 alpha was, however, significantly elevated in patients with SCD in crisis when compared with eicosanoid ratios obtained during steady state. In an attempt to understand whether the abnormality in 6kPGF1 alpha was due to an impairment in endothelial cell prostacyclin-regenerating ability, we compared the ability of plasma from controls and children with SCD to activate arachidonic acid (AA) release and prostacyclin production by [14C]AA-prelabeled bovine aortic endothelial cells. Our results suggest that the decreased 6kPGF1 alpha levels in plasma from children with SCD was not due to an effect on substrate AA release but rather a modulatory effect of sickle plasma components on endothelial cell cyclooxygenase activity.(ABSTRACT TRUNCATED AT 250 WORDS)

6-Ketoprostaglandin F1 alpha↗

Participation in sports after hip and knee arthroplasty: review of literature and survey of surgeon preferences.

OBJECTIVE: To summarize previously published findings and to present the opinions of a group of reconstructive orthopedic surgeons from a single institution on participation in sports after hip or knee arthroplasty. DESIGN: We reviewed the literature pertaining to participation in sports after hip or knee arthroplasty and surveyed a group of orthopedic surgeons about their recommendations for resumption of various sports activities by patients who had undergone total hip or knee arthroplasty. MATERIAL AND METHODS: A computerized literature search was performed, and salient issues about participation in sports after joint replacement procedures were synthesized. At the Mayo Clinic, 28 orthopedic surgeons (13 consultants and 15 fellows or residents) completed a single-page questionnaire that requested a recommendation ("yes," "no," or "depends") about patients resuming participation in 28 common sports after recovery from total hip or knee arthroplasty. Staff surgeon responses were compared with responses from fellows and residents by using the Mann-Whitney U test. Sports in which 75% of surgeons would not allow participation were identified as "not recommended," whereas sports in which 75% of surgeons would allow participation were labeled as "recommended." RESULTS: Fellows and residents were less likely than staff surgeons to allow return to cross-country skiing after total knee arthroplasty. Otherwise, responses from consultant surgeons and from fellows and residents did not differ significantly. Recommended sports included sailing, swimming laps, scuba diving, cycling, golfing, and bowling after hip and knee replacement procedures and also cross-country skiing after knee arthroplasty. Sports not recommended after hip or knee arthroplasty were running, waterskiing, football, baseball, basketball, hockey, handball, karate, soccer, and racquetball. CONCLUSION: After hip or knee arthroplasty, participation in no-impact or low-impact sports can be encouraged, but participation in high-impact sports should be prohibited.

Hip Prosthesis↗

Injuries in youth ice hockey: a pilot surveillance strategy.

OBJECTIVE: To determine the incidence and type of injuries in youth hockey players during practice and games as well as to test the applicability of the current methods to a national surveillance study of youth hockey injuries. DESIGN: During the 1993 to 1994 season, we undertook a prospective observational analysis of youth hockey players on four competitive teams at three levels of participation: Squirt, Peewee, and Bantam. MATERIAL AND METHODS: In a study group of 66 youth hockey players, types and anatomic sites of injury, mechanisms of injury, player position affected, and occurrence of injuries during practice or games were determined and analyzed statistically. An injury was strictly defined, standardized reporting strategies were used, and a single physician examined all injured athletes and made the diagnoses. RESULTS: A total of 14 injuries occurred during the season. The on-ice injury rate (per 1,000 player-hours) was 1.0 at the Squirt level (ages 9 and 10 years), 1.8 at the Peewee level (ages 11 and 12 years), and 4.3 at the Bantam level (ages 13 and 14 years). No game injuries and only one mild practice injury occurred in Squirt players (N = 16), and only two mild injuries occurred during practice sessions for Peewees (N = 17). No differences were noted between practice injury rates at the various levels; all recorded game injuries occurred only in Bantam players (N = 33). The most common types of injuries were contusions, fractures, strains, and sprains. The arm and shoulder were most frequently injured. CONCLUSION: Further research is necessary to determine whether injuries in youth ice hockey can be reduced by changes in playing rules, enforcement of existing rules, improvements in protective equipment, alteration in coaching techniques, and institution of educational programs.

Adolescent↗

Medical coverage for multievent sports competition: a comprehensive analysis of injuries in the 1994 Star of the North Summer Games.

OBJECTIVE: To determine the incidence and type of requests for medical assistance and the treatment required in 21 sports at the 1994 Star of the North Summer Games and to develop a plan, based on these findings, for efficient allocation of medical supplies and staff. DESIGN: We reviewed our experience with a large multisport amateur athletic event and analyzed the pattern of injuries for participants in the various sports. MATERIAL AND METHODS: Injury evaluation forms and medical supply kits were given to the health-care volunteers, who received preliminary instructions on classifications and definitions of injuries and on appropriate completion of the forms. When a medical contact occurred, an evaluation form was completed. The data from these forms were subsequently compiled and analyzed. RESULTS: Of 6,243 athletes who participated in the 1994 Star of the North Summer Games, 55 (0.88%) received medical attention (2 officials also required medical assistance). The sports with the greatest number of medical contacts were soccer and track and field--31 and 16 contacts for medical assistance, respectively. The lower extremities were the anatomic site most frequently involved in injury (62% of the medical contacts). The most common types of injury were contusions, strains, and sprains. CONCLUSION: Because few injuries were sustained and most were of minor severity, basic medical supplies (such as ice bags and compression wraps) were sufficient. For locations with a scarcity of medical personnel, a triage system can be established in which athletic trainers or registered nurses manage the initial assessments and refer cases, as needed, to physicians for specialized care. An effective communication system can also considerably reduce volunteer hours.

Adolescent↗

In-line skating injuries.

OBJECTIVE: To assess the type and site of injuries associated with in-line skating in patients encountered during an 18-month period. DESIGN: We retrospectively reviewed the medical records of patients in our emergency department and Sports Medicine Center with injuries that had resulted from in-line skating between July 1992 and December 1993. MATERIAL AND METHODS: A computer search was conducted to identify medical records on which in-line skating was denoted as the mechanism of injury. The records were then manually reviewed to confirm that the injury had occurred during in-line skating. The patient's sex, age, type of injury, and injured body part were recorded. RESULTS: During the designated study period, 32 in-line skating injuries were recorded in 32 patients (19 female and 13 male skaters). The mean age of the study group of injured skaters was 17 years (range, 6 to 46). An upper extremity was involved in 78% of all injuries, and the wrist was the body part most often injured (56%). A lower extremity was involved in 16% of all injuries, including two that were severe. Of the 32 injuries, 62% were fractures, and surgical treatment was necessary for only 1 injury during the study period. CONCLUSION: In this study, in-line skating injuries most commonly involved the upper extremities. Fractures, particularly of the distal radius, were the most common type of injury. Lower extremity trauma was less frequent, but severe injuries can occur. Further prospective studies are needed.

Adolescent↗

Arachidonic acid metabolites are involved in mediating red blood cell adherence to endothelium.

As an initial investigation into the possible role of endothelial cell (EC) lipoxygenase and cyclooxygenase metabolites in the adherence of red blood cells (RBCs) to ECs, we evaluated the effect of nordihydroguaiaretic acid, (NDGA; 10 mumols/L, BW755c (30 mumols/L), aspirin (100 mumols/L), and indomethacin (10 mumols/L) on RBC-EC adherence using a static incubation system and 51Cr-labeled RBCs. NDGA and 3-amino-L-[3'-(trifluoromethyl)phenyl]-2-pyrazoline inhibitors of both the lipoxygenase and cyclooxygenase pathways, significantly decreased basal adhesion of RBCs to fetal bovine aortic ECs, whereas aspirin and indomethacin, selective inhibitors of the cyclooxygenase pathway, stimulated the adherence process. The inhibitor effect appeared to be mediated via an effect on EC functions, since preincubation of ECs with NDGA, in contrast to RBC-NDGA preincubation, inhibited the adherence process. Because bovine aortic ECs generate mainly prostacyclin and 15-HETE from arachidonic acid (AA) via the cyclooxygenase and the lipoxygenase pathways respectively, the role of these products (100 pmol/L to 1 mumol/L) on the adhesive process was further assessed. 15-HETE potentiated basal adhesion of RBCs to bovine aortic ECs in a concentration-dependent manner, with maximal responses of approximately 50% to 150% over baseline noted at concentrations between 1 and 100 nmol/L 12-HETE, a structural isomer of 15-HETE and the major platelet lipoxygenase product, also stimulated RBC adherence. In contrast, prostacyclin (assessed using carbacyclin, a stable synthetic analog of prostacyclin with similar biologic properties) had no significant effect on this process. In further studies, we demonstrated that the 12-HETE-induced adherence of sickle RBCs was mediated via an up-regulation of the vitronectin receptor on bovine aortic endothelium. Because microvascular capillary endothelium is the surface most likely to encounter erythrocytes in vivo, we extended our studies to human retinal capillary ECs to assess the involvement of eicosanoids in sickle RBC-microvessel adhesion. As with bovine aortic ECs, aspirin stimulated and NDGA decreased the adherence of sickle RBCs to human retinal capillary endothelium. These microvascular ECs generated prostacyclin, HHT, 15-HETE, and 15-HPETE from endogenous AA. Although carbacyclin and HHT had no effect on the adherence process, both 15-HETE and 15-HPETE (10 pmol/L to 100 nmol/L) stimulated RBC adhesion to capillary endothelium. Our studies document a role for the lipoxygenase metabolites in modulating basal adhesion of RBCs to both macrovascular and microvascular endothelium; the major cyclooxygenase metabolites appear to play no role in this process.

Animals↗