Biomedical subjects

M J Roth

Publications and source records attributed to M J Roth.

75 records · Page 5Linked to original sources

PubMedDate not supplied

Expression of cyclooxygenase-2 in human adenocarcinomas of the gastric cardia and corpus.

Several studies indicate that the use of non-steroidal anti-inflammatory drugs (NSAIDs) may reduce the risk of gastric corpus and possibly gastric cardia cancers. The best known action of NSAIDs is to block the enzyme cyclooxygenase, the rate limiting enzyme in the conversion of arachidonic acid to prostaglandins. We investigated the expression of cyclooxygenase-2 (Cox-2) in adenocarcinomas of the gastric cardia (N=19) and corpus (N=15) and in adjacent normal epithelium from a high risk Chinese population. Immunohistochemical detection of Cox-2 revealed positive staining in 36% of the gastric cardia cancer cases and 60% of the gastric corpus cancer cases, whereas histologically normal tissue from the same patients were negative. Smooth muscle, stroma and inflammatory cells were also positive. Our results suggest that Cox-2 is overexpressed in a large proportion of adenocarcinomas of the gastric corpus and in a smaller number of gastric cardia cancer cases.

Adenocarcinoma↗

PubMedDate not supplied

Expression of cyclooxygenase-2 in human squamous cell carcinoma of the esophagus; an immunohistochemical survey.

Several studies indicate that the use of non-steroidal anti-inflammatory drugs (NSAIDs) may reduce the risk of esophageal cancer. The best known function of NSAIDs action is to block the enzyme cyclooxygenase, the rate limiting enzyme in the conversion of arachidonic acid to prostaglandins. In this study we investigated the expression of cyclooxygenase-2 (Cox-2) in squamous cell cancers of the esophagus and in normal esophageal squamous epithelium. Immunohistochemical detection of Cox-2 revealed strong positive staining in the well-differentiated regions of esophageal tumors, whereas histologically normal squamous epithelium stained only weakly positive. Smooth muscle cells, some stromal and inflammatory cells were also positive. Poorly differentiated areas of the esophageal tumors were negative. Our results suggest that Cox-2 is over-expressed in well-differentiated regions of squamous cell cancers of the esophagus.

Adolescent↗

PubMedDate not supplied

Detection of the von Hippel-Lindau gene deletion in cytologic specimens using microdissection and the polymerase chain reaction.

Previous studies have demonstrated von Hippel-Lindau (VHL) gene abnormalities in renal cell carcinoma. Archival cytologic samples of renal cell carcinomas (RCCs) from two patients were evaluated for allelic loss of the VHL gene. Small clusters of renal carcinoma cells were dissected from cytocentrifuge preparations under direct microscopic visualization followed by single-step DNA extraction and subsequent polymerase chain reaction (PCR). Amplification of DNA from the harvested tumor cells demonstrated a loss of heterozygosity at the VHL gene in five RCC specimens obtained from two patients. The combination of microdissection and PCR enabled DNA studies to be performed on a pure population of tumor cells isolated from heterogeneous samples containing admixed normal cellular elements. Selected genetic studies performed on microscopically targeted cells will help to further the biologic and cytomorphologic characterization of the targeted cell population.

Alleles↗