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Biomedical subjects

M J Reid

Publications and source records attributed to M J Reid.

47 records · Page 3Linked to original sources
PubMed1981

Microtiter solid-phase radioimmunoassay for specific immunoglobulin E.

A microtiter solid phase-radioimmunoassay (MSPRIA) for perennial rye grass-specific IgE is described. The assay is carried out in flexible polyvinyl chloride "u" microtiter plates by sequentially incubating antigen, albumin, test sera, and finally radiolabeled antihuman IgE. Wells are cut free of the plate with a hot wire-cutting apparatus and counted individually in a gamma counter. The amount of radioactivity bound is proportional to the amount of specific IgE in the serum. The MSPRIA is shown to be antigen and antibody specific, reproducible, and easily done. Rye-specific IgE levels assayed with the MSPRIA correlated with quantitative end point titration skin testing using perennial rye grass antigen performed on 31 volunteers with a maximum correlation coefficient of 0.92. The MSPRIA was compared with the radioallergosorbent test (RAST) method using sera from the same 31 volunteers. The rye-specific IgE levels assayed by MSPRIA correlated with those assayed by RAST with a correlation coefficient of 0.95. The MSPRIA is well suited for mass screening and represents a useful method for measuring specific IgE.

Adult↗
PubMed1981

A microtiter solid phase radioimmunoassay for total serum IgE.

A two-step microtiter solid phase radioimmunoassay (MSPRIA) for total serum IgE has been developed by the authors. Microtiter wells are coated with rabbit antihuman IgE. During the first incubation. IgE in serum samples or the standard is bound to the antibody coated wells. The wells are then overlaid with radiolabeled goat anti-IgE antibodies. The amount of radioactivity bound is proportional to the amount of IgE in the test serum. The mean IgE level of 25 cord sera was found by this assay to be 0.3 U/ml. The total serum IgE's of 40 volunteers ranged from 4 to 593 U/cc. These values agreed with the values determined by commercial PRIST assay with a correlation coefficient of 0.98. The MSPRIA is a specific, sensitive, convenient and economical means of quantitating total serum IgE.

Animals↗
PubMedDate not supplied

What are the factors that influence learning in relation to nursing practice?

Although ongoing education is an expectation of all nurses in today's climate of increasing technology and patient acuity, little is known about the attitudes, beliefs, and values of the general staff nurse toward on-the-job learning. The authors detail the factors that influence the learning ability of nurses on general medical-surgical units in a teaching hospital in relation to their nursing practice. Findings of what promotes learning, what interferes with learning, and how learning is integrated into nursing practice are outlined. Implications for the future are discussed.

Education, Nursing, Continuing↗
PubMedDate not supplied

Mountain cedar pollinosis: can it occur in non-atopics?

In 1984 Ramirez postulated the existance of two subgroups of patients with Mountain Cedar (MC) pollinosis. One subgroup had a single positive skin test (SPST) to MC only, lacked other atopic diseases, and required prolonged MC exposure to develop the disease. The second subgroup had multiple positive skin tests (MPST) in addition to MC, had other atopic diseases, and developed clinical symptoms after a shorter period of MC exposure. To validate these findings, and to explore the clinical and immunologic differences between these two subgroups, 13 SPST and nine MPST patients underwent immunotherapy with MC pollen extract. Six SPST and ten non-allergic controls did not receive immunotherapy. MC specific IgE (sIgE), MC sIgG, and MC sIgG subclasses were measured by ELISA pre and intra season. Symptom Medication Score (SMS) were measured during the MC season. SPST patients had a significantly lower baseline sIgE than MPST patients, 2.1 IU/ml versus 22.3 IU/ml, p = 0.023, and were also older than MPST patients, 52.4 versus 32.2 years, p less than 0.001. Baseline MC sIgG and MC sIgG subclass antibody levels were similar in both patient groups. SMS were lower in treated SPST patients compared to treated MPST patients, p less than 0.01, but in vitro responses to immunotherapy were not significantly different between the two groups. MC sIgE, MC sIgG, MC sIgG1 and MC sIgG4 rose in both treated groups. MC sIgG1 (but not MC sIgG4) rose during the MC season in both non-immunotherapy groups.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗