Search PubMed⌕ Search

Biomedical subjects

M J Price

Publications and source records attributed to M J Price.

At least 37 records · Page 2Linked to original sources
PubMed1997

Thermoregulatory responses of paraplegic and able-bodied athletes at rest and during prolonged upper body exercise and passive recovery.

The thermoregulatory responses of ten paraplegic (PA; T3/4-L4) and nine able-bodied (AB) upper body trained athletes were examined at rest and during prolonged arm-cranking exercise and passive recovery. Exercise was performed for 90 min at 80% peak heart rate, and at 21.5 (1.7) degrees C and 47.0 (7.8)% relative humidity on a Monark cycle ergometer (Ergomedic 814E) adapted for arm exercise. Mean peak oxygen uptake values for the PA and AB athlete groups were 2.12 (0.41) min(-1) and 3.19 (0.38) l x min(-1), respectively (P<0.05). At rest, there was no difference in aural temperature between groups [36.2 (0.4) degrees C for both groups]. However, upper body skin temperatures for the PA athletes were approximately 1.0 degrees C warmer than for the AB athletes, whereas lower body skin temperatures were cooler than those for the AB athletes (1.3 degrees C and 2.7 degrees C for the thigh and calf, respectively). Upper and lower body skin temperatures for the AB athletes were similar. During exercise, blood lactate peaked after 15 min of exercise for both groups [3.33 (1.26) mmol x l(-1) and 4.30 (1.03) mmol x l(-1) for the PA and AB athletes, respectively, P<0.05] and decreased throughout the remainder of the exercise period. Aural temperature increased by 0.7 (0.5) degrees C and 0.6 (0.4) degrees C for the AB and PA athletes, respectively. Calf skin temperature for the PA athletes increased during exercise by 1.4 (2.8) degrees C (P<0.05), whereas a decrease of 0.8 (2.0) degrees C (P<0.05) was observed for the AB athletes. During the first 20 min of recovery from exercise, the calf skin temperature of the AB athletes decreased further [-2.6 (1.3) degrees C; P<0.05]. Weight losses and changes in plasma volume were similar for both groups [0.7 (0.5) kg and 0.7 (0.4) kg; 5.4 (4.9)% and 9.7 (6.2)% for the PA and AB athletes, respectively]. In conclusion, the results of this study suggest that the PA athletes exhibit different thermoregulatory responses at rest and during exercise and passive recovery to those of upper body trained AB athletes. Despite this, during 90 min of arm-crank exercise in a cool environment, the PA athletes appeared to be at no greater thermal risk than the AB athletes.

Adult↗
PubMed1997

Determination of peak oxygen uptake during upper body exercise.

The purpose of this study was to determine (1) the repeatability of a currently recommended protocol for determination of VO2 peak during upper body exercise involving a crank rate of 60 rev min-1 (Hale et al. 1988), (2) whether employing a higher crank rate (70 rev min-1) elicits higher VO2 peak values; (3) whether including an additional exercise stage 5 min after volitional exhaustion would elicit higher VO2 peak values. Twenty subjects (mean +/- SD) age, height and body mass were 22.8 +/- 1.8 years, 176 +/- 5.0 cm and 77.5 +/- 4.0 kg, respectively) visited the laboratory on three separate occasions. Subjects performed a different test to determine upper body VO2 peak on each visit. The tests were continuous and incremental in nature and were performed on a Monark cycle ergometer (Model 814E) adapted for arm exercise. Test 1 required subjects to exercise at a crank rate of 60 rev min-1 (60RPM-1). Test 2 was identical to test 1 (60RPM-2) but after 5 min recovery from the test subjects performed a further exercise stage (60RPM-F). Test 3 employed a crank rate of 70 rev min-1 (70RPM). Five minutes after volitional exhaustion a further exercise stage was performed (70RPM-F). The levels of agreement for the 60 rev min-1 protocol were +/-0.50 1 min-1. All VO2 peak values achieved were within these limits. However, a higher VO2 peak was achieved by employing a crank rate of 70 rev min-1 (p < 0.05). The VO2 peak achieved for 60RPM-F was similar to 60RPM whereas the VO2 peak for 70RPM-F tended to be less than that for 70RPM (p = 0.064). The results of this study suggest that the 60 rev min-1 protocol can reliably determine VO2 peak, however it would appear to be more appropriate to employ a 70 rev min-1 protocol as higher values were attained.

Adult↗
PubMed1995

Effect of nicotinamide and pentoxifylline on normal tissue and FSA tumor oxygenation.

Nicotinamide (NA) and pentoxifylline (PTX) sensitize experimental murine tumors to radiation without sensitizing normal tissues. They are presumed to exert this effect by reducing hypoxia in tumors. The present study evaluated the individual and combined effects of NA and PTX on oxygen levels in subcutaneous normal tissue and subcutaneous FSa fibrosarcoma tumors in the hind foot dorsum of C3H mice. Oxygen measurements were made using a polarographic needle electrode inserted into the tissue immediately before and/or 15-60 min after intraperitoneal administration of 500 mg/kg of NA, 50 mg/kg of PTX, or saline. The median tumor pO2 increased from a mean +/- S.E.M. of 4.1 +/- 1.1 mm Hg in saline-treated control mice to 6.8 +/- 1.9 mm Hg 15 min after NA, 7.6 +/- 1.4 mm Hg 60 min after PTX, and 6.7 +/- 1.1 mm Hg after NA and PTX in combination. PTX raised the median tumor pO2 level from 21% to 39% of the median subcutaneous normal tissue pO2 (p < 0.01). PTX also significantly reduced the proportion of tumor pO2 values < or = 2 mm Hg from 41 +/- 10% to 8 +/- 7% (p = 0.02). Although NA did increase the proportion of tumor that was well oxygenated, it did not significantly reduce the proportion of tumor pO2 values < or = 2 mm Hg (p = 0.34). The combination of NA and PTX did not add to the tumor oxygenation enhancement achieved by PTX alone. NA increased the median subcutaneous normal tissue pO2 by an average of 5.1 +/- 2.2 mm Hg from a baseline of 17.1 +/- 2.2 mm Hg (p = 0.04). PTX had no effect on the median normal tissue pO2 (p = 0.93). PTX showed greater therapeutic potential in this model system than did NA.

Analysis of Variance↗
PubMed1993

Exploration of body listening: health and physical self-awareness in chronic illness.

This phenomenologic study explored how healthy and chronically ill adults understand their body experience. Nine healthy adults and nine chronically ill adults (asthma or multiple sclerosis) were interviewed and asked to keep a 6-week diary. Using Colaizzi's method of data analysis, significant statements from all three groups were collated and analyzed for formulated meanings. The resultant body paradigm is defined as the person's explanatory model of who she or he is physically and is the result of past body experiences, shared body experiences of others while growing up, perceived circadian rhythms and personal gnosis. Perception of energy patterns was central to all three groups, and chronically ill informants described adapting to a new body paradigm.

Adaptation, Physiological↗
PubMed1993

An experiential model of learning diabetes self-management.

The literature on self-management in diabetes consists predominantly of investigations on compliance, behavioral modification techniques, assessment of health beliefs, and cognitive theories. Little systematic research has explored the actual experience of applying and adapting to a diabetes regimen. This qualitative study reports a diabetes self-management model (DSMM) based on interview data from 18 adults with Type I diabetes. As described and confirmed by the study participants, the DSMM is composed of sequential phases and stages that evolve over time and is influenced by four major factors: personal considerations, monitoring activities, specific cognitive skills for diabetes problem solving, and definition of control. This study provides a model that affirms the experience and effort of learning self-management and recognizes the importance of individualized regimens developed from personal experiences and perceptions of what "works for me."

Adult↗
PubMed1992

Purification and analysis of proteinase-resistant mutants of recombinant platelet-derived growth factor-BB exhibiting improved biological activity.

Recombinant platelet-derived growth factor (PDGF)-BB was expressed and secreted from yeast in order to study the structure-function relationships of this mitogen. A simple purification scheme has been developed which yields greater than 95% pure PDGF-BB. Analysis of this recombinant PDGF-BB shows partial proteolysis after arginine-32. Substitution of this arginine residue, or arginine-28 [a potential KEX2 (lysine-arginine endopeptidase) cleavage site], prevents or reduces cleavage of PDGF-BB respectively. These mutations result in a 5-fold increase in expression levels of PDGF-BB, and the resulting mutant proteins show higher activity in a number of biological assays than the cleaved wildtype PDGF-BB. These data are in accord with previous work by Giese, LaRochelle, May-Siroff, Robbins & Aaronson [(1990) Mol. Cell Biol. 10, 5496-5501] suggesting that the region isoleucine-25-phenylalanine-37 is involved in PDGF-receptor binding.

3T3 Cells↗
PubMed1992

Characterization of the structure and conformation of platelet-derived growth factor-BB (PDGF-BB) and proteinase-resistant mutants of PDGF-BB expressed in Saccharomyces cerevisiae.

A detailed biophysical study of the secondary and tertiary structures of recombinant platelet-derived growth factor (PDGF)-BB produced in yeast has been carried out. The secondary structure of the molecule is composed of 54% beta-sheet with less than 5% ordered helix. The single tryptophan residue has been shown to be solvent-accessible; however, the ability of the side chain to rotate is severely restricted. The fluorescence emission is quenched at pH 7.0 and in the presence of high salt, but dequenched by titration to lower pH with a pK of 5.8. Two proteinase-resistant mutants of PDGF [( Ser28]- and [Pro32]-PDGF-BB) have also been characterized and shown to have secondary and tertiary structures indistinguishable from wild-type PDGF-BB. These are, therefore, suitable stable background molecules in which to carry out structure-activity-relationship studies on PDGF-BB.

Mutagenesis, Site-Directed↗
PubMed1992

Abnormalities in color vision and contrast sensitivity in Parkinson's disease.

Dopamine is a neurotransmitter found in the retina. Delays in the visual evoked responses and abnormalities in contrast sensitivity occur in patients with Parkinson's disease. Improvement in the P100 has followed L-dopa therapy. Suspected abnormalities at the retinal level in Parkinson's disease are observed in reductions in photopic, scotopic, and pattern-derived electroretinograms. We studied 35 patients with Parkinson's disease and 26 controls of comparable age and visual acuities using visual evoked responses, color vision, and contrast sensitivity testing. Contrast sensitivity thresholds were significantly different at most frequencies tested, using both stationary and temporally modulated sinusoidal gratings. The total error score of the Farnsworth-Munsell 100 Hue Test revealed significant differences between the patients and controls. The contrast thresholds derived from certain spatial frequencies and the total error in color score were significantly related to the duration of disease. A stepwise discriminant analysis correctly identified 94% of the patients and 94% of the controls. The significant error in chromatic discrimination observed in Parkinson's disease patients may be due to altered intraretinal dopaminergic synaptic activity in these patients.

Color Perception↗
PubMed1992

Developing national guidelines for nurse practitioner education: an overview of the product and the process.

In 1988, the National Organization of Nurse Practitioner Faculties appointed an ad hoc education committee (AHEC) to review existing nurse practitioner (NP) education curricula and to formulate national guidelines. An overview of the new NP guidelines are presented along with the philosophy, content, objectives, and competencies that are viewed as the foundation for NP education today. The guidelines are presented within the structure outlined in Brykczynski's (1985) research about NP practice. Thus, as a "product" of the Advanced Nursing Practice: Nurse Practitioner Curriculum Guidelines (NPCG), a new NP graduate for the 1990s could be described as one who demonstrates beginning competencies in primary health care theory and research within the five domains of practice identified by Brykczynski.

Clinical Competence↗
PubMed1991

Secretion of human epidermal growth factor from Saccharomyces cerevisiae using synthetic leader sequences.

We have investigated different leader sequences for their ability to direct the efficient secretion of human epidermal growth factor (hEGF) from Saccharomyces cerevisiae. We designed a consensus signal sequence which directs secretion of hEGF from yeast as efficiently as the yeast invertase signal sequence. However, secretion is increased over fivefold by the introduction, after the signal sequence, of a synthetic 19-amino acid (aa) pro-sequence containing a cleavage recognition site for the KEX2 protease. Even in the absence of an Asn-linked glycosylation site, secretion of hEGF using the synthetic prepro-leader was as efficient as that directed by the alpha-factor leader. The role of the KEX2 protease cleavage site was investigated by mutation of the yeast alpha-factor KEX2 site (cleavage after Lys-Arg). Cleavage was obtained with the following order of efficiency, Lys-Arg greater than Pro-Arg greater than Asp-Arg, although the sequence context was also found to affect efficiency.

Amino Acid Sequence↗
PubMed1989

Ferrochelatase activity in Azospirillum brasilense with reference to the influence of metal cations.

Ferrochelatase in membrane preparations from Azospirillum brasilense displayed an activity of 2.17 mumol protoheme formed.h-1.mg protein-1 which is 10-fold greater than previous reports for other bacteria. This ferrochelatase showed an apparent Km of 20.9 microM for Fe2+, a pH optimum of 6.0-6.5, and stimulation by oleic or stearic acids. Co2+, Cu2+ and Zn2+ inhibited the incorporation of Fe2+ into protoporphyrin IX while Ni2+ and Mg2+ had no effect on protoheme synthesis. Activity with Fe2+ and mesoporphyrin IX was less than with protoporphyrin IX but deuteroporphyrin IX produced the highest rate of protoheme synthesis. The membrane fraction containing ferrochelatase activity was found to insert Cu2+, Ni2+, Zn2+ and Co2+ enzymatically into protoporphyrin IX to produce metalloporphyrins. Cu2+ incorporation into protoporphyrin IX proceeded at a rate greater than with Fe2+ and the Km for Cu2+ was 21.9 microM.

Azospirillum brasilense↗
PubMed1988

The pattern electroretinogram and visual-evoked potential in glaucoma.

The pattern electroretinogram (PERG) may reflect ganglion cell or inner retinal layer activity. The most sensitive spatial and temporal variables for testing patients with glaucoma have not yet been identified. Fifty-two glaucoma suspects, 51 glaucoma patients, and 28 normal subjects were studied with the PERG and VEP, using three repetition rates and three spatial frequencies. Fast Fourier transforms were calculated at each spatial frequency and reversal rate. An analysis of variance revealed that normals could be differentiated from ocular hypertension and glaucoma patients using the amplitude of the PERG (second and fourth harmonic). Abnormalities in phase of the PERG between groups were also detected. A discriminant analysis of all amplitude and phase data revealed that the phase shift of the response of the second harmonic at 11 alternations/s (15-min checks) and at 5.5 alternations/s (15-min checks) correctly identified 81% of the normal and 75% of the glaucoma patients. The phase shift determinations of the VEP revealed significant abnormalities using 2 and 1/2 standard deviation confidence limits. There was significant overlap in the pattern ERG amplitude and phase shift in all three groups.

Analysis of Variance↗
PubMed1988

Strategies and conditions for teaching theoretical nursing: an international perspective.

How, when, and why to teach nursing theory are still central questions for nurse educators. The questions are restated and generalized to the whys, and hows, and the whens to incorporate theoretical nursing rather than nursing theory in nursing education. Process and strategies for teaching theoretical nursing are compared and contrasted with those related to research. We propose the use of similar strategies and add several essential conditions related to theoretical nursing. In teaching theoretical nursing, educators are expected to develop an environment that values theory and ways by which a theoretical identity is facilitated and fostered in students. Critical and reflective thinking as well as the role modelling of local models of excellence are central ingredients in the development of nurses who are committed to using and developing theories. One approach in incorporating theory in curricula is to identify central components and goals such as historical analyses, domain boundaries, theory utilization, process and strategies for theory development and testing. Once components are identified, faculty can make a decision about where to incorporate them. We have proposed several approaches that incorporate theoretical nursing in undergraduate and graduate programmes.

Curriculum↗
PubMed1985

Pupillary constriction to darkness.

Patients with congenital achromatopsia and congenital stationary night blindness have been known to show a transient pupillary constriction to darkness. We examined 50 normal subjects and 108 patients with retinal and optic nerve dysfunction to see if any had an initial pupillary constriction to darkness. We used a new infrared television apparatus. Four patients with congenital stationary night blindness, four with achromatopsia, two with bilateral optic neuritis, and one with dominant optic atrophy showed the phenomenon. In the patients who showed this unusual pupillary response to darkness it was the first observable event every time the lights were turned off. The constriction could usually be seen with a handlight, and it was similar in latency to the normal pupillary dilatation to darkness. Pupillary constriction to darkness is a clinically valuable sign that can be used in the detection of congenital retinal disease in children with poor vision.

Adolescent↗
PubMed1983

The pattern electroretinogram in optic nerve disease.

Pattern evoked electroretinograms (PERG), diffuse flash electroretinograms (ERG) and visual evoked potentials were studied in patients with unilateral optic nerve disease. Patients with Snellen acuities of less than 6/30 did not have recordable PERGs in their affected eye, whereas their diffuse flash ERGs were normal. The VEPs were correspondingly reduced or absent when recorded from the poorer seeing eyes. A second group of patients with Snellen acuity between 6/6 and 6/30 in the involved eye showed reductions in the mean PERG amplitude of the affected as compared with the normal eyes. All affected eyes showed an abnormal contrast threshold measured with the PERG amplitude. Such results underscore the diagnostic value of the PERG in detecting even mildly affected cases of optic nerve disease.

Adult↗