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Biomedical subjects

M J Hollenberg

Publications and source records attributed to M J Hollenberg.

At least 37 records · Page 2Linked to original sources
PubMed1976

Surface morphology of lens fibers from eyes of normal and microphthalmic (Browman) rats.

The surface features of cortical fibers from lenses of normal adult rats and microphthalmic rats of the Browman strain have been studied by scanning electron microscopy. In the normal lenses, superficial cortical fibers follow a straight course from inner to outer pole whereas the deeper cortical fibers, while straight near the poles, pursue an undulating or zig-zag course at and near the equator. Almost all of the fibers are hexagonal in cross section and all fibers throughout their entire length are bound by interdigitating processes at each corner of the hexagon to corners of two adjacent fibers. Some fibers are also affixed by a single row of ball and socket junctions located on their broad outer and inner surfaces. Lens fibers from Browman rats display both minor and major abnormalities. These included segmentation, formation of incisures and lateral protrusions, corrugation and villous-like alteration of the broad fiber surface and development of parallel ridges of broad surfaces in a basket-weave pattern.

Animals↗
PubMed1976

Lesions produced by human herpesviruses 1 and 2. Morphologic features in rabbit corneal epithelium.

The morphologic features of corneal lesions produced in rabbits by human herpesviruses, types 1 and 2 (HH1 and HH2), were studied using light and electron microscopic techniques. Also, healing lesions produced by the HH1 virus and treated by idoxuridine were similarly investigated. Scanning electron micrographs showed that although the morphology of HH1 and HH2 lesions was similar in most respects. HH2 lesions typically were raised, whereas the edges of HH1 lesions were not elevated above the corneal surface. In both HH1 and HH2 lesions, infected epithelial cells first separated from neighboring cells, then became globular, and finally were removed, leaving a central excavation. During healing of HH1 lesions, normal epithelial cells invaded the central crater from all sides to cover damaged cells and reconstitute the epithelial surface.

Animals↗
PubMed1976

Ultrastructural study of lesions induced in rabbit cornea by Herpes Simplex virus types 1 and 2.

Herpes simplex virus types 1 and 2 were used to infect rabbit corneas (without abrasion), and the resulting lesions were compared by light and electron microscopy. The type 1-induced lesion developed more quickly; the infected epithelial cells underwent a progression of defined morphological changes leading to organelle destruction, cell rounding, and death. Virus particles were abundant in the nuclei and cytoplasm of all types of cell in the epithelium, in intercellular spaces, and on the basement membrane. Occasionally, virus was seen to replicate within stromal fibroblasts. In contrast, type 2, although causing similar changes in the epithelial cells, was relatively inefficient in the production of mature virions. Thus type 2 nucleocapsids were synthesized within nuclei in numbers comparable to those of types 1, but intact cytoplasmic virions were uncommon, apparently because of degradation of type 2 virions in cytoplasmic vesicles or tubules. Extracellular type 2 particles were rarely seen. This finding offers an explanation for the relatively slow development of the type 2-induced lesion, but not for its greater severity. All epithelial cells appeared to be capable of supporting replication of either type of virus.

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