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Biomedical subjects

M J Haas

Publications and source records attributed to M J Haas.

43 records · Page 3Linked to original sources
PubMed1984

The effects of chronic metabolic acidosis on patterns of protein synthesis in rat renal cortex.

In the rat chronic metabolic acidosis increases the net synthesis of 17 renal cortex proteins by amounts ranging from 1.5 to 4.5-fold. These proteins have molecular weights between 13,000 and 42,000 and isoelectric points between approximately 5.5 and 7.0. No new proteins not also present in normal animals are detected in renal cortex samples from acidotic animals. Three proteins undergo substantial reductions in their net synthetic rates in chronic metabolic acidosis. On the basis of their physical properties and similar alterations in net synthetic rate in acidosis some of these proteins appear to be closely related and may be coordinately expressed in the rat kidney.

Acidosis↗
PubMed1984

Genetic Construction of Lactose-Utilizing Xanthomonas campestris.

Xanthomonas campestris, the producer of xanthan gum, possesses a beta-galactosidase of very low specific activity. Plasmid pGC9114 (RP1::Tn951), generated by the transposition of the lactose transposon Tn951 to RP1, was conjugally transferred into XN1, a nalidixic acid-resistant derivative of X. campestris NRRL B-1459S-4L. Transfer occurred on membrane filters and in broth. The beta-galactosidase gene of Tn951 was expressed in X. campestris. The specific activity of beta-galactosidase in transconjugants was over 200-fold higher than that in XN1, and transconjugants grew as well in lactose-based media as in glucose-based media. The lactose-utilizing transconjugants could potentially be used to produce xanthan gum from cheese whey.

Journal Article↗
PubMedDate not supplied

In vitro comparison of cefoperazone/sulbactam with selected antimicrobials against 300 bacteroides isolates. Inhibitory activity and time-kill kinetic studies.

The susceptibilities of 258 Bacteroides fragilis group isolates and 42 other Bacteroides species isolates against cefoperazone, cefoperazone/sulbactam (2:1 ratio) and selected other antimicrobials were determined by broth microdilution method. All isolates were susceptible to cefoperazone/sulbactam, ampicillin/sulbactam, ticarcillin/clavulanate, metronidazole, and imipenem. Other antibiotics showed variables levels of resistance (5-30%). Killing curves with the cef/sulb against selected B. fragilis group isolates were performed and showed excellent bactericidal activity at two to four times the minimum inhibitory concentration (MIC) after 12 hr incubation, even against the isolates with high cefoperazone MICs (greater than or equal to 64 micrograms ml). There was no regrowth at 24 hr. Cefoperazone/sulbactam is a compound with excellent inhibitory and bactericidal activity against B. fragilis group isolates.

Anti-Bacterial Agents↗