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Biomedical subjects

M J Corbel

Publications and source records attributed to M J Corbel.

At least 91 records · Page 5Linked to original sources
PubMed1983

Biochemical and pathological changes in experimental phycomycosis.

Intravenous inoculation of rabbits with spores of Absidia corymbifera strain V.73/8 produced acute phycomycosis and death within 2 to 10 days. Cultural and microscopical examination showed that fungal infection was widespread and involved most organ systems but with particularly extensive lesions developing in the kidneys. The progress of the infection was associated with a raised leucocyte count, an increasing erythrocyte sedimentation rate and significant changes in serum biochemistry. The latter included a decrease in serum iron, zinc, alkaline phosphatase and gamma-glutamyl transpeptidase concentrations but an increase in the synthesis of acute phase proteins and in the phenylalanine:tyrosine ratio and in serum concentrations of copper, magnesium, potassium, lactate dehydrogenase and triglycerides. The serum urea concentration increased substantially during the terminal phase of infection.

Alkaline Phosphatase↗
PubMed1983

Characterization of Yersinia enterocolitica strains isolated from cattle, sheep and pigs in the United Kingdom.

The properties of 48 cultures identified as Yersinia enterocolitica or Y. enterocolitica-like organisms, including Y. frederiksenii, Y. intermedia and Y. kristensenii, were examined. Of these, 39 were isolated from faeces of apparently healthy pigs, five from healthy cattle, one from an aborted bovine fetus, one from an aborted lamb and one from a lamb suffering from acute enteritis. Most isolates from healthy animals were of Y. enterocolitica biotype 1 or Y. intermedia and belonged to O serogroups not usually associated with disease in man or animals. The isolates from abortion or enteritis cases were of Y. enterocolitica biotypes 3, 4 and 5 and belonged to the pathogenic serogroups 0:5b and 0:2a, 2b, 3. No organisms of serogroup 0:9 were found.

Agglutination↗
PubMed1983

Response of the badger (Meles meles) to infection with Brucella abortus.

Three badgers exposed to conjunctival instillation of Brucella abortus strain 544 developed a mild subclinical infection accompanied by the production of agglutinating and complement fixing antibodies. Six months after inoculation, the infection could not be detected in one badger and was confined to lymphoid tissue in the other two. No cell mediated immune response to B abortus antigens could be detected by in vitro lymphocyte transformation tests or intradermal tests for delayed hypersensitivity during the observation period.

Animal Population Groups↗
PubMed1982

Characterization of the major antigens of Haemophilus equigenitalis (contagious equine metritis organism).

Immunoelectrophoresis of ultrasonically disrupted Haemophilus equigenitalis (contagious equine metritis organism) cells against rabbit and equine antisera disclosed at least 11 precipitating antigens. Two of these, a polysaccharide and a lipopolysaccharide-protein complex, were of high molecular weight and located on the cell surface. The remaining antigens were intracellular and were small- to medium-sized proteins. The surface antigens were the most significant in relation to the serological response in infected horses. They also reacted with sera from apparently healthy cattle, but the reason for this was not determined. No serological cross-reaction between H. equigenitalis and species of Achromobacter and Moraxella was detected.

Animals↗
PubMed1982

Experimental Brucella abortus infection in the horse: observations during the three months following inoculation.

Five mares, one stallion and a colt foal were inoculated intraconjunctivally with Brucella abortus strain 544. No clinical signs of disease developed except mild pyrexia. Intermittent bacteraemia was detected in the mares but not in the stallion or foal. Antibodies to B abortus became detectable from the second week after inoculation. Titres in the serum agglutination and complement fixation tests declined substantially after six to eight weeks but reactions to the Coombs antiglobulin, 2-mercaptoethanol and immunodiffusion tests were maintained. No consistent changes in biochemical or haematological values were observed. Post mortem examination of the foal disclosed granulomatous lesions in the lungs, liver, testes and metatarsophalangeal synovial membranes. B abortus identical with strain 544 was recovered from lymphoid and other tissues.

Animals↗
PubMed1982

Disc electrophoresis of phenol:acetic acid:water-soluble proteins for the identification of Yersinia species.

Disc electrophoresis of phenol:acetic acid:water extracts of Yersinia pestis, Y produced pseudotuberculosis, Y enterocolitica, Y philomiragia and Y ruckeri protein separation patterns characteristic of each species. The patterns produced by Y pestis, Y pseudotuberculosis, Y enterocolitica and Y ruckeri showed features in common. Those given by Y philomiragia strains were quite distinctive and showed little similarity to the other Yersinia species. All Y enterocolitica strains produced similar patterns irrespective of biochemical properties. Strains classified as Y intermedia or Y enterocolitica-like produced patterns indistinguishable from those given by Y enterocolitica.

Acetates↗
PubMed1981

Characterisation of Brucella abortus strain 19 cultures isolated from vaccinated cattle.

Thirty-four cultures recovered from material of bovine origin in England, Scotland and Wales were identified unequivocally as Brucella abortus strain 19 (S19). All had the properties of carbon dioxide-independent B abortus biotype 1 strains, were inhibited by penicillin G and thionin blue at standard concentrations and behaved in oxidative metabolism and guinea pig virulence tests as typical S19. Their sensitivity to i-erythritol varied somewhat between cultures as did reference subcultures of S19. Of the total number of isolates, 11 were recovered from abortion material or cyetic products, 10 were from calves which died from a hypersensitivity reaction within 24 hours of S19 vaccination and the remainder were from milk or internal organs. From the evidence available, there is little to suggest that calfhood vaccination with S19 has resulted in persistent systemic infection in other than a very small proportion of the animals inoculated.

Animals↗
PubMed1980

Examination of the relationship between pathological changes, immunological response and serum protein concentrations in pregnant sheep inoculated with Aspergillus fumigatus.

Pregnant sheep inoculated with Aspergillus fumigatus conidia developed precipitating and latex agglutinating antibodies to mycelial antigens. The titres of these tended to be higher in those animals developing placental or fetal infection than in those which did not. The concentrations of total serum proteins and of albumin, alpha, beta and gamma globulins did not show any consistent changes which could be related to abortion or placental infection. Lymphocyte transformation tests on whole blood showed significant responses to phytohaemagglutinin but not to A. fumigatus antigens.

Agglutinins↗
PubMed1980

An association between arthritis and persistent serological reactions to Brucella abortus in cattle from apparently brucellosis-free herds.

Twenty-four cases of stifle (femoro-tibial joint) arthritis, in Friesian heifers that had failed their first blood test for brucellosis, were examined pathologically and by serological and cultural tests for brucella. The arthritis was confined to the stifle and was non-purulent. The cases showed several other similar features, including evidence of trauma, erosions of articular surfaces, synovial proliferations and granulomata containing germinal centres and plasma cells. The synovial fluid was turbid, discoloured and usually increased in volume, with high antibody titres to brucella. Cultures were invariably negative for brucella, but examinations of cryostat sections stained by fluorescent antibody against B abortus were positive in five out of six cases examined some showing particles with a morphology resembling that of brucella.

Animals↗
PubMed1980

Isolation and properties of an RNA fraction present in Brucella culture supernatants.

The supernatant fluids of batch and continuous cultures of Brucella strains contained up to 100 mg/l of soluble RNA which could be recovered by precipitation with lysozyme, This RNA fraction had many of the properties of ribosomal RNA and was single-stranded, sensitive to ribonuclease, with an approximate sedimentation constant of 5S, a molecular weight of about 35000 daltons and an adenine; guanine; cytosine; uracil content of 17.5; 26.5; 33; 23 mol% respectively. RNA fractions from lysozyme precipitates evoked high titres of Brucella agglutinins on injection into rabbits and induced acute inflammatory responses in guinea-pig skin. Highly purified RNA fractions prepared by phenol extraction of lysozyme precipitates did not evoke antibodies to Brucella abortus.

Animals↗
PubMed1980

Properties of a cell-wall-defective variant of Brucella abortus of bovine origin.

The properties of an atypical Brucella strain isolated from lymph node tissue of a cow slaughtered as a brucellosis reactor were examined. The organism was Gram negative and highly pleomorphic, existing as cocci, coccobacilli, rods, branched and irregular forms which stained with fluorescent antibody conjugates prepared against rough and smooth Brucella abortus strains. It produced lecithinase and required at least 15% v/v equine or bovine serum for growth. It did not need supplementary CO2 for growth, produced H2S and was inhibited by brucella dyes and partially by i-erythritol. Growth inhibition or lysis was produced by brucella-phages. The organism was not pathogenic for guinea-pigs or mice but evoked antibodies mainly to rough Brucella antigens.

Animals↗
PubMed1980

Infection with Absidia corymbifera in bank voles (Clethrionomys glariolus).

Bank voles, Clethrionomys glariolus, kept under laboratory conditions were highly susceptible to both naturally-acquired and experimental infection with the phycomycete Absidia corymbifera. The infection was manifested as an acute lethal disease characterized by fungal invasion of the kidneys, central nervous system and, less frequently, other tissues. Natural infection was probably acquired via the respiratory route.

Acute Disease↗
PubMed1979

Experimental cerebral lesions produced by inoculation with Basidiobolus strains.

Two Basidiobolus strains produced progressive chronic granulomatous lesions in the brain following intracerebral inoculation of mice with hyphal material. Large septate hyphal bodies, together with sperical structures and granular material presumed to be of fungal origin, were present in the granuloma tissue surrounding the IIIrd and lateral ventricles. No progressive lesions developed when Basidiobolus mycelium was inoculated at extra-cerebral sites.

Animals↗
PubMed1979

Isolation and properties of a phage lytic for non-smooth Brucella organisms.

A phage for non-smooth cells of Brucella abortus was isolated from a mixture of three brucella-phages incubated with rough brucella cells in the presence of N-methyl-N'-nitro-N-nitrosoguanidine. It did not lyse smooth cells of Br. abortus strains nor those of other Brucella species, but during the course of its replication in rough organisms a small proportion of phage mutants were produced which were similar in properties to smooth-specific phages. The rough-specific phage, R, itself resembled Weybridge phage in its morphological and serological properties. Phage R was found to contain DNA and segregated into high and low density fractions, both with plaque-forming activity, on ultra-centrifugation in CsSO4 gradients. Unlike the smooth-specific phages it attached to heat-labile receptors present on non-smooth brucella cells.

Adsorption↗