Search PubMed⌕ Search

Biomedical subjects

M J Clarkson

Publications and source records attributed to M J Clarkson.

At least 55 records · Page 3Linked to original sources
PubMed1985

Ovine hydatidosis: some immunological characteristics of the seronegative host.

The immune status of a group of sheep naturally infected with hydatid cysts was investigated. Low specific anti-hydatid antibody (Sab) titres and negative results in intradermal and leucocyte migration tests occurred in hosts with high hydatid antigen-stimulated lymphocyte transformation (LT). Total (non-specific) IgG was elevated in infected sheep and was positively correlated with LT. Circulating hydatid antigen (cAg) was detectable in some infected sheep, but not in all of those with low Sab. Relationships between cAg, Sab and immune complex levels suggest that most cAg is present in immune complexes.

Animals↗
PubMed1985

Albendazole treatment of pulmonary hydatid cysts in naturally infected sheep: a study with relevance to the treatment of hydatid cysts in man.

Albendazole was given orally to sheep with naturally occurring live pulmonary and hepatic cysts. The viability of pulmonary cysts was established before treatment by thoracotomy and needle puncture. Both 10 and 20 mg/kg/day doses were found effective in that no viable protoscoleces were found after six weeks' treatment in either group while untreated controls still had viable cysts. In addition, treated animals showed macroscopic and electron microscopic changes. Bone marrow toxicity probably occurred in two sheep.

Albendazole↗
PubMed1983

Susceptibility of Chlamydia psittaci (ovis) to antimicrobial agents.

Minimum inhibitory (MIC) and minimal lethal (MLC) concentrations of 30 antimicrobial agents were determined for field strains and for the A22 EAE vaccine strain of Chlamydia psittaci (ovis). No differences in susceptibility could be detected between strains. The antimicrobials could be divided into four groups. Group A had no effect on growth and development, Group B were bacteriostatic at concentrations in excess of 100 mg/l, Group C were bacteriostatic at concentrations of 10 mg/l or less but were bactericidal at concentrations above 10 mg/l and Group D were bactericidal at concentrations above 0.5 mg/l. The value of antimicrobials in aiding diagnosis and in the therapy of chlamydial abortion of ewes is discussed.

Anti-Bacterial Agents↗
PubMed1982

Ovine enzootic abortion: the acquisition of infection and consequent abortion within a single lambing season.

The spread of enzootic abortion was observed in a flock of 37 unvaccinated Scottish blackface ewes, in mid-pregnancy at the start of the trial. Eight ewes were infected with the ZC 26 strain of chlamydia and allowed to run with 29 untreated controls until lambing was completed. All eight inoculated ewes aborted between 26 and 51 days after infection and chlamydial infection was demonstrated in seven of the abortions. Complement fixing antibodies were not detected in the controls until nine days after the first infected abortion, but within four weeks 69 per cent of the control ewes had developed significant complement fixation titres. Ten of the control ewes lost lambs, chlamydial infection was demonstrated in seven cases, two were undiagnosed and one was attributed to dystocia. The seven infected cases occurred between 69 and 87 days after the start of the trial and coincided with a further rise in the percentage of seropositive controls to a maximum of 90 per cent by the end of lambing.

Abortion, Veterinary↗
PubMed1981

Anthelmintic tests on Toxocara canis infection in mice.

One hundred and forty mice were infected orally with 1000 embryonated Toxocara canis eggs. Groups of 10 mice were then treated with high doses of piperazine, mebendazole, oxfendazole, albendazole, fenbendazole and diethylcarbamazine for four days, either immediately after infection or three weeks after infection. The mice were killed three to six weeks after treatment and the number of larvae in the brains and muscles estimated and compared with those in untreated mice. Few larvae were recovered from the muscles of any of the mice, including the untreated controls, despite the use of a variety of recognised techniques. Large numbers of live larvae were recovered from the brains of the mice and there was no significant difference between the treated and untreated mice.

Animals↗
PubMed1978

Hydatid disease in Wales: 2. Eradication.

The importance of hydatid disease in man and domesticated animals in Britain, and particularly in Wales, is reviewed. While the disease occurs throughout Wales the rural population in Powys shows the highest rate as estimated by hospital records and serological findings. The losses due to offal condemnation in sheep and cattle are estimated as at least 250,000. It is suggested that the feasibility of an area eradication scheme for Wales should be investigated; the form which this scheme might take is outlined.

Animals↗
PubMed1976

Measurement of liver blood flow by means of a single injection of bromosulphthalein, without hepatic venous catheterization.

1. A method is described for the measurement of liver blood flow without hepatic venous catheterization by the injection of the a single dose of bromosulphthalein and the analysis of the graph of the plasma disappearance of the indicator. 2. The rationale of the method was tested in three systems which covered a wide range of flow rates. The first system was a physical model with a haemodialyser in which the calculated flow could be compared with the flow measured directly. The second was in anesthetized dogs, in which the calculated flow was compared with that measured by continuous infusion and hepatic venous catheterization, based on the Fick principle. The third system was in unanaesthetized turkeys, in which metabolism of bromosulphthalein does not occur. The calculated flow was compared with the flow measured simultaneously by the uptake of collodial particles by the Kupffer cells. 3. In all three systems the correspondence between the two methods of measuring liver blood flow was good, the differences between them being not statistically significant. 4. The method has been applied to measurements of liver blood flow in man and animals and gives results comparable with those reported by other workers.

Animals↗