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Biomedical subjects

M J Cals

Publications and source records attributed to M J Cals.

31 records · Page 2Linked to original sources
PubMed1986

Fibronectin and retinyl acetate effects on attachment and spreading of normal and rheumatoid human synovial cells.

The action of two effectors - fibronectin (FN) and retinyl acetate (RA) - on cell attachment and spreading of human synoviocytes was investigated by adding these two drugs to the cell culture medium. No relationship was observed between the level of the effectors (FN = 20-80 micrograms/well, RA = 0.50-2 micrograms/well) and the biological effects studied. For normal human synoviocytes, fibronectin was less effective on the adhesion than fetal calf serum (FCS) present in the control culture medium; retinyl acetate, a drug acting on glycoprotein synthesis, led to similar effects to those observed for FCS-treated cells. In the case of rheumatoid synovial cells, the degree of adhesion was similar for drug- and FCS-treated cultures. Moreover, FN and RA had little effect on the spreading compared to FCS. Given these results, it would appear that synoviocytes differ in their behaviour from usual fibroblastic models.

Arthritis, Rheumatoid↗
PubMed1985

[Vitamin A augmentation of the effects of chemotherapy in metastatic breast cancers after menopause. Randomized trial in 100 patients].

Vitamin A was administered to randomly allocated patients in a group of 100 patients with metastatic breast carcinoma treated by chemotherapy. The daily doses (given indefinitely) ranged from 350,000 to 500,000 IU according to body weight. A significant increase in the complete response rate was observed. When subgroups determined by menopausal status were considered, it was observed that serum retinol levels were only significantly increased in the post-menopausal group on high dose Vitamin A. Response rates, duration of response and projected survival were only significantly increased in this subgroup. The therapeutic and biological implications of these findings are discussed.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1982

[Biological criteria of denutrition. Search of significant parameters for diagnosis and management in surgical departments].

Assessment of the nutritional status of surgical patients with a mean weight loss of 11.5 kg included determination of the following biochemical parameters: prealbumin, retinol-binding protein (RBP), serum vitamin A and serum zinc. Patients' immunological status was investigated by delayed hypersensitivity skin tests. Prealbumin and RBP are decreased in patients. RBP levels are significantly lower than in controls: RBP therefore seems to be a better criterion of denutrition than prealbumin. Serum RBP and vitamin A concentrations are not always correlated in patients; for instance, in malabsorptions, the sharp fall in serum vitamin A is isolated. The decrease in mean serum zinc concentrations is minor. Three patients have positive skin tests with severe biochemical disturbances; only serum zinc concentrations are normal and correlated with the results of skin tests. Interpretation of investigations in surgical patients is dependent on the cause of weight loss. However, biochemical and immunological assessment of the nutritional status can demonstrate specific deficits and improve preoperative nutrition.

Aged↗
PubMed1982

[Serum-ferritin assay in patients over seventy. A study on fifty-eight non anemic subjects and thirty-five anemic patients (author's transl)].

An immuno-enzymatic assay of serum ferritin was performed in subjects over 70 years of age. Wide variations in serum ferritin levels were demonstrated in the non-anemic group (17-510 ng/ml). This makes interpretation of results very difficult. In anemic patients a negative correlation was found between serum ferritin levels and TIBC (Total Iron Binding Capacity). When TIBC is under 42 mumol/l or above 63 mumol/l the two values are so closely correlated that serum ferritin assay does not provide any additional information for the diagnosis of anemia. On the contrary when TIBC is between 42 and 63 mumol/l serum ferritin can allow an estimation of tissue iron storage particularly in absolute iron deficiencies.

Aged↗
PubMed1982

[Usual values of serum vitamin A using a modified fluorimetric method].

We report a fluorimetric technique for the determination of serum vitamin A. The critical study of this micromethod included trials of repeatability, inter-series reproducibility and an evaluation of its accuracy. It was compared with a method using high performance liquid chromatography followed by detection with UV spectrophotometry. Normal values were established for adults. The variations according to age were determined for children between the ages of 10 months and 15 years.

Adolescent↗
PubMed1981

Fibroblast cultures in experimental disturbed metabolic conditions. effects of anti-inflammatory drugs.

Fibroblasts in normal metabolic conditions constitute a simple cellular model for the study of anti-inflammatory drugs in culture, but do not take into account the factors related to the acute inflammatory reaction. A new approach was tried in submitting the fibroblasts to the action of a mouse peritoneal exudate or selected activated macrophages. Disrupted peritoneal material was inoculated to cultures in a volume representing a number of cells corresponding to 25 or 50% of fibroblast concentration at day 0 of the experimental time. Three anti-inflammatory drugs were retained to study their effects on proliferation and metabolic parameters: hydrocortisone, indomethacin and a newly-described molecule, the methoxybenzalthiosemicarbazone (MBT). At day 0, 1, and 2, fibroblasts were counted and total cellular protein and cellular glycolytic activity were determined. 4.5 X 10(-5) M hydrocortisone and MBT protected the fibroblast from the antiproliferative action of peritoneal exudate whereas in experiments using activated macrophage homogenate, all drugs exerted a protective and stimulatory effect.

Animals↗
PubMedDate not supplied

[Vitamins and aging].

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Age Factors↗