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Biomedical subjects

M Izquierdo

Publications and source records attributed to M Izquierdo.

115 records · Page 7Linked to original sources
PubMed1975

DNA-RNA hybridization.

Interest in nucleic acid hybridization stems mainly from its great power as a tool in biological research. It is used in several quite distinct ways. Because of the high degree of specificity that they show, hybridization techniques can be used to measure the amount of one specific sequence within a very heterogeneous mixture of sequences. Measurements of 1/10(6)-10(7) have been recorded. In extension of this, various properties of a specific sequence can often be studied. Secondly, because the kinetics of nucleic acid hybridization are quite well understood, it can be used to characterize both a pure sequence and a very complex mixture of sequences, like the genome of a vertebrate. Thirdly, again because of its specificity, it can be used to measure homologies between different populations of nucleic acids. Lastly, in conjunction with other techniques, it can be used as a basis for the fractionation of nucleic acid populations and the purification of specific sequences. Specific examples of these applications are given, with special reference to the organization of the genome in higher eukaryotes.

Animals↗
PubMedDate not supplied

[Pneumonitis secondary to the inhalation of isocyanates. An analysis of 2 cases].

We present two patients with diffuse interstitial lung disease attributed to two different forms of presentation of pneumonitis due to inhalation of isocyanates. The first case was acute and accidental and the second was due to longer workplace exposure. In both cases, clinical, radiological and lung function findings as well as results of bronchoalveolar lavage were similar to those of hypersensitivity pneumonitis triggered by organic agents.

Acute Disease↗
PubMedDate not supplied

[Gene therapy in neuro-oncology].

INTRODUCTION AND DEVELOPMENT: The concept of gene therapy implies the transfer of genetic material to a cell, a tissue or an organ with the aim to cure an illness or at least to improve the clinical status of a patient. The essential components of the therapy are: a vector able to enter efficiently and selectively the target cells; and the therapeutic gene competent to cure the pathology. In cancer therapies, retroviruses are preferentially used as vectors because they only infect dividing cells. Among the most popular and successful gene therapies against cancer we find the immunotherapy, the antiangiogenic strategies, the killer-suicide genes and RNA interference against specific oncogenes. CONCLUSIONS: Each one of these therapies have proven successful in cancer cell lines and in experimental animals where it has been shown a clear antitumoral effect in the absence of appreciable secondary effects. At present, the majority of these procedures are in clinical phases but none as yet is considered a therapeutic option.

Animals↗