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Biomedical subjects

M Iwasa

Publications and source records attributed to M Iwasa.

At least 109 records · Page 6Linked to original sources
PubMed1983

Binding sites of seminal acid phosphatase to Canavalia gladiata DC lectin.

Binding sites of seminal acid phosphatase (AcP) to Canavalia gladiata DC lectin (Can G) were examined. Complex of AcP and Can G produced in a test tube was solubilized with alpha-methyl-D-mannoside and was fractionated with Sephadex G-200. The elution pattern of AcP activity of the complex was similar to that of seminal plasma, suggesting that the reaction of AcP and Can G was reversible. Crossed immuno-affinoelectrophoresis with intermediate gel containing free Can G or immobilized one showed that AcP molecule had two or more binding sites per molecule to Can G. AcP activity of frozen sections of the prostate was inhibited by tartaric acid but not by Can G. However, Can G bound to AcP of the sections could be detected with FITC-labeled anti-Can G. These results indicate that the active site of AcP to Can G is not identical with that to the substrate.

Acid Phosphatase↗
PubMed1983

Isolation and purification of Rh0(D) antigen of human erythrocyte membrane and its serological property.

To elucidate immunochemical properties of Rh-Hr antigens, influence of various solubilizers on the activity, and isolation of the antigens were investigated. As a very sensitive method to detect Rh-Hr antigens, the agglutination inhibition test was recommended in which diluted antisera (1:400-1:1,600), papainized red cells and microtitrate plates were used. Extraction of D antigen with Brij 35 and Triton X-100 was effective, but SDS was not successful. However, D activity of SDS solubilized stroma was recovered by adding reductants. The activity was slightly demonstrated at the preparation from D negative red cells. The influence of the reductants was discussed. SDS solubilized stroma was fractionated with gel filtrations and activated thiol Sepharose 4B. The final preparation with D activity was proved to correspond with Band 3 which was not stained with PAS reagent. Deoxycholic acid solubilized stroma was fractionated with gel filtrations and affinity chromatography, resulting in the preparation corresponding to Band 3 with potent Rh-Hr blood type activities. The activities were detected without reductants and the preparation from D negative red cells had no D activity. Rh-Hr blood type activities other than D were observed in the same fraction.

Agglutination Tests↗
PubMed1983

Serological distinction of H antigen between saliva and red cell in forensic practice.

Rabbit antisera were prepared by immunization with O red cells or O.Se saliva. Anti-O red cells (anti-Hr) were diluted with O.Se saliva and each of diluents was adopted for elution method on blood or body fluid stains. Anti-Hr gave positive reactions only with red cell stains. On the other hand, anti-O.Se (anti-Hs) absorbed with O red cells kept activity reacting with latex coated with O.Se saliva. Anti-Hs reacted with various body fluid stains but not with red cell stain. It is considered that anti-Hr and -Hs are useful tools for individualization of blood or body fluid stains in forensic practice.

ABO Blood-Group System↗
PubMed1983

Immunochemical investigations of Rh0(D) activity detected in Band 3 of red cell membrane.

Stroma from D positive red cell was solubilized with 1% deoxycholic acid (DOC) and was applied onto Sepharose 4B and 6B, followed by fractionation with activated thiol Sepharose 4B. By this method Band 3 containing no carbohydrate was prepared. The Band 3 was incubated with human anti-D, then immune complex soluble in DOC solution was produced. This complex was not prepared from red cell components other than Band 3, nor from Band 3 from D negative red cell. The complex agglutinated papainized D positive red cells. Molecular weight of the complex was estimated to be 500,000 to 700,000 by the gel filtration method. The molecular weight increased according to the time of incubation. The complex gave a precipitation line in counter immunoelectrophoresis (IEP), a single peak in crossed IEP against anti-Band 3, and a line at gamma-globulin region in IEP against anti-human serum. SDS-polyacrylamide gel disc electrophoresis of the complex showed the pattern consisting of fragments of immunoglobulin G and Band 3 which was not stained with periodic acid Schiff reagent. These support our results that Rh-Hr blood type activities are located in Band 3 almost free of carbohydrate.

Chromatography, Gel↗
PubMed1983

A simple method to detect AB antigen in blood grouping of body fluid stains.

A modified mixed agglutination method is described for detecting AB antigen from body fluid stain. Saline extract of body fluid stain was added to A.Se saliva stain which was beforehand sensitized with anti-A. AB antigen reacting with the stain was detected with the usual mixed agglutination method using anti-B and B red cells.

ABO Blood-Group System↗
PubMed1983

[Nutritional assessment of patients with esophageal cancer. "Nutritional Assessment Index (NAI)" to estimate nutritional conditions in pre-and postoperative period].

We studied on 66 patients with esophageal cancer with preoperative enteral hyperalimentation by elemental diet, comparing with 64 patients without it, and the following results were obtained; Items such as TP, Alb, etc. in surviving patients, as well as those who died within 3 months, were worse immediately before operation than those at the time of admission when neither TPN nor ED was yet in use. In 35 of 66 patients, there were significant differences between the patients with or without postoperative complications, and who were died after surgery, in arm circumference (AC), triceps skinfold (TSF), arm muscle circumference (AMC), albumin (Alb), prealbumin (PA), retinol-binding protein (RBP) and PPD skin test. From the studies of about 60 items with the computer, the index as follow were obtained. Nutritional Assessment Index (NAI) = 2.64 AC + 0.6 PA + 3.76 RBP + 0.017 PPD - 53.8 Nutritional status of the patients was divided retrospectively broadly to three groups, good (NAI greater than or equal to 60), intermediate (60 greater than NAI greater than or equal to 40), and poor (40 greater than NAI) in preoperative period. The incidence of postoperative complications and mortality rates were reflected significantly in NAI. NAI would be useful to know prospectively the probability of all kinds of postoperative complications as well as estimating the nutritional assessment.

Esophageal Neoplasms↗
PubMed1982

Immunoelectrophoretic analyses of antigenic and human specific proteins of red cell membrane.

Rabbit antisera were raised for red cell stroma extracted with each of the following solutions; 5 mM phosphate buffer pH 8.0 (A antigen). 5 mM EDTA containing 5 mM 2-mercaptoethanol (B), 1/15 M phosphate buffered saline (C), 0.05% SDS (D), 50 mM Tris-HCl buffer pH 8.0 containing 1% SDS, 1 mM EDTA and 1% 2-mercaptoethanol (E) and 1% Triton X-100 (F). Crossed immunoelectrophoresis showed that E antigen produced relatively numerous precipitation lines, 7-9, against each of the antisera, suggesting that E solution was superior to the others for membrane solubilization. However, the Ouchterlony test demonstrated potent precipitin activity in anti-C, and the antiserum absorbed with monkey stroma which was extracted with F solution was proved to have strict human specificity. By adopting the antiserum to affinity chromatography, human specific antigens were isolated. The antigens gave four bands moving faster than Band 5 at SDS-polyacrylamide gel electrophoresis none of which was stained by Schiff's reagent. Using absorbed anti-C, human specificity of blood stains kept us to for two years could be identified.

Animals↗
PubMed1982

An esterase isozyme in non-hemoglobin proteins of cell lysate reacting with mushroom lectin.

Of 20 kinds of plant lectins examined, agglutinin activity of only mushroom lectin was inhibited with non-hemoglobin proteins of the red cell lysate. Chromatography of non-hemoglobin proteins on DEAE-cellulose and on hydroxylapatite resulted in isolating a protein which gave a single precipitation line with esterase activity in immunoelectrophoresis. SDS-polyacrylamide gel disc electrophoresis demonstrated that the protein consisted of two subunits molecular weight of which was approximately 100,000 each.

Agglutination↗
PubMed1982

An interaction between human seminal acid phosphatase and Canavalia gladiata DC lectin.

Interactions between human seminal acid phosphatase (AcP) and five kinds of lectins were studied. Seminal plasma was mixed with the lectins at various ratios. The mixtures were centrifuged and the supernatants were assayed for AcP activity. The activity was effectively reduced only with Canavalia gladiata DC (CG) lectin. CG lectin purified by Sephadex G-200 gel filtration was added to two fractions of AcP separated by Sephadex G-200 gel filtration. There was no difference in the rate of reduction of the two fractions. To isolate seminal AcP by affinity chromatography, CG lectin was coupled to agarose gel. AcP bound with the agarose gel could be eluted with alpha-methyl-D-mannoside. Specific activity of the purified AcP was elevated to as much as 54-fold.

Acid Phosphatase↗
PubMed1982

[Transposition of the great arteries associated with the straddling tricuspid valve diagnosed preoperatively by two-dimensional echocardiography: report of a case].

A case of transposition of the great arteries associated with the straddling tricuspid valve and the hypoplastic right ventricle was presented, who was diagnosed by two-dimensional echocardiography and finally confirmed by operation. The patient, a 2-year-old girl, had cyanosis, clubbed fingers, effort dyspnea and poor growth. The electrocardiogram revealed both atrial and left ventricular hypertrophy. In the M-mode echocardiogram, the interventricular septal echo was recorded at the level of the midportion of the tricuspid valve and posterior tricuspid leaflet opened behind the interventricular septum during diastole. Two-dimensional echocardiography demonstrated a small right ventricle with the tricuspid valve and its orifice which were straddling over the interventricular septum. Contrast echocardiography disclosed that in early diastole the bolus of contrast echoes passed through the tricuspid orifice into the right and left ventricles simultaneously. Left ventricular angiocardiography in the left anterior oblique projection revealed the negative diastolic silhouette of the tricuspid valve straddling the interventricular septum. Operative findings were as follows; the tricuspid orifice was normal in size, but the crest of the interventricular septum traversed the medial 1/3 of the orifice, the posterior 2/3 of the septal leaflet was supported by an anomalous papillary muscle originated from the left ventricular cavity adjacent to the posterior papillary muscle of the mitral valve and the posterior leaflet of the tricuspid valve was supported with the short chordae from the crest of the septum. The diagnosis of the straddling tricuspid valve was accurately made with the use of two-dimensional echocardiography.

Child, Preschool↗
PubMed1981

The specificity of anti-A agglutinins from immunized rabbits and plant lectins.

A+ and A- rabbits were immunized with human A red cells, saliva of A+ or A- rabbits or human A.Se. A- rabbits responded well against A.Se saliva and A cells and slightly against A+ saliva, whereas A+ rabbits produced high-titered antibody against A cells which was abolished by absorption with O cells. Agglutination inhibition tests showed that anti-A from A- rabbits immunized with A.Se or A cells was not inhibited with A+ saliva, and anti-A from rabbits immunized with A cells was not absorbed with A.Se saliva. Hydroxylapatite chromatography separated each of A.Se and A+ saliva into three fractions of which the 1st and 3rd fractions of A.Se and only the 3rd of A+ had A activity. The 3rd fraction of A.Se and A+ did not inhibit anti-A from any rabbits immunized but the 1st of A.Se inhibited anti-A from all rabbits except for A+ immunized with A cells. The 1st and 2nd fractions reacted slightly with anti-A from A- immunized with A+ and from A+ immunized with A cells. D. biflorus anti-A distinguished human A from rabbit one but Mushroom anti-A could not. These results suggested qualitative differences between human and rabbit saliva A antigens.

ABO Blood-Group System↗
PubMed1981

Human specific antigens and species difference in non-hemoglobin proteins of red cell lysate.

Non-hemoglobin proteins (NHPs) of 12 species of mammalian were effectively isolated from red cell lysates with CM-Sephadex chromatography. Polyacrylamide gel disc electrophoresis demonstrated that human and monkey NHPs had 13 and 11 bands, respectively, but guinea pig and swine two to three bands. Rabbit anti-human NHP reacted with monkey, bovine, canine and badger as well as human. Absorption of the anti-NHP with monkey NHP resulted in preparing human specific anti-NHP. The specific antiserum gave two bands locating near the origin in immunoelectrophoresis. Human specific antigens were prepared by affinity chromatography with the anti-NHP. The antigens were composed of three bands, two of which had slightly faster mobility than carbonic anhydrase and the other migrated fastly on disc electrophoresis. These bands were proved to have neither LDH nor esterase activity.

Animals↗
PubMed1981

[Two-dimensional echocardiographic findings of a right ventricular diverticulum: a case report (author's transl)].

A 11-month-old boy with a right ventricular diverticulum was reported. To our knowledge, this is the 18th case in the literature and the first case observed by two-dimensional echocardiography. Using our systematic two-dimensional echocardiographic approach, the three dimensional structure of this anomaly was reconstructed correctly. On the short-axis view of the ventricle, an abnormal septal echo was revealed in the right anterior region of the interventricular septum, forming an another cavity from the right ventricle, and was observed to have proper own motion by real-time two-dimensional echocardiography. From apical four chamber view, the abnormal cavity was detected on the right side of the small right ventricle. By contrast echocardiography, a contrast echo appears in the right ventricle and then in the abnormal cavity. The diagnosis was confirmed by cardiac catheterization, angiography and operation.

Echocardiography↗
PubMed1980

Application of precipitating activity of Canavalia lineata DC lectin for isolation of alpha 2-macroglobulin from human plasma.

To isolate alpha 2-macroglobulin from human plasma, complex of Canavalia lineata DC lectin and plasma was used as a starting material. The complex was solubilized with 0.08 M phosphate buffer pH 7.2 containing 0.9% NaCl and 0.02 M alpha-methyl-D-mannoside. The solution was applied onto a Bio gel A-5m column and eluted with solubilizing buffer. Of three peaks, only the second peak contained alpha 2-macroglobulin. After equilibrating with 0.06 M potassium phosphate buffer pH 6.8, the fraction was applied onto a hydroxylapatite column and eluted with the same buffer. The eluate was confirmed to be extremely pure alpha 2-macroglobulin; immunoelectrophoresis with anti-human serum produced a single precipitation line, and SDS disc electrophoresis gave a single band at alpha-globulin region. Few differences in carbohydrate composition between this preparation and those prepared by other methods were observed. By this method, about 45% of alpha 2-macroglobulin present in plasma was recovered.

Blood Protein Electrophoresis↗