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Biomedical subjects

M Iu Shchelkanov

Publications and source records attributed to M Iu Shchelkanov.

At least 19 recordsLinked to original sources
PubMed2006

[Molecular genetic characteristics of the Newcastle Sterna/Astrakhan/Z275/2001 virus isolated in Russia].

The genome of the NDV strain Sterna/Astrakhan/2755/2001 isolated from a wild bird of the Volga River delta in 2001 was completely sequenced. The phylogenetic analysis of the strain investigated and other NDV strains clearly demonstrated that Sterna/Astrakhan/2755/ 2001 belonged to the lineage 5b. Comparative analysis of molecular genetic markers of pathogenicity of strain Sterna/Astrakhan/2755/ 2001 allows its velogenic character to be suggested.

Animals↗
PubMed2006

[The role of ecovirological zoning in prediction of the influence of climatic changes on arbovirus habitats].

The paper contains methodical basis of ecovirological zoning in the analysis of the problem of interrelation between climatic changes and the activity of natural nidi of arboviral infection. The authors offer an original hieroglyphic system of cartographic signs of arboviruses and a map of prevalence of arboviruses in various landscape zones of Noth Eurasia (within the boundaries of the former USSR.) They analyze the three main cartographic approaches to ecovirological zoning of natural territorial complexes, which imply analysis of a ralation to a landscape, conjugated component analysis, and paleographic reconstruction analysis.

Arbovirus Infections↗
PubMedDate not supplied

[The molecular epidemiological characteristics of the HIV-1 variants circulating among injection drug addicts on the territory of the CIS Nations].

Different strains of HIV-1, circulating among drug addicts introducing drugs intravenously and detected on the territories of Russia, Ukraine and Belarus, have been characterized by the methods of the comparative analysis of genetic sequences of different variants of HIV (gene typing) and the study of the immunological properties of autoimmune sera (serotyping).

Amino Acid Sequence↗
PubMedDate not supplied

[Biological properties of primary HIV-1 isolates with varying serotype].

Russian HIV variants with common (rapid/high SI and slow/low NSI) and rare (slow/low SI and rapid/high NSI) phenotypes are described. SI variants demonstrate a higher p24 concentration level than serotype-independent NSI. The majority of SI variants belonging to A, B, and C serotypes were isolated from patients with mainly stage B3. At stages B2 and C2, when the majority of viruses are characterized by NSI phenotype, serotype D isolates are characterized only by SI phenotype. The spectrum of cell tropism was wide for all rapid/high strains and narrow for all slow/low ones.

Amino Acid Sequence↗
PubMedDate not supplied

[Serotypical profile of HIV infection in Moscow].

A total of 310 sera from HIV-1 infected patients detected in Moscow were serologically typed using 10 V3-imitating synthetic peptides. Subtype B virus is the most prevalent in Moscow (66.7%), serotype A/C ranks second (21.3%).

Amino Acid Sequence↗
PubMedDate not supplied

[ West Nile fever among residents of the Astrakhan region in 2002].

Thirty-three persons infected with West Nile fever were detected in 2002 in the Astrakhan Region; the diagnosis was confirmed serologically and the maximal number of the infected was registered in August, same year. The indices of the specific humoral immunity varied from 3.3% to 27.1%. A monitoring determined the highest infection risk among the residents of the Volga middle delta.

Antibodies, Viral↗
PubMedDate not supplied

[Clinico-immunologic monitoring of HIV-infected patients: comparative analysis of indicators, characterizing development of the disease].

The paper contains the results of a 15-year monitoring (since 1986 to 2001) of HIV-infected patients followed up at the clinic of the Institute of Immunology, Federal Department "Medbioextrem", Russia's Health Ministry. An analysis of a category of HIV-infected persons, who practiced the intravenous consumption of drugs since 1997 to 2001, revealed not only concomitant multi-virus infections (hepatitis C--68%, hepatitis B--48%, herpes 41%, fungal infections--66% and bacterial infections--46%) but also lesions (up to 70%) in the gastrointestinal tract and in the hepatic-cellular and hepatic-biliary systems. It was established that, when only immunological indices are taken account of in presence of multi-virus infections, it does not ensure an adequate evaluation of an HIV-patient condition. The below biochemical parameters must be necessarily taken into consideration: activity of alanine aminotransferase, aspartate aminotransferase and of alkaline phosphatase. The changes in the biochemical parameters were shown to be essentially higher in HIV patients with hepatitis C virus (HCV) even during the 2 initial follow-up years, when the immunological parameters are in the norm or even insignificantly below it. A comparative analysis of dynamic clinical, immunological and biochemical indices was made within the case study for the purpose of defining the prognostic criteria for HIV progression scenarios.

Disease Progression↗
PubMedDate not supplied

[Algorithm for predicting development of HIV infection].

Described in the paper are the results of a 15-year monitoring (1986-2001) of HIV patients at the Clinic of the Institute of Immunology, Federal Department "Medbioextreme", Russia's Health Ministry. The gathered experimental data, including the immunologic, biochemical and general-clinical parameters, were comprehensively analyzed. Optimal schemes of complex HIV monitoring were designed to ensure a comparative and dynamic study of different clinical-and-laboratory indices for the purpose of defining the HIV-induced pathogenesis specificity and adequate therapeutic approaches.

Algorithms↗
PubMedDate not supplied

[The efficiency of combined therapy of herpes virus infection in HIV infected patients].

The target of the case study was to investigate the efficiency of an alternative combined therapy scheme of herpes simplex infections versus the routine therapy by acyclovir or famvir as applicable to HIV-infected patients. leukinferon was shown to induce the antoherpetic acyclovir efficiency. The use of the latter concurrently with cycloferon for the treatment of infections provoked by herpes simple virus-1 (HSV-1) and HSV-2 in HIV-infected patients prolongs the remission period in case of the above opportunistic infections. The leukinferon anti-herpetic efficiency is, obviously, related with the phagocytosis stimulation and with its positive influence exerted on hemopoiesis. The combined therapy can be stated to be most effective in HIV, clinical stages B1 and 2.

AIDS-Related Opportunistic Infections↗
PubMedDate not supplied

[Ecology and evolution of influenza viruses in Russia (1979-2002)].

The research results on ecology and evolution of influenza A viruses, which has been conducted by the Center of Ecology and Evolution of influenza Viruses of Ivanovsky's Institute of Virology, Russian Academy of Medical Sciences, for more than 30 years, are summarized in the paper. A gene pool of influenza A viruses circulating in Russia's territory was defined. Foci of influenza A viruses were detected in natural biocenosis. Issues conditioned by the population interrelations of influenza viruses, i.e. between the populations of wild and home animals and the populations of people, are also under discussion.

Animals↗
PubMedDate not supplied

[Circulation of West Nile virus (Flaviviridae, Flavivirus) and some other arboviruses in the ecosystems of Volga delta, Volga-Akhtuba flood-lands and adjoining arid regions (2000-2002)].

Comprehensive virological, serological as well as genetic studies of the ecology of West Nile Virus (WNV) as well as of some other arboviruses were undertaken in different ecosystems in the territories of the Astrakhan Region and of the Kalmyk Republic. The main carriers (mosquitoes, ticks, birds and mammals) were defined as involved in the circulation of viruses within the natural and anthropogenic biocenosis. Phylogenetic examinations of isolated strains and samples, which were positive in RT-PCR, showed an absolute predominance of genotype I virus that was most closely related to American and Israeli strains. At the same time, epidemic strains had up to 6% of nucleotide differences versus the historic strains isolated in the same region 20-30 years ago. Besides, the circulation of genotype IV was discovered; it was characterized by a lower pathogenicity, which, possibly, ensures the shaping of a pronounced immune interlayer bearing no epidemic consequences. An analysis of the study results on the WNV ecology denotes the epicenter of the endemic territory located in the middle part of the Volga delta.

Animals↗
PubMedDate not supplied

[Detection of West Nile virus in birds in the territories of Baraba and Kulunda lowlands (West Siberian migration way) during summer-autumn of 2002].

West Nile Virus (WNV) was discovered in 3 species of birds collected in summer-autumn, 2002, in the South of Western Siberia. WNV was identified by ELISA and RT-PCR. Three of 5 dead rooks (Corvus frugilegus), which were found in the territory of the Kulunda plain, were WNV-infected. WNV RNA was detected in 2% of samples of internal organs of aquatics birds, i.e. teal (Anas crecca) and garganey (Anas querquedula), caught in the Chany Lake (Baraba plain). Nucleotide sequencing of the 300-472 aa fragment of WNV protein E gene showed the maximum level of homology with strain WNV/LEIV-Vlg99-27889, which was isolated from a patient in Volgograd (1999). A high homology level of nucleotide sequencing denotes the relationship between the WNV circulating in the Northern Caspian Region and in the South of Western Siberia.

Amino Acid Sequence↗
PubMedDate not supplied

[HIV-1 serotyping of V3-mimicking peptides circulating in the Republic of Tajikistan among intravenous drug users].

The paper summarizes the results of HIV-1 serotyping by using 56 positive sera collected in the territory of the Republic of Tajikistan (RT) from intravenous drug users (IVDU). It was made by solid-phase ELISA based on synthetic peptides, mimicking different variants of the apical epitope of the HIV-1 gp120 V3-loop. Two types of conjugates, those specific to human IgG and IgA, were used to detect the immune complexes. Serotypes, as determined according to IgG and IgA-based ELISA, coincided, however, the latter were proven to be more suitable for serotyping. There is a high level of HIV-1 serotype heterogeneity among IVDU in RT; altogether, 4 serotypes were identified, i.e. B (10%), B+A/C (18%), A/C (20%) and A/C+B (52%). The modern serotype HIV-1 diversity in RT resembles the epidemiologic situation in the territory of the former USSR as observed in the late 80-ies-early 90-ies of the last century.

Adolescent↗