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Biomedical subjects

M Ishiguro

Publications and source records attributed to M Ishiguro.

At least 217 records · Page 12Linked to original sources
PubMed1981

Delayed-type hypersensitivity as revealed on the footpads of mice to azobenzenearsonate-acetyl bovine serum albumin.

A strong delayed-type footpad reaction was established in mice using azobenzenearsonate-acetyl bovine serum albumin (ABA-AcBSA) as an antigen. Male ddY/S mice were sensitized by subcutaneous injection of 100 microliter of Freund's complete adjuvant - saline (1:1) emulsion containing 100 microgram of the antigen and challenged by subcutaneous injection of 2.5 microliter of Freund's incomplete adjuvant - saline (1:1) emulsion with 2.5 microgram antigen in the footpad on the 10th day after the sensitization. The analysis of the system which fulfilled criteria for delayed-type hypersensitivity with regard to kinetics, passive transfer and histology of the footpad reaction, and the effect of dexamethasone, indomethacin and quinacrine on the footpad reaction were described.

Animals↗
PubMed1978

Chronic intestinal bleeding due to arteriovenous malformation of the colon.

A case of chronic intestinal bleeding due to arteriovenous malformation of the ascending colon was reported. Vascular abnormalities of the bowel represent the most common cause of gastrointestinal bleeding, the source of which could not be dislosed by conventional diagnostic procedures; selective abdominal angiography is the only most reliable technique in such cases. An acquaintance with these conditions may be of importance in the search for the cause of intestinal hemorrhages of otherwise obscure origin, and more attention should be paid to the clinicopathologic features of this entity.

Arteriovenous Malformations↗
PubMed1978

Enzyme immunoassay of human chorionic gonadotropin employing beta-galactosidase as label.

An enzyme-linked immunoassay was applied to the determination of hCG, a glycoprotein hormone usually assayed by RIA. For this purpose, an enzyme hormone conjugate was prepared by reacting hCG with beta-D-galactosidase (beta-Gal.) of E. coli in the presence of N-(m-maleimidobenzoyloxy) succinimide (MBS) as coupling reagent. The conjugate, after purification by affinity and gel chromatographies, was shown to exhibit sufficient enzyme activity and immunoactivity. The immunoassay of hCG was performed by the double antibody method and, using this assay, 0.4-250 mIU/ml hCG were detectable. This was about 10 times as sensitive as the RIA. Difficulty was experienced when this method was utilized for the determination of hCG in plasma samples from patients. Since the presence of the plasma may have affected this assay method, the following improvements were made: 1) the same volume of hormone-free plasma was added to the standard solutions of hCG, and 2) the volume of plasma sample was 10 microliter. The performance and validity of this assay were comparable to the RIA using [125I]hCG as tracer. The dose-response curves of both assay have the same slope and there was no significant difference between the values (correlation coefficient, Y = 0.96X + 1.53).

Acylation↗