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Biomedical subjects

M Iqbal

Publications and source records attributed to M Iqbal.

154 records · Page 9Linked to original sources
PubMed1973

A comparative study of aldolase from human muscle and liver.

Aldolase was purified from human skeletal muscle and human liver by techniques capable of processing large quantities (10-20kg) of tissue. The methods used also proved convenient for isolating aldolase on a large scale from other mammalian and avian sources. Aldolase from both human liver and muscle was crystallized; each gave two crystalline forms, depending on the conditions of crystallization. X-ray studies on the muscle aldolase crystals suggest a close structural similarity between human and rabbit muscle aldolase. Aldolases from human muscle and liver have similar pH optima and pH stability but their stability to heat treatment differs. The effect of heat on the enzymes may therefore provide an easy means of distinguishing them. The kinetic constants K(m) and k(cat.) for these aldolases are similar to other mammalian aldolases. Amino acid analyses and tryptic peptide ;mapping' show that the primary structures of the two aldolases differ greatly.

Amino Acids↗
PubMedDate not supplied

Novel copper superoxide dismutase mimics and damage mediated by O2.-.

Metabolites of oxygen such as superoxide anions (O2.-), hydrogen peroxide (H2O2), and hydroxyl radicals (OH.) are potentially damaging to biological systems. Univalent reduction of oxygen produces O2.-, which may be converted to H2O2 and OH(.). The biological damage mediated by O2.- can be attenuated by a cytosolic copper- and zinc-containing enzyme known as superoxide dismutase (SOD). Certain transition metal complexes having properties similar to SOD may be useful in suppressing such damage. However, known complexes have either been ineffective in vivo or may have toxic side effects. We prepared mixed-ligand copper complexes of polyamine using biomolecules such as pyridine or imidazoles as secondary ligands. The choice of polyamines and biomolecules was made with the aim of producing products with low toxicity. Our studies suggest that these copper complexes act as mimics of SOD in a variety of O2.(-)-generating systems and may be effective SOD mimics for their usage to abrogate such an injury in biological systems. This manuscript provides a brief state-of-the-art review on SOD mimics including our own studies.

Amino Acid Sequence↗