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Biomedical subjects

M Inukai

Publications and source records attributed to M Inukai.

46 records · Page 3Linked to original sources
PubMed1982

Temperature-sensitive processing of outer membrane lipoprotein in an Escherichia coli mutant.

A mutant of Escherichia coli that accumulated prolipoprotein, a secretory precursor of the outer membrane lipoprotein, was isolated. The prolipoprotein accumulated in this mutant was modified by glyceride, but the in vitro cleavage of the signal peptide of the accumulated prolipoprotein was found to be temperature sensitive. The mutation appears to be located outside the gene for the lipoprotein, thus suggesting that the gene for the signal peptidase for the prolipoprotein was mutated.

Bacterial Outer Membrane Proteins↗
PubMed1981

Preferential selection of deletion mutations of the outer membrane lipoprotein gene of Escherichia coli by globomycin.

Globomycin is an antibiotic which inhibits the processing of the prolipoprotein. Eighty globomycin-resistant mutants were independently isolated from Escherichia coli K-12 which had a deletion mutation in chromosomal lipoprotein gene (lpp), but contained a plasmid carrying the wild-type lpp gene. Twenty-six of the mutants did not have the lipoprotein in the membrane fractions. From the analysis of the plasmids of these mutants, all of the lipoprotein-deficient mutations were found to be due to deletion mutations around the lpp gene.

Anti-Bacterial Agents↗
PubMed1980

Malioxamycin, a new antibiotic with spheroplast-forming activity. I. Producing organism, fermentation, isolation and characterization.

Strain No. 15748, identifies as Streptomyces lydicus, produces a new antibiotic malioxamycin. Malioxamycin is a water-soluble, amphoteric antibiotic with low molecular weight, having a valine and a malic acid moieties in its structure. It gave a positive ninhydrin and ferric chloride reactions. Malioxamycin inhibited the growth of some Gram-negative bacteria by acting on peptidoglycan synthesis in the cell wall and produced spheroplasts from those sensitive bacteria.

Anti-Bacterial Agents↗
PubMed1980

Malioxamycin, a new antibiotic with spheroplast-forming activity. II. Structural elucidation and total synthesis.

Malioxamycin (1) is a new antibiotic produced by Streptomyces lydicus No. 15748. The structure of malioxamycin has been determined by nmr and mass spectra to possess a hydroxamic acid bond between L-valine and D-malic acid. This structure was confirmed by total synthesis of the antibiotic with (R)-aminooxysuccinic acid and the active ester of L-valine.

Anti-Bacterial Agents↗
PubMed1978

Globomycin, a new peptide antibiotic with spheroplast-forming activity. I. Taxonomy of producing organisms and fermentation.

A new peptide antibiotic, globomycin, was found to be produced by four different strains of the actinomycetes. They were identified as Streptomyces halstedii No. 13912, Streptoverticillium cinnamoneum No. 15037, Streptomyces neohygroscopicus subsp. globomyceticus No. 15631 and Streptomyces hagronensis No. 17834, respectively. Fermentation of globomycin was conducted by conventional submerged culture for antibiotic production, in which 10 microgram/ml of globomycin was produced by cultivation of S. halstedii No. 13912 for 96 hours at 27 degrees C. Globomycin was named after its activity to form global-shape spheroplasts when Escherichia coli was incubated in the presence of this antibiotic.

Anti-Bacterial Agents↗
PubMed1978

Globomycin, a new peptide antibiotic with spheroplast-forming activity. II. Isolation and physico-chemical and biological characterization.

The peptide antibiotic globomycin was extracted from the culture filtrate of Streptomyces halstedii No. 13912, purified on silica-gel columns and crystallized from acetonitrile to give colorless needles. It is a neutral substance with m.p. of 115 degrees C and a molecular formula of C32H57N5O9. On amino-acid analysis, it gave serine, threonine, glycine and an unidentified amino acid. It is soluble in methanol, ethyl acetate and chloroform but sparingly soluble in water. The antimicrobial spectrum of globomycin revealed its specific activity against Gram-negative bacteria. Formation of spheroplasts was observed when Escherichia coli was grown in the presence of globomycin, indicating inhibition of the bacterial cell wall synthesis as its mode of action.

Anti-Bacterial Agents↗
PubMed1978

Globomycin, a new peptide antibiotic with spheroplast-forming activity. III. Structural determination of globomycin.

The structure of globomycin has been determined by mass, PMR and CMR spectra and by chemical degradation. Globomycin is a new cyclic peptide antibiotic composed of L-serine, L-allo-threonine, glycine, N-methylleucine, L-allo-isoleucine and 3-hydroxy-2-methylnonaic acid. Among these components, L-allo-threonine, L-allo-isoleucine and 3-hydroxy-2-methylnonaic acid are novel components from natural products. A newly developed mass analysis has been introduced for determining the diastereoisomers of allo-threonine.

Amino Acids↗