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Biomedical subjects

M Imamura

Publications and source records attributed to M Imamura.

At least 775 records · Page 43Linked to original sources
PubMed1977

Murine Type C viral envelope glycoprotein gp69/71 and lupus-like glomerulonephritis of New Zealand mice. An immunoperoxidase study.

The location of murine Type C viral envelope glycoprotein antigen in the glomerulonephritic kidneys of NZB and NZB/NZW F1 hybrid mice was analyzed by the indirect immunoperoxidase technique at light and electron microscopic levels. Immune complex deposits of the glycoprotein antigen were present in the glomeruli in mesangial and subepithelial sites. In addition to the glomerular depositions, viral envelope antigen was also present at the brush border of proximal tubular epithelial cells and in lymphoid cells infiltrating the kidneys of these mice.

Animals↗
PubMed1976

Inhibition of syngeneic tumor growth in rats immunized with allogeneic skin grafts.

The growth of a transplantable tumor (KMT-17) in syngeneic Wistar King Aptekman/Mk (WKA) rats was inhibited by preimmunization with allogeneic normal cells from Donryu strain rats. The phenomenon is referred to as allogeneic cell immunity. A slight inhibition was observed in rats immunized with normal liver, spleen, kidney, embryonal cells, whole blood and white blood cells from allogeneic Donryu rats. A strong inhibition was observed in animals which had rejected allogeneic skin grafts, particularly from the Donryu and Kyoto rats. The inhibition was comparatively weak after immunization with skin grafts from the Long Evans, ACI, Buffalo, Ficsher, Sprague Dawley or Tokyo allogeneic rat strains. This immunizing effect was dependent on the viability of the grafts and was easily abrogated by low-dose irradiation. The mechanism of allogeneic cell immunity is considered to be a non-specific stimulation of the immunity against antigenic tumors in the syngeneic host

Animals↗
PubMed1976

The occurrence and frequency of type C virus-like particles in placentas from patients with systemic lupus erythematosus and from normal subjects.

Type C RNA virus-like particles were found by electron microscopy in term placentas from 3 patients with systemic lupus erythematosus (SLE), 1 with probable SLE, and 2 normal patients. The virus-like particles were mainly of a budding or immature type located at or near the cell membrane of syncytiotrophoblasts in chorionic villi. Type C virus-like particles were not observed in the term placenta from a patient with chronic discoid LE, nor in early gestation specimens from 1 normal patient and 4 patients with SLE. The frequency of the Type C particles varied greatly: They were readily found in the patient with probable SLE, and only here were groups of budding particles observed. Type C particles were less numerous in one normal placenta and rare in the other positive placentas, both SLE and normal. Heretofore undescribed crystalline inclusions were found in the cytoplasm of chorionic villous endothelial cells from 3 patients with SLE and 1 with discoid LE. Tubuloreticular structures were observed in the maternal endometrium of 1 patient with SLE.

Chorionic Villi↗
PubMed1975

Hemodynamic changes in the liver of the rabbit after hepatic dearterialization.

Quantitative determination of the development of collateral arteries and change in portal flow after an 80 per cent dearterialization of the liver of the rabbit was carried out with radioactive microsphere technique. The livers operated upon were classified macroscopically into two type groups. In the recovery type, the dearterialized lobes reverted to the preoperative state. In the necrotic type, necrosis in the dearterialized lobe progressed, and the nonarterialized lobe was compensatively hypertrophied. The rate of rearterialization into the dearterialized lobe was different according to the type. In the recovery type, collateral arteries developed rapidly until the fourteenth postoperative day, when the arterial flow reached 55 per cent and, thereafter, increased gradually. In the necrotic type, rearterialization progressed more rapidly, and arterial flow exceeded the preoperative value in the third postoperative week and, thereafter, decreased. In the necrotic type, portal flow in the dearterialized lobe had been significantly impeded within 12 hours after the operation. The state of portal flow in the dearterialized lobe is, we believe, a more important factor for the prognosis after the hepatic dearterialization than the rate of rearterialization or oxygen concentration of portal blood. High activity of serum glutamic oxalacetic transaminase and serum glutamic pyruvic transaminase of more than 90 units per milliliter on the seventh postoperative day can be an indicator for the impeded portal flow in the dearterialized lobe.

Alanine Transaminase↗
PubMed1975

Liver metabolism and glucogenesis in trauma and sepsis.

The relationship of glucogenesis and other energy-requiring functions of the liver to the proteolysis which is characteristic of trauma and sepsis was studied in conscious pigs following laporotomy and after the induction of intraperitoneal sepsis. By means of appropriately placed thermal dilution catheters, portal and hepatic arterial blood flows, hepatic oxygen consumption, glucogenesis, and uptake of the fuel, substrates were measured. No animal was in shock. Despite significant increases of lactate and aminoacids delivered to the liver, the blood concentrations were maintained in the normal range. The rate of glucogenesis was proportional (r equals 0.71) to the sum of the glucogenic precursors (lactate, pyruvate, glycerol, and alanine) taken up by the liver. Higher rates of glucose production were accompanied by elevated blood insulin values. Hepatic oxygen consumption and the uptake of free fatty acids also were related directly to the glucogenic rate, the correlation coefficients being 0.69 and 0.74, respectively. In the absence of shock, the liver function and hepatic energy production remained normal in post-traumatic and septic states. Under the conditions insulin-resistant muscle in the presence of reduced free fatty acid availability mobilize protein to satisfy local energy requirements. Skeletal muscle can oxidize only branch chain aminoacids; other aminoacids, including alanine, are transported to the liver for glucogenesis or other purposed. This concept accounted for failure of glucose infusion to eliminate post-traumatic and septic proteolysis, since alanine is cleared only from blood by conversion in the liver to glucose. Thus it is concluded that in sepsis the release of glucogenic substrates because of altered metabolism in peripheral tissues determines the rate of hepatic glucogenesis. This relationship constitutes an important metabolic homeostatic mechanism.

Alanine↗
PubMed1975

Hepatic blood flow and oxygen consumption in starvation, sepsis and septic shock.

Total hepatic and portal blood flow as well as hepatic and splanchnic oxygen consumption was measured in pigs in the normal fasted state and in the septic fasted state induced experimentally by cecal ligation. Pigs in the septic state were divided into two groupds, a septic high output state and a septic low output state, according to whether or not a pig showed a higher or lower cardiac output in the septic state than in the normal fasted state. In the septic high output state, the average cardiac output was 155 per cent of that during fasting; hepatic arterial flow increased 96 per cent, while portal flow decreased 19 per cent. Total hepatic blood flow increased slightly, 14 per cent. The ratio of total hepatic blood flow to cardiac output decreased from 42 per cent in the normal fasted state to 30 per cent. The total hepatic oxygen consumption increased 26 per cent because of significantly elevated oxygen transport by hepatic arterial flow. In the low output state, hepatic arterial flow significantly dropped, 74 per cent, while portal flow decreased slightly, 23 per cent, and total hepatic blood flow decreased 38 per cent. The decrease of cardiac output was less, 18 per cent. The ratio of total hepatic blood flow to cardiac output was 31 per cent. Total hepatic oxygen consumption decreased remarkably, 29.1 per cent, due chiefly to the decrease of hepatic arterial flow, and splanchnic oxygen consumption also decreased significantly, 22 per cent. Dissociation of hepatic arterial response to sepsis from the other gastrointestianl vessels caused a great difference in the hepatic oxygen consumption between the septic high output and the septic low output state, contributing in part to the difference in mortality between these two states.

Animals↗
PubMed1975

Electron microscopic study of distinctive structures in peripheral blood lymphocytes obtained from twins with systemic lupus erythematosus.

Peripheral blood lymphoid cells obtained from nine twin pairs (six monozygotic and three dizygotic) in which one or both twins had systemic lupus erythematosus (SLE) were examined by electron microscopy for the occurrence of two distinctive intracytoplasmic structures-tubuloreticular structures (TRS) and tubular crystalloids (TC). TRS were found in 0.8 to 14.8% of lymphoid-cell cross sections in 9 of 11 twins with SLE and 2 clinically well but serologically abnormal twins. Lymphoid cells of twins both clinically and serologically normal did not exhibit TRS, although their monozygotic or dizygotic SLE-positive counterparts possessed these structures. Thus, the expression of TRS was more consistent with an acquired than inborn trait and appeared to correlate with disease and serologic manifestations of SLE. TC were found in 1.7 to 7.9% of lymphoid-cell cross sections in every twin examined. No correlation was recognized between clinical or laboratory data and the frequency of TC-bearing cells. The significance and the ultrastructural development of TC in the peripheral blood lymphoid cells are briefly discussed.

Diseases in Twins↗