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Biomedical subjects

M Ichijo

Publications and source records attributed to M Ichijo.

At least 145 records · Page 8Linked to original sources
PubMed1985

[Fundamental and clinical studies of aztreonam in the field of obstetrics and gynecology].

Aztreonam (AZT, E-0734), a new beta-lactam antibiotic, was fundamentally and clinically studied. The following results were obtained. The serum and internal genital tissue levels for AZT after 1 g intravenous injection had been kept at more than about 20 micrograms/ml and 3.0 micrograms/g, respectively, during 1 hour. AZT was administered at 1-2 g of daily dose by intravenous injection or intravenous drip infusion to 5 patients with obstetric and gynecological infections, comprising 1 of pyometra, parametritis, Bartholin's abscess, puerperal endometritis and diffuse peritonitis. Clinical efficacy was; excellent in 1 puerperal endometritis case, good in 2 cases and poor in 2 cases. Neither side effect nor abnormal laboratory finding was observed.

Adult↗
PubMed1985

[Nephritogenic glycopeptide (nephritogenoside): its existence in the urine of toxemia patients].

Previously we reported that we succeeded in isolating from human full term placenta, glycopeptide which was immunologically the same as nephritogenic glycopeptide (Nephritogenoside) prepared from human renal GBM (glomerular basement membrane). This time to clarify the correlation between the pathology of toxemia and Nephritogenoside, we examined the existence of Nephritogenoside in the urine of toxemia patients. To purify Nephritogenoside from the urine powder of toxemia patients, we use the same procedures (enzyme digestion, Con A affinity column chromatography e.t.c.) as used in purifying Nephritogenoside from human full term placental TrBM (trophoblast basement membrane). In the urine of toxemia patients we clarified the existence of glycopeptide, which had specific affinity with Con A and was immunologically the same as Nephritogenoside. The existence of Nephritogenoside in the urine of toxemia patients further strengthened the possibility of a relationship between Nephritogenoside and the pathology of toxemia.

Electrophoresis↗
PubMed1985

[Study on the mechanism of placental transport of L-lysine (using human placental microvillous (brush border) membrane vesicles)].

The uptake of L-lysine in human placental microvilli vesicles prepared from human term placenta was studied using the rapid filtration technique. The uptake of L-lysine into the vesicles was osmotically sensitive. This finding indicates that the uptake of L-lysine by placental microvilli vesicles represents transport into the membrane vesicles. The uptake of L-lysine into microvilli vesicles is sodium independent. The initial rate of uptake is markedly increased when the intravesicular space is rendered electrically more negative by membrane diffusion potential induced by the use of highly permeant anions or by K+ diffusion potentials via valinomycin. These results indicate that the sodium independent uptake of L-lysine into the microvilli membrane vesicles is dependent on the electrical potential difference of the membranes. A kinetic analysis of the uptake demonstrated that two transport systems for vesicular entry of L-lysine existed with a Km1 of 0.13 mM, Vmax1 of 590 pmol/mg protein/20 sec, Km2 of 0.91 mM, Vmax2 of 2010 pmol/mg protein/20 sec. The uptake of L-lysine in microvilli vesicles was inhibited by dibasic amino acid (L-arginine, L-ornithine, L-glutamine), but not by other classes of amino acid. These results indicate the existence of a dibasic amino acid specific transport system in placental microvilli membrane.

Arginine↗
PubMed1985

[Clinical trial and basic study on the tissue transfer of cefminox].

Cefminox (CMNX, MT-141) was studied both fundamentally and clinically with following results: In the treatment of 3 cases of Bartholin's abscess, 2 cases of pyometra, and 1 case each of bartholinitis, inflammation of the pelvic dead space, retroperitoneal abscess and pelvic peritonitis, CMNX was administered at a dosage of 1 g. The global clinical results were rated as good in 9 cases. From these findings it is considered that CMNX is promising as an antibiotic with extremely high efficacy for infections of the field of obstetrics and gynecology. Furthermore, since in none of our cases side effects or laboratory abnormalities were observed, CMNX is considered to be a drug with high efficacy and safety. In 5 cases received 1 g of CMNX intravenously, concentrations of the drug in the serum and tissues of internal genital organs were determined. CMNX was maintained at concentrations higher than 20 micrograms/ml for the serum and 10 micrograms/g for each tissue studied. In the pelvic dead space exudate 10 to 20 micrograms/ml of the drug was still detected even at 8 hours after the administration. These results obtained by our fundamental study support the efficacy of CMNX demonstrated in the clinical part of our study.

Adult↗
PubMed1985

[Studies on L-glutamate transport mechanism in human placental trophoblast microvilli membrane vesicles].

The uptake of L-glutamate in brush border (microvilli) vesicles prepared from human term placenta was studied using the rapid filtration technique. The uptake of L-glutamate into the vesicles occurred osmotically, and preincubation with L-glutamate increased the uptake of amino acid. These findings indicate that the uptake of L-glutamate by placental trophoblast brush border membranes represents the transport into membrane vesicles. A Na+ electrochemical gradient (extravesicular greater than intravesicular) stimulated the initial rate of L-glutamate uptake about three times. The initial rate of transport exhibited saturation kinetics with respect to the L-glutamate concentration; an apparent Km of 0.15 mM and V max of 70 pmol/mg protein in 20 seconds were calculated. The uptake of L-glutamate into the vesicles was competitively inhibited by L-glutamate and L-cysteate (acidic amino acid). These results indicate that a Na-dependent acidic amino acid specific transport system exists in the placental trophoblast microvilli membrane. These results indicate that the transport of L-glutamate across the placental microvilli membrane is sodium-dependent and carrier mediated.

Aspartic Acid↗
PubMed1985

[Tissue polypeptide antigen in gynecological cancer].

Serum tissue polypeptide antigen (TPA), CEA and IAP were measured simultaneously in cervical cancer, corpus cancer and ovarian cancer. TPA levels were increased (more than 110 U/1) in 39% (32/82) of the cervical cancer and in 46% (6/12) of the corpus cancer patients, respectively. In ovarian cancer, TPA levels were elevated (146 U/1 or higher) in 64% (13/22). The positive rate of CEA was somewhat lower than that of TPA and LAP levels were as high as TPA. Moreover, TPA levels were correlated in clinical course. In immunohistochemical examination, TPA was present in cancer cells and absent in normal tissue.

Adenocarcinoma↗
PubMed1985

[Studies on the mechanism of placental transport of L-glutamate (the effect of K+ in microvillous vesicles on L-glutamate uptake)].

The effect of potassium ion and membrane potential on the uptake of L-glutamate in microvilli (brush border) vesicles prepared from human term placenta was studied using a rapid filtration technique. The uptake of L-glutamate into microvilli vesicles is Na+ electrochemical gradient dependent and pre-equilibration of the vesicles with K+ stimulates L-glutamic acid uptake. Imposition of a K+ gradient (K+ in greater than K+ out) enhances Na+-dependent L-glutamate uptake. Changes in membrane potential due to the imposition of anion replacement markedly affect Na+ dependent L-glutamate uptake only in the presence of K+. However, this effect is not significant when changes in membrane potential incur following the imposition of valinomycin induced by K+ diffusion potential. The data indicate that Na+-dependent L-glutamate transport can be additionally energized by a K+ gradient. Furthermore K+ renders Na+-dependent L-glutamate transport sensitive to changes in the transmembrane potential difference.

Cell Membrane Permeability↗
PubMed1985

[Fundamental and clinical studies of cefpiramide in the field of obstetrics and gynecology].

Cefpiramide (CPM, SM-1652), a new cephem antibiotic, was fundamentally and clinically studied. The following results were obtained. Serum and internal genital tissue levels of CPM were measured following intravenous drip infusion of 1 g. High serum levels of 30 micrograms/ml and tissue levels of more than 4 micrograms/g were at least maintained for 8 hours. Favourable transfer of CPM into the pelvic dead space exudate was observed. The exudate level was 7.25 micrograms/ml on average even at 8 hours after intravenous drip infusion. A total of 6 cases comprising 4 with Bartholin's cyst, 1 with pelvic peritonitis and 1 with lymphocyst was treated with CPM at a dose of 0.5-2 g twice daily by intravenous injection or intravenous drip infusion. The clinical response was excellent in 1 case and good in 5 cases. Side effects and abnormal laboratory findings due to the drug were not noted.

Adult↗
PubMed1985

[Study on the mechanism of placental transport of phosphate (using human placental microvillous (brush border) membrane vesicles)].

Using the rapid filtration technique, the uptake of phosphate into microvillous (brush border) membrane vesicles isolated from human placental trophoblast was investigated. The microvillous membrane vesicles exhibit the uptake of phosphate into an osmotically reactive intravesicular space and preincubation with the phosphate increased the uptake of phosphate into the vesicles. These findings indicate that the uptake of phosphate by placental trophoblast microvillous membranes represents transport into membrane vesicles. In the presence of sodium electrochemical gradient (extravesicular greater than intravesicular) the uptake of phosphate by vesicles shows an overshoot phenomenon. Sodium-dependent phosphate uptake is about two times higher at pH 6.0 as in the uptake observed at pH 8.0. The initial rate of sodium-dependent phosphate transport exhibited saturation kinetics with respect to phosphate concentration: An apparent Km of 0.28 mM and Vmax of 330 pmol/mg protein in 20 seconds were calculated. These results indicate that the transport of phosphate across the microvillous membranes is carrier mediated. Experiments with different anions (SCN-, Cl-, gluconate-) and ionophore (valinomycin) showed that at pH 7.4 phosphate transport in the presence of a Na+ gradient is almost independent of electrical potential across the vesicle membranes. It was concluded that placental trophoblast microvillous membranes contain an electroneutral Na+/phosphate co-transport system.

Anions↗
PubMed1984

[Placental transport of taurine in brush border microvilli].

Placental transport of taurine was studied in isolated brush border microvillous plasma membrane vesicles by a rapid filtration technique. Brush border microvillous plasma membrane vesicles were prepared from syncytio trophoblast of human term placenta by a method of differential centrifugation and calcium precipitation. The specific activities of alkaline phosphatase, 5' nucleotidase and gamma-GTP in the membrane preparation were enriched to 13-14 times, 12-13 times, and 5-6 times respectively, as high as those in the homogenate. The membrane vesicles exhibit uptake of 3H-labeled taurine into an osmotically reactive intravesicular space. Taurine uptake by vesicles was stimulated specifically by an inward sodium gradient, and replacement of NaCl in the transport medium by KCl, LiCl, and choline chloride had no effect on the transport activity of the vesicles. Taurine transport is inhibited competitively by the presence of beta alanine and GABA. The initial rate of transport followed saturation kinetics with respect to the taurine concentration: An apparent Km of 0.22mM and Vmax of 67 pmol/mg protein were calculated in 20 seconds. These results indicate that transport of taurine across the placental brush border membrane is sodium dependent and carrier mediated.

Alkaline Phosphatase↗
PubMed1984

[Clinical studies on sulbactam/cefoperazone in the field of obstetrics and gynecology].

Sulbactam/cefoperazone (SBT/CPZ), a new developed antibiotic, was clinically studied. The following results were obtained. Total of 5 cases comprising 1 with endometritis, 3 with Bartholin's abscess, 1 with pyometra (due to corpus cancer) were treated with SBT/CPZ at a dose of 1 g twice daily for 4-5 days by intravenous injection or intravenous drip infusion. The clinical response was good in all cases. A case with pyometra did not respond to the therapy in bacteriologically. Side effects and abnormal laboratory findings due to the drug were not noted.

Adult↗
PubMed1984

[Fundamental and clinical study on ceftriaxone in the field of obstetrics and gynecology].

It is reported that ceftriaxone (Ro 13-9904, CTRX) has a half-life time of 7 to 8 hours. In the present study, the serum level 18 hours after intravenous injection with 1 g CTRX was as high as 9.3 micrograms/ml while obviously a higher tissue concentration was maintained compared with other drugs. These facts suggest that CTRX is effective against infections and that the dosage and frequency of administration could be reduced. The global evaluation revealed that CTRX was clinically effective in all the 4 cases with infections. As the adverse reaction, light leukopenia was observed only in 1 case out of the present 4 cases and 20 others administered with CTRX.

Aged↗
PubMed1984

[Fundamental and clinical studies of ceftazidime in the field of obstetrics and gynecology].

Ceftazidime ( CAZ ), a new cephalosporin antibiotic, was fundamentally and clinically studied. The following results were obtained. Serum and internal genital tissue levels of CAZ were measured following intravenous drip infusion of 1 g for 30 minutes. Serum levels of more than 10 micrograms/ml and tissue levels of more than about 7 micrograms/ml were maintained after 2 hours to 2 hours and 30 minutes, respectively. Favourable transfer of CAZ into the pelvic dead space exudate was observed. The exudate level attained its peak of 31.54 micrograms/ml on average at 2 hours and was 16.8 micrograms/ml on average even at 8 hours after intravenous drip infusion. A total of 6 cases comprising 1 of adnexitis, 2 of pyometra, 1 of endometritis and 2 of parametritis was treated with CAZ at a dose of 0.5 approximately 2.0 g twice daily by intravenous injection or intravenous drip infusion. The clinical response was excellent in 1 case, good in 4 cases and poor in 1 case. Abnormal laboratory findings and side effects due to the drug were not noted.

Adult↗