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Biomedical subjects

M I Skikne

Publications and source records attributed to M I Skikne.

10 recordsLinked to original sources
PubMed1982

Chordoid sarcoma of the hand. A case report.

Chordoid sarcoma is a rare, distinctive neoplasm that usually occurs in the soft tissue of the extremities. Despite its striking histological resemblance to chordoma at light microscopic level, both clinical and ultrastructural differences exist and chordoid sarcoma may in fact be related to tendosynovial sarcoma. Previous reports of extraskeletal myxoid chondrosarcoma, however, show that this tumour bears a striking light microscopic and ultrastructural similarity to chordoid sarcoma, which suggests that the latter may be a variant of extraskeletal myxoid chondrosarcoma. In this report a further case of chordoid sarcoma is presented; clinical, radiological, histological and ultramicroscopic features support a tendosynovial origin.

Adult↗
PubMed1980

Submicroscopic fibres in industrial atmospheres.

Evidence obtained from a large number of samples of airborne dust from a number of different asbestos-using industries suggests that it would be possible to use gravimetric sampling to estimate the respirable fibre concentration of the atmosphere around specific operations. The precision of this method of estimating airborne fibre concentration is shown to be approximately the same as the variation between different counters using existing counting techniques. Tables are included showing the fibre content of industrial dusts in millions of fibres/mg of dust and the factors (with 95% confidence limits) by which light microscope counts should be multiplied to give the true counts of fibres longer than 5 micrometers.

Air Pollutants↗
PubMed1979

Biochemical studies of the matrix of craniovertebral chordoma and a metastasis.

Glycosaminoglycans of human chordoma and a metastasis were studied. The glycosaminoglycans were derived from the extracellular matrix and consisted of chondroitin 4- and 6-sulphate, keratan sulphate and hyaluronate. The ratio of chondroitin sulphate to keratan sulphate was much lower in the metastasis than in the primary. Proteoglycan extracted with 4M guanidinium chloride and purified by associative density-gradient centrifugation was assessed on Sepharose 2B before and after reduction and alkylation. The result of this experiment suggested that only a small proportion of the proteoglycans were aggregated.

Chordoma↗
PubMed1978

Ultrastructure of the lung in the rat following exposure to crocidolite asbestos and quartz.

Lung tissue from rats that had inhaled U.I.C.C. crocidolite asbestos or quartz particles showed thickening of the interstitial tissue and the presence of collagen fibres. Aggregates of macrophages in the alveolar spaces were a dominant feature of all the sections examined from asbestos exposed rats. According to the ultrastructural studies described here, all the macrophages were mature cells, indicating that the ingested crocidolite asbestos was non-toxic. Lung sections of rats exposed to quartz particles were significantly different. Single cells were found in the alveolar spaces and many macrophages showed a characteristic pattern of vacuole formation. Other cells contained intracellular membranous lamellated bodies, similar to those found in Type II pneumocytes. Cells containing lamellated bodies were also found in the interstitial tissue. These findings suggest that the two mineral dusts have quite different biological effects on the macrophage and that the development of pulmonary fibrosis may, to some extent, be caused by a different mechanism in each instance.

Animals↗
PubMed1976

The prenatal diagnosis of Tay-Sachs disease.

Tay-Sachs disease was diagnosed prenatally on the basis of enzyme assays and the electrophoretic pattern of extracts made from cultured amniotic fluid cells. Electrophoresis revealed the presence of hexosaminidase B but no hexosaminidase A, the iso-enzyme which is deficient in Tay-Sachs disease. Estimations of the percentage of hexosaminidase A present in cultured amniotic cell extracts by means of thermal fractionation revealed the presence of only small amounts of this isozyme. Results obtained on amniotic fluid alone were found to be unreliable and were disregarded when making the diagnosis. Following termination of the pregnancy, the diagnosis was confirmed by electrophoresis and the thermal inactivation method as well as by electron microscopy. A slight lower temperature for the thermal inactivation has been found to give better discrimination than the temperature used in previously described techniques.

Amniotic Fluid↗