Biomedical subjects

M I Romano

Publications and source records attributed to M I Romano.

39 records · Page 3Linked to original sources

PubMed1983

The influence of estrogen administration on the subcellular receptors distribution and its biological effect in the rat anterior pituitary gland.

We studied the effects of estrogen administration in the anterior pituitary gland (APG) of male rats. The subcellular distribution of estradiol receptors, and some biological responses, such as prolactin secretion and progestine receptors were examined. In the presence of circulating estrogens, the binding of [3H]-estradiol decreased in the cytosol and increased in nuclei. The total binding augmented significantly (P less than 0.05). These changes were dose and time dependent. So, in long chronic treatment, the changes in subcellular receptor distribution were greater, in accordance with higher serum estrogen level. Serum prolactin concentration and progestin receptor in the APG increased continuously as a function of time of the estrogen treatment. They were related to the presence of nuclear estradiol receptors, which remained elevated throughout the entire experimental period.

Animals↗

PubMedDate not supplied

A western blot characterization of Mycobacterium bovis antigens recognized by cattle sera.

The immune response to Mycobacterium bovis in cattle was assessed by Western blot. The antibody recognition pattern to M. bovis whole cell extracts and culture supernatant antigens was studied by using sera from M. bovis-infected (n = 62) and healthy (n = 38) cattle. Although the recognition patterns were highly variable, some proteins were regularly detected, mainly those with molecular masses of 17, 23, 28, 42, 66, 71 and 80 kDa in cellular extracts, and with molecular masses of 23 and 33 kDa in supernatants. Whole cell extract antigens were more frequently recognized than culture supernatant antigens. Healthy controls produced only a weak antibody response. The antibody response was variable, depending on tuberculosis stage. In early stages very few antibodies were detected. A response against the 66-kDa stress protein was mounted in intermediate tuberculosis and remained stable in more advanced disease. In late diseases, the preferentially recognized antigens were a 28-kDa cellular protein and supernatant antigens. The 28-kDa protein was studied in some detail. As determined by using monoclonal antibodies, the 28-kDa protein is different from superoxide dismutase. This protein aggregated in stored cell extracts and was not totally transferred to nitrocellulose. The principal conclusions of this work are: (i) whole cell extract proteins are more frequently recognized than the secreted proteins and (ii) a 28-kDa protein is a major antigen in late disease.

Animals↗

PubMedDate not supplied

[The genotype of the principal Mycobacterium bovis in Argentina is also that of the British Isles: did bovine tuberculosis come from Great Britain?].

Bovine tuberculosis is a highly prevalent animal disease in Argentina. In this work evidence was obtained showing that a major Mycobacterium bovis group in Argentina had been introduced with the bovine bulls imported from the United Kingdom at the end of the XIX century. This evidence came from two sources: historical, obtained by bibliographical references, and from laboratory results, using a molecular typing method called spoligotyping. These strains are also present in other countries that introduced cattle from the same origin.

Animal Husbandry↗