Biomedical subjects

M I Levi

Publications and source records attributed to M I Levi.

At least 73 records · Page 4Linked to original sources

PubMed1975

[Approximate assessment of the successfulness of bacteriological and serological methods of studying rodents in natural plague foci].

On the basis of the study of two mathematical models of the epizootics the authors suggest a criterion for the determination of the correlation of the value of the results of bacteriological and serological examination of rodents in the natural foci of plague. In accordance with the criteria obtained an evaluation was made of this correlation for Pallasiomys meridianus of the left and right banks of the Volga. It appeared that the calculated assessment of the value of the results of the two methods under study correlated satisfactorily with the data obtained in practice during the epizootologic examination of the Volgo-Urals natural focus of infection.

Animals↗

PubMed1975

[Utilization of a solid nutrient medium for the serological method of detecting the causative agent of typhoid in patients' feces].

The causative agent of typhoid fever can be revealed in feces of patients not only by the bacteriological, but also by the serological method, if the material is preliminarily grown on hard nutrient media; Ploskirev's medium proved to be the optimal. It is suggested that microbial cultures left on the ager in Petri dishes after the bacteriological study should be used for bacteriological analysis. In a number of cases it was possible to reveal the causative agent of typhoid fever in feces by the serological method, although no coproculture was isolated bacteriologically.

Antigens, Bacterial↗

PubMed1975

[Serological study of nontriturated fleas following preliminary cultivation of the plague microbe].

Under experimental conditions there was shown a possibility of serological study of nontriturated fleas for the presence of Past. pestis. Hottinger's broth with gentian-violet ((0.0003%) sodium sulfite (0.03%) and a surfactant--O (see article)-7 (3%) served as the growing medium. After the nontriturated fleas were grown for 48 hours at 37 degrees C the capsular antigen was detected with the aid of the passive hemagglutination and antibody neutralization tests. Detection of the capsular antigen in the growing medium pointed to the presence of Past. pestis in the fleas. To test the serological method of study the nontriturated fleas, the insects were collected in the colonies of Rhombomys opimus Licht. at the epizootic territory. The collected fleas were divided into portions (each portion contained not more than 20 fleas). Half of the portions of each collection was studied after trituration by the usual bacteriological method, and another--by serological method without trituration of the insects. In examining the parallel portions (a total of 2400 portions, about 32 000) it appeared that positive results were obtained by both methods with about the same frequency.

Animals↗

PubMed1975

[The possibility of predicting the degree of plasmacyte reaction in response to administration of soluble antigen to mice].

The work is devoted to the mathematical description of the accumulation of plasma cells in the spleen of CBA mice immunized intraperitoneally. A dependence between the dynamics of the soluble antigen concentration in the blood and the plasmocytic reaction in the spleen was confirmed under experimental conditions. Index A was suggested for detection of a change from the antigenic function to the accumulation of plasma cells. The mean values of index A were used to compare the calculated values of the plasmocytic reaction and the experimental data; differences between these values were insignificant. Analogously index A was used for predicting the accumulation of plasma cells in the animals to which a mixture of the two soluble antigens (a capsular antigen of the causative agent of plague and a complete antigen of the causative agent of tularemia) was administered. The calculated values of the plasmocytic reaction in this experiment satisfactorily correlated with the data obtained experimentally.

Animals↗

PubMed1974