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Biomedical subjects

M I Gubarev

Publications and source records attributed to M I Gubarev.

6 recordsLinked to original sources
PubMed1998

Localization to chromosome 11 of a gene encoding a human minor histocompatibility antigen.

The graft-versus-host disease (GVHD) seen in human leukocyte antigen (HLA)-matched sibling bone marrow transplants is by definition due to the "minor" histocompatibility antigens (mHAs) encoded outside the HLA region of human chromosome 6. Few of these antigens have been characterized in humans, and in general the locations of the encoding loci are unknown. Genetic experiments performed in mice have identified many mHAs, but only a few genes have been identified. Using T lymphocyte clones reactive with specific mHAs, combined with genetic linkage analysis, we identified two distinct loci in a single patient, each locus encoding an antigen presented to a T cell clone by HLA-B7. The technique used in this study should allow a rough enumeration of the number of mHAs in humans that are capable of eliciting T cell responses in vivo. Whether these T cell responses correlate with clinical GVHD is not yet clear.

Bone Marrow Transplantation↗
PubMed1996

Localization to chromosome 22 of a gene encoding a human minor histocompatibility antigen.

In human allogeneic bone marrow transplantation, graft-vs-host disease and graft rejection can occur even if the patient and donor are genotypically matched by inheritance for HLA. By definition, these allogeneic reactions are due to disparities in minor histocompatibility Ags (minor HAs). Minor HAs are presented to T lymphocytes as peptides bound to HLA molecules, and appear to be encoded by genes throughout the genome. We derived T lymphocyte clones from the PBL of a patient suffering from chronic graft-vs-host disease after bone marrow transplant from his HLA-identical sister. Clones reactive against minor HAs were selected on the basis of reactivity with pretransplant patient cells, and absence of reactivity with donor cells. One clone (MD2) was found to use HLA-B7 as a restricting element. A plasmid vector (pHEBo) containing cDNA encoding the HLA-B7 molecule was transfected into lymphoblastoid cell lines derived from two large families that previously had been saturation mapped for hundreds of polymorphic loci. When clone MD2 was tested against family K1362, it was found to be reactive with three of four grandparents, both parents, and eight of eleven offspring. The same clone was tested with family K1331, with two of three tested grandparents reactive, one of two parents, and nine of eleven offspring. Computer analysis showed that both family segregation patterns linked to an area on the long arm of chromosome 22, localizing the gene encoding this minor HA near the platelet-derived growth factor-beta and IL-2Rbeta genes.

Chromosome Mapping↗
PubMed1989

[The protective effect of preparations made from plant seedlings in experimental Pseudomonas aeruginosa infection].

The influence of preparations obtained from oat and wheat seedlings (immunostimulating factors IF-1 and IF-2, respectively) on the natural resistance of mice to P. aeruginosa infection was studied. IF-1 and IF-2 were introduced intraperitoneally in a single injection in doses of 100 micrograms and 1000 micrograms per mouse 2 and 7 days prior to the inoculation of P. aeruginosa strain 8 in doses of 1 and 10 LD50. The presence of substances capable of stimulating the immunobiological reserves of the body in actively growing plants (seedlings) was shown.

Adjuvants, Immunologic↗
PubMed1986

[Bone marrow suppressor cells of patients with viral and alcoholic liver cirrhoses].

A study was made of bone marrow suppressor cells potencies in 12 viral and alcoholic liver cirrhosis patients and 6 healthy persons using a method of the registration of bone marrow suppressor cells activity in the inhibition of xenogenic target cells proliferation. The activity of bone marrow suppressor cell in viral and alcoholic liver cirrhosis was significantly lowered as compared to that of the healthy donors. Besides, the spontaneous bone marrow cells proliferation level both in viral and alcoholic liver cirrhosis exceeded essentially that of the healthy donors. It might be due either to the reduced number or decreased function bone marrow suppressor cells in liver cirrhosis. These patients' peripheral blood lymphocytes under in vitro conditions as well as the healthy donors' lymphocytes did not produce any suppressive effect.

Adult↗
PubMed1985

[Normal human bone marrow suppressor cells and in liver cirrhosis].

Suppressor properties of bone marrow cells were studied in healthy donors and patients with hepatocirrhosis using the technique registrating the activity of bone marrow B-suppressors by the inhibition of xenogenic target cell proliferation. The activity of bone marrow suppressor cells in patients with various types of hepatocirrhosis was reduced as compared to healthy subjects. In addition, the in vitro spontaneous proliferation level of bone marrow cells in hepatocirrhosis was considerably higher than that of healthy donors. This fact can be possibly attributed to the decline in the number of bone marrow B-suppressors or inhibition of their functional activity in hepatocirrhosis. Peripheral blood lymphocytes of these patients, like the lymphocytes of healthy donors, showed practically no suppressive effect in vitro.

Animals↗