Biomedical subjects

M Hyodo

Publications and source records attributed to M Hyodo.

At least 55 records · Page 3Linked to original sources

PubMed1992

[The clinical effect of intravenous regional sympathectomy with guanethidine in the management of sympathetically maintained pain].

We treated 25 patients suffering from sympathetically maintained pain with intravenous regional sympathectomy with guanethidine. Of these patients, 18 had reflex sympathetic dystrophy; 5 had causalgia; and 2 had shoulder hand syndrome. The visual analog scale before IRS therapy was 6.1 +/- 0.3, and after therapy, it was 2.3 +/- 0.4. The mean rate of improvement was 54.2% in reflex sympathetic dystrophy and 81.0% in causalgia. The therapy was particularly effective in patients with shoulder hand syndrome. These results suggest that intravenous regional sympathectomy with guanethidine could be effective for sympathetically maintained pain.

Adult↗

PubMed1992

[Compression of the graft during the corrective surgery for scoliosis in a patient who has undergone a Rastelli's operation: a case study].

A 14 year-old male who had undergone Rastelli's operation for the tetralogy of Fallot had general anesthesia for the corrective spinal instrumentation and posterior fusion. The patient also suffered from the complication of Klippel-Feil syndrome. During the operation, right ventricular pressure gradually increased from 34 mmHg to 60 mmHg and systolic blood pressure decreased from 110 mg to 80 mmHg when the maneuver to correct spinal deformity was done. The hemodynamics returned to its former level after the maneuver was resumed. This change was presumably attributed to the fact that the external conduit was compressed by the sternum and vertebral bodies. In order to prevent this hazardous incidence, it is crucial to understand both the three-dimensional configuration of the thoracic structure and the influence of spinal correction on it. In the prone positioned patients with scoliosis, especially lordoscoliosis, compression or dysfunction of the artificial valve might occur and we have to be aware of the possibility of the compression during general anesthesia and after corrective surgery.

Adolescent↗

PubMed1992

[Mechanisms of hypoalgesia induced by intracisternal administration of PGD2 or PGE2].

Intracisternal administration of PGD2 (5 micrograms) and PGE2 (5 micrograms) induced hypoalgesia in conscious mice in acetic acid writhing test. The hypoalgesia caused by PGD2 was blocked by para-chlorophenylalanine, cyproheptadine or phenoxybenzamine. However, the suppression of writhing responses caused by PGE2 was not blocked at all by these drugs. These results indicate that the actions of PGD2 depend upon serotonin and norepinephrine systems.

Animals↗

PubMed1992

[Effect of botulinum A toxin injection in the management of hemifacial spasm].

The present study was carried out on the effect of botulinum A toxin, made in Japan, in the management of 30 hemifacial spasm patients. In almost all cases, alleviation of hemifacial spasm reached its peak in a few days. Availability was 93.3% and its effect lasted about 4 months. Side effects were observed in 12 patients (40%), but no serious complications were observed.

Adult↗

PubMed1991

[The effect of intravenous injection of isosorbide dinitrate on the hypertension during general anesthesia: a multi-center controlled study].

The antihypertensive effect of intravenous injection of isosorbide dinitrate (ISDN) was evaluated in 137 patients undergoing elective surgery during general anesthesia [neuroleptanesthesia (NLA) or enflurane-nitrous oxide-oxygen-anesthesia (GOE)]. ISDN in dose of 20 micrograms.kg-1 or 40 micrograms.kg-1 was given as a bolus injection in 30 sec. ISDN produced a significant decrease in arterial pressure and central venous pressure; the maximum decrease was observed in 7 min after administration of ISDN. The antihypertensive effect of ISDN was dose-dependent, but there was no significant difference between two groups of patients given 20 or 40 micrograms.kg-1, or between those anesthetized with NLA or GOE. ISDN did not significantly alter heart rate, thereby causing a significant decrease in rate pressure product which reflects myocardial oxygen demand. The results suggest that a bolus injection of ISDN is a simple, practical and effective means of controlling hypertension during general anesthesia.

Adult↗

PubMed1991

[Iontophoretic administration of indomethacin in the treatment of postherpetic neuralgia].

Iontophoretic administration of indomethacin (IND) was applied to investigate its effect in 21 cases of postherpetic neuralgia (PHN), which showed no response to other treatment such as nerve blocks. Iontophoretic therapy of IND was applied for 20 minutes once a week up to 5 time (series 1). When pain relief was unsatisfactory in series 1, the same treatment was given once again (series 2). Long term follow-up results were obtained 3 months after the final treatment in series 1 or 2. We used visual analog scale (VAS) to measure their pain intensity. VAS was reduced clearly at the end of the treatment in each series. The average improvement rate of the VAS in each series was as follows; series 1:60.3 +/- 8.2%, series 2:73.7 +/- 7.0%, long term follow-up results: 73.7 +/- 7.0% (mean +/- S.E.) We concluded from the present results that iontophoretic administration of IND was one of the effective and useful treatments for PHN.

Female↗

PubMed1990

Nociceptive effects induced by intrathecal administration of prostaglandin D2, E2, or F2 alpha to conscious mice.

The effects of intrathecal administration of prostaglandins on pain responses in conscious mice were evaluated by using hot plate and acetic acid writhing tests. Prostaglandin D2 (0.5-3 ng/mouse) had a hyperalgesic action on the response to a hot plate during a 3-60 min period after injection. Prostaglandin E2 showed a hyperalgesic effect at doses of 1 pg to 10 ng/mouse, but the effect lasted shorter (3-30 min) than that of prostaglandin D2. Similar results were obtained by acetic acid writhing tests. The hyperalgesic effect of prostaglandin D2 was blocked by simultaneous injection of a substance P antagonist (greater than or equal to 100 ng) but not by AH6809, a prostanoid EP1-receptor antagonist. Conversely, prostaglandin E2-induced hyperalgesia was blocked by AH6809 (greater than or equal to 500 ng) but not by the substance P antagonist. Prostaglandin F2 alpha had little effect on pain responses. These results demonstrate that both prostaglandin D2 and prostaglandin E2 exert hyperalgesia in the spinal cord, but in different ways.

Animals↗

PubMed1990

Isolation of temperature-sensitive CHO-K1 cell mutants exhibiting chromosomal instability and reduced DNA synthesis at nonpermissive temperature.

Twenty-five temperature-sensitive (ts) mutants were isolated from Chinese hamster CHO-K1 cells after mutagenization with N-methyl-N'-nitro-N-nitrosoguanidine. Of 13 complementation groups identified, nine exhibited chromosomal instability at a nonpermissive temperature. They were classified into three major classes according to inducibility of sister chromatid exchange (SCE) and/or chromosomal aberration (CA): class 1 resulted in predominant SCEs, class 2 manifested both SCEs and CAs, and class 3 exhibited higher induction of CAs. Flow cytometric analysis of the mutants exhibiting chromosomal instability indicated that many of the mutants were arrested in the S or S to G2 phases of the cell cycle at the nonpermissive temperature, accompanied by a decrease in the rate of DNA synthesis. These results imply that ts defects are related to some points in DNA replication and might be responsible for the induction of SCEs and/or CAs at the nonpermissive temperature.

Animals↗

PubMed1990

Studies on the enhanced effect of acupuncture analgesia and acupuncture anesthesia by D-phenylalanine (2nd report)--schedule of administration and clinical effects in low back pain and tooth extraction.

D-phenylalanine (DPA) is known to block the activity of carboxypeptidase, an enzyme which degrades enkephalins, endogenous morphine-like substances. Therefore, it is considered that DPA administered as an inhibiting drug of this degrading enzyme might prolong analgesia induced by acupuncture. 1) Thirty patients suffering from chronic low back pain were treated with acupuncture 30 minutes after the oral administration of 4.0 grams of DPA. The results were: excellent in 7 cases, good in 11, fair in 6 and poor in 6. Cases graded excellent and good were then compared with a placebo group. The effect was increased 26% in the DPA-acupuncture group, which shows no statistically significant difference (P less than 0.1). 2) In 56 patients, tooth extraction was performed under acupuncture anesthesia: 18 had received 4.0 gram of DPA (P.O.) 30 minutes earlier. The results were excellent in 8, good in 6, fair in 3, and poor in 1. The excellent and good cases were compared with 38 placebo group cases. The effect in the DPA-acupuncture anesthesia group was significantly increased by 35% (P less than 0.01). 3) In order to determine the optimum time for the administration of DPA, two schedules of administration were compared. [1] DPA was given on the previous day in three 0.5 gram doses (26 cases). [2] A single 4 gram dose was administered 30 minutes before treatment (30 cases). The results from the "excellent", "good" and "fair" cases showed a 16% increase in effectiveness when DPA was administered the day before, not a statistically significant difference (P less than 0.1), but a clear tendency to increase was observed. The above findings show that DPA has an enhancing effect on acupuncture analgesia and anesthesia in clinical practice.

Acupuncture Analgesia↗

PubMed1990

Fidelity of DNA polymerase alpha partially purified from a mutator mutant and wild-type mouse FM3A cells.

Several mutator mutants were isolated from a cultured mouse cell line (FM3A). The mutants exhibited a high rate of spontaneous mutation at three genetic loci of drug resistance. To investigate a possible link between mutator phenotype and fidelity of DNA replication, DNA polymerase alpha was partially purified from the wild-type and the mutator mutant (Fmut 1) showing the highest mutation rate. Using a combination of synthetic template and primer, the ratio of incorporation of incorrect to correct nucleotides was determined. The results indicated that the DNA polymerase alpha from the mutator mutant showed a slightly higher rate of misincorporation, 1.4 and 1.6 times, than that of the wild-type.

Animals↗

PubMed1990

[Usefulness of midazolam for premedication before local anesthesia].

The optimal dose and adverse effects of midazolam for premedication were evaluated in 45 patients undergoing local anesthesia. The patients were divided into 3 groups and administered midazolam intramuscularly: group-A 0.075 mg.kg-1, group-B 0.1mg.kg-1, group-C 0.125 mg.kg-1. In this study, concerning sedative and hypnotic effects, the administration of midazolam (0.075-0.1mg.kg-1) showed satisfactory results except in conduction anesthesia of upper extremities (brachial plexus block). However, severe decrease in PaO2 was observed in relatively younger patients. Therefore, it is necessary to observe patients carefully during perioperative period.

Adolescent↗

PubMed1989

Suppression of deoxyguanosine cytotoxicity and deoxyguanosine kinase activity in mouse FM3A mammary carcinoma cell: mutants defective in hypoxanthine phosphoribosyl-transferase.

The cytotoxic effect of deoxyguanosine was examined in various cell clones isolated from cultured mouse FM3A mammary carcinoma cells. The inhibitory effect of deoxyguanosine on the growth of the wild-type cell was suppressed by the addition of hypoxanthine to the culture medium. Cell mutants defective in hypoxanthine phosphoribosyl-transferase (Hprt- mutants) were approximately 20 times less sensitive to deoxyguanosine. But the sensitivity of the Hprt+ revertants was restored and has become close to that of the wild-type cell. Both uptake and incorporation of [3H]-hypoxanthine and [3H]-deoxyguanosine did not occur in the cellular acid-soluble and insoluble fractions in Hprt- mutants but they were restored in all Hprt+ revertants. When the activities of deoxyguanosine kinase were measured immediately after preparation of the cell-free extracts from the Hprt- mutants, it was negligible but activity comparable to that of the wild-type appeared after dialysis of the extract. These results are explained by assuming accumulation of purine metabolite in Hprt- mutants which inhibited the deoxyguanosine salvage pathway.

Animals↗

PubMed1988

Studies on the enhanced effect of acupuncture analgesia and acupuncture anesthesia by D-phenylalanine (first report)--effect on pain threshold and inhibition by naloxone.

It has been claimed that the mechanism of acupuncture analgesia can be explained in part by endogenous opioids. If so, it might be possible to enhance the analgesic effect of acupuncture by the administration of endorphins. If D-phenylalanine (DPA), an inhibitor of the endorphin degrading enzyme, is administered, the analgesic effect of acupuncture should be prolonged due to the increased level of endorphins. From the changes of the pain threshold (PT), we investigated whether or not the pre-administration of DPA can enhance the analgesic effect of acupuncture in humans. In addition, we examined the inhibitory effect of naloxone. 1) In all five subjects whose PT was raised after acupuncture anesthesia (respondents), the rise in PT was significantly prolonged by DPA. 2) Out of 10 subjects whose PT remained almost unchanged after acupuncture anesthesia (non-respondents), the PT was increased by DPA in 5 cases. 3) The rise in PT was most prominent when DPA was administered 30 minutes before the start of acupuncture anesthesia. 4) In all 4 respondents in whom the rise in PT persisted after DPA and acupuncture anesthesia, their raised PT dropped after the intravenous injection of naloxone (10 mg). 5) These findings show that DPA enhances the analgesic effect of acupuncture by the "endorphin mechanism."

Acupuncture Therapy↗

PubMed1988

A new transfection method of mouse FM3A mammary carcinoma cells with plasmid DNA.

High-frequency transfection of mouse FM3A cells with plasmid pSV2neo DNA was achieved by incubation of the cells with DNA plus polybrene for 6 hours followed by an osmotic shock with hypertonic NaCl solution. When incubated for 20 min at 34 degrees C, FM3A cells showed resistance to the osmolarity change from 0.1 to 9.0% NaCl in the medium. Within this range of NaCl concentration, 5-7% gave the highest efficiency of transfection. Both linear and circular forms of plasmid DNA produced transformants with equal efficiency. This method was simple, reproducible and carrier DNA was not required. The efficiency was about 100 times higher than that of the widely used method with DNA-calcium phosphate precipitates. Transformed cells were stable and different numbers of plasmid DNA copies were detected with different restriction sites.

Animals↗

PubMed1987

High-frequency transfection of mouse FM3A mammary carcinoma cells in suspension culture with plasmid DNA.

High-frequency transfection of mouse FM3A cells, grown in suspension, with plasmid pSV2neo DNA was achieved by incubation of the cells with DNA plus polybrene for 6 h followed by an osmotic shock with a hypertonic NaCl solution. When incubated for 20 min at 34 degrees C, FM3A cells showed resistance to the osmolarity change from 0.1 to 9.0% NaCl in the medium. Within this concentration range, 5-7% gave the highest efficiency of transfection. Both linear and circular forms of plasmid DNA produced transformants with equal efficiency. This method was simple, reproducible, and carrier DNA was not required. The efficiency was about 100 times higher than that of the method with DNA-calcium phosphate precipitates. Transformed cells were stable and different numbers of plasmid DNA copies were detected.

Animals↗

PubMed1986

Alterations in nociception after intracisternal administration of prostaglandin D2, E2 or F2 alpha to conscious mice.

The effect of intracisternal administration of three major prostaglandins on nociceptive responses was evaluated in mice. Prostaglandin D2 had biphasic effects on pain thresholds (hot plate and acetic acid writhing tests) when given in a dosage range of 5 ng to 5 micrograms per mouse. Lower doses of prostaglandin D2 (less than or equal to 15 ng) increased the sensitivity to pain stimulation. Higher doses (greater than or equal to 50 ng) caused hypoalgesia, which was completely blocked by intracisternal injection of 500 pg of naloxone. Prostaglandin E2 (5 ng-5 micrograms) also had a biphasic effect on pain thresholds, similar to the effect of prostaglandin D2. However, the hypoalgesia caused by a higher dose of prostaglandin E2 (5 micrograms) was not blocked at all by naloxone doses of up to 500 ng. Prostaglandin F2 alpha had little effect on pain thresholds. These results indicate that each prostaglandin has a specific effect on the modulation of nociception.

Animals↗

PubMed1986

Isolation of temperature-sensitive mouse FM3A cell mutants exhibiting conditional chromosomal instability.

Fourteen mutants exhibiting conditional induction of sister chromatid exchanges (SCEs) and/or chromosomal aberrations (CAs) were selected out of 65 temperature-sensitive (for growth) mutants from mouse FM3A cells treated with N-methyl-N'-nitro-N-nitrosoguanidine. The mutants with chromosomal abnormality at the nonpermissive temperature were classified into three groups. The group 1 mutant manifested mainly SCEs at the nonpermissive temperature. The group 2 mutants manifested both SCEs and CAs. The group 3 mutants, including ts-131b, a mutant defective in DNA replication and previously described, manifested only CAs. A substantial number of SCEs accompanied by CAs at the site of SCEs were observed in some of the group 2 mutants at the nonpermissive temperature. The results suggest that there are at least three processes involved in the formation of SCEs and CAs, one is common to both phenomena while the others are independent. Some other mutants belonging to the groups 1 and 2 displayed a high incidence of interchromosomal chromatid exchanges at the nonpermissive temperature, suggesting that the same genetic defects leading to high induction of SCEs correlate with induction of chromosomal rearrangements. From these results, the group 2 mutants were subdivided further into five classes, and the mutants in each class had different cytogenetic properties from one another. This indicates defects in at least several genes that participate in the production of SCEs or CAs. The mutants described should be useful for analyzing the mechanisms of SCE or CA formation, and also the mechanism of chromosomal rearrangements.

Animals↗

PubMed1986

Comparison of the effects of leaving needle, direct current electrical acupuncture, and low-frequency electrical acupuncture therapy.

During a 3 year period from 1978 to 1980, one hundred and seventeen patients who came to the Pain Clinic of the Department of Anesthesiology of the Osaka Medical College complaining of pain, were treated with leaving needle(LN), direct current electrical acupuncture (EAP), or low-frequency electrical acupuncture(LFEA). The immediate effects of these therapies were investigated in a period of 3 days after treatment, on the basis of the patient's subjective evaluation of his relief from pain on a scale of 10 before treatment. Appearance patterns of therapeutic effects of these therapies were divided into four types: persistent (therapeutic effects persist during the 3 day period immediately after treatment), downward (therapeutic effects gradually decrease immediately after treatment), upward (therapeutic effects gradually appear after treatment), and unaltered (therapeutic effects were fair or poor during the 3 days after treatment). In addition, it was seen that of the three therapies LFEA was the best one in producing a persistent therapeutic effect and a good rate of effectiveness in the first day immediately after treatment.

Acupuncture Therapy↗