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Biomedical subjects

M Horikawa

Publications and source records attributed to M Horikawa.

At least 109 records · Page 6Linked to original sources
PubMed1976

Effect of stirring during fixation upon immunofluorescence. Results with distribution of albumin-producing cells in liver.

When the immunofluroscent study on the distribution and the incidence of albumin-producing hepatocytes in the rat liver was performed by the method of Sainte-Marie, the number of positive cells showed various values (10-60%). It was surmised that when the permeability of the fixative was delayed, albumin had flowed out from the cytoplasm of the unfixed hepatocytes. By the simple means of constant stirring of the fixative using a magnetic stirrer, we accomplished rapid fixation and achieved results in which positive cells attained 100%. On the other hand, the incidence of positive cells decreased markedly when rats were fed a protein-free diet.

Animals↗
PubMed1976

Fasting therapy for psychosomatic diseases with special reference to its indication and therapeutic mechanism.

It has been well known that the fasting therapy which was invented in Medical School of Tohoku University reveals an excellent effect upon various kinds of psychosomatic diseases, but its therapeutic mechanism and suitable indication are not yet explained completely. In order to corroborate these problems, this study was undertaken on 262 cases of psychosomatic diseases in the field of internal medicine. It is a complete fasting for 10 days with nothing by mouth except for drinking water, and 500 ml of parenteral fluid containing vitamins are administered intravenously every day. Absolute bed rest and self meditation are required in a closed individual room, and patients are not allowed to meet anyone but physicians and nurse in charge. The return to normal ordinary diet follows the order of fluid diet, soft diet and semiordinary diet during 5 days. In the period of the therapy, various clinical and laboratory examinations were carried out. Significance of these examinations consists in prediction of possible danger during the fasting period and elucidation of its therapeutic mechanism. Consequently, an outstanding efficacy rate of 87% with excellent prognosis was attained, and the following diseases were determined as suitable indication of this therapy; irritable colon, dysorexia nervosa, borderline hypertension, neurocirculatory asthenia, bronchial asthma, mild diabetes mellitus, obesity, lumbago without organic findings, conversion hysteria, various neurosis with somatic symptoms and masked depression. Possible mechanism of action of the therapy is that fasting acts as an extreme stress on the function of the autonomic nervous and endocrine systems, then it regulates the function of whole body including the brain, also it acts as one of the behaviour therapy for abnormal conditioning.

Blood Glucose↗
PubMed1975

Analyses of differential sensitivities of synchronized HeLa S3 cells to radiations and chemical carcinogens during the cell cycle. III. 4-Nitroquinoline 1-oxide and its derivatives.

Sensitivity to the chemical carcinogens 4-nitroquinoline 1-oxide (4-NQO) and 4-hydroxyaminoquinoline I-oxide (4-HAQO), during the cell cycle of synchronized HeLa S3 cells, decreases from the late S to the early G2 phases. Cells in other phases are relatively sensitive to both carcinogens. [3-H]4-NQO and [ 3-H]4-HAQO seem to be bound preferably more with cellular DNA of the mitotic phase to the middle of the S phase than with that of the late S phase in which the cells are rather insensitive to these carcinogens. However, we found no significant difference in the excision rates of these carcinogens from the DNA of HeLa S3 cells through the cell cycle. These findings indicate that the cyclic variation of 4-NQO and 4-HAQO cell survivals during the cell cycle may be due to the differences in the amounts of 4-NQO and 4-HAQO bound with cellular DNA.

4-Hydroxyaminoquinoline-1-oxide↗
PubMed1975

Effects of radiopaque material on coronary vascular reserve.

The relationship between the increase in coronary blood flow (CBF) following contrast material injection and that following temporary occlusion was studied in the anesthetized dogs. The peak flow following a contrast injection was almost equivalent to the peak reactive hyperemia flow following a 10 sec occlusion (r equeals 0.86). An increase in coronary sinus blood oxygen pressure (Pcso2) occured in associated with an increase in CBF. Administration of dipyridamole diminished the increment of CBF following contrast injection with little changes in Pcso2. Thus, in the coronary system the increase in CBF after contrast injection, similar to that observed after temporary occlusion, is related to coronary vascular reserve and measurement of Pcso2 after coronary arteriography might be an aid to evaluate the coronary vascular reserve.

Animals↗