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Biomedical subjects

M Honda

Publications and source records attributed to M Honda.

At least 703 records · Page 39Linked to original sources
PubMed1986

Genetic recombination between closely linked markers of bacteriophage T4. II. A mutation which reduces the formation of heteroduplex heterozygotes.

A T4 phage mutant MCO4 was isolated as a mutant that reduced the frequencies of recombinants between multiple closely-linked markers but not those between two markers. MCO4 mutation was a non-lethal, recessive mutation and was located nearby gene 24 and gene 25. MCO4 mutation reduced the frequency of heteroduplex heterozygotes but not the frequency of terminally redundant heterozygotes. Therefore, MCO4 mutation appears to block the formation and/or the extension of heteroduplex regions or to shorten their life-time.

Bacteriophages↗
PubMed1986

[Transfer of cefmenoxime to lung tissue and thoracic muscle in thoracic surgery].

Twenty patients who were performed pulmonary resection for the disease of the lung were administered 2 g of cefmenoxime (CMX) intravenously during the operation. The CMX levels in serum, lung tissue and thoracic muscle were measured by agar-well technique. The CMX levels in lung tissue and thoracic muscle were higher than the MIC80 of CMX for Klebsiella pneumoniae, Haemophilus influenzae and Streptococcus pneumoniae which were commonly as isolated causative organisms from the patients with pulmonary infection. These results indicate that CMX will be useful agent for the prevention and treatment of pulmonary infection.

Adolescent↗
PubMed1986

Genetic recombination between multiple markers of bacteriophage T4. III. A mutation which blocks a structural change of DNA required for strand exchange.

T4 phage mutation MCO5 is a non-lethal recessive mutation which blocks recombination between multiple closely linked markers and links to genes 24 and 25. The three-factor cross, which required double exchange flanking the central marker for the formation of wild type recombinant, was blocked by MCO5 mutation. The blockage was restored by the UV irradiation of the parental phage carrying single mutation but not by the UV irradiation of the parental phage carrying double mutation. The MCO5 mutant reduced the rescue of the markers of UV-irradiated phage, although it had normal levels of sensitivity to UV in both single and multiple infections. From these results, it is concluded that MCO5 mutation blocks a structural change of DNA which is necessary for receiving DNA segment.

Bacteriophages↗
PubMed1986

[A case of synchronous contralateral renal cell carcinoma and ureteral transitional cell carcinoma].

A case of synchronous contralateral renal cell carcinoma and ureteral transitional cell carcinoma is reported. A 56-year-old man, who had been incidentally found to have an abnormal mass in the upper pole of the right kidney on ultrasound sonography, was admitted on January 8, 1985. CT scanning and renal arteriography revealed right renal malignancy. Right radical nephrectomy was performed and histological examination showed adenocarcinoma, granular cell type of the right kidney. He was discharged on February 3, 1985. Two months postoperatively, he was rehospitalized for macroscopic hematuria. Left retrograde pyelogram showed obstruction at middle ureter and cytology of urine from left ureter was positive. So a left ureteral tumor was suspected, and partial resection of left ureter and ureteroureterostomy were performed. Histological examination revealed ureteral transitional cell carcinoma. He is now doing well at 6 months following the lat surgery, without any evidence of recurrence.

Carcinoma, Renal Cell↗
PubMed1985

Production and characterization of a unique monoclonal antibody against human B cells (33.2.1).

A monoclonal antibody specific for a polymorphic antigen on human B cells (33.2.1) was produced and characterized. By flow cytometry, 33.2.1 was found to react with peripheral blood B cells, monocytes, and Epstein-Barr virus (EBV)-transformed B-cell lines, but not with peripheral blood T cells, mitogen-activated T cells, or allo- or autoactivated T cells. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that 33.2.1 recognizes a noncovalently bound bimolecular complex composed of an alpha chain of about 32 kDa and a beta chain of about 28 kDa. The failure of anti-HLA-DR, anti-Leu-10, and anti-HLA-DC1 to remove the 33.2.1 antigen by sequential immunoprecipitation suggests that 33.2.1 recognizes a distinct molecule rather than a different epitope on either HLA-DR or DS/DC/MB. In T-cell-independent B-cell activation systems, preincubation with 33.2.1 markedly inhibited RNA and DNA synthesis as well as polyclonal Ig production. In contrast, anti-HLA-DR was inhibitory only when it was present throughout the culture, but not when it was used for preincubation. Anti-Leu-10 led to only moderate inhibition. These results suggest that 33.2.1 recognizes a unique Ia-like antigen critical for B-cell activation.

Animals↗
PubMed1985

Effect of TSH on conversion of T4 to T3 in perfused rat kidney.

This study was undertaken to elucidate the effect of thyrotropin (TSH) on the conversion of thyroxine (T4) to 3,5,3'-triiodothyronine (T3) in the isolated perfused rat kidney. The kidney was perfused with a synthetic medium containing 20 micrograms/dL T4 and the effect of constant infusion of bovine TSH (125 or 250 microU/mL) on the conversion of T4 to T3 was investigated. T4 uptake in the perfused kidney was not changed by the addition of TSH. However, the release of T3 (89 +/- 11 ng/g/30 min, mean +/- SD), tissue T3 (190 +/- 23 ng/g/30 min), net T3 production (227 +/- 37 ng/g/30 min), and the conversion rate of T4 to T3 (9.5 +/- 1.6%) in the kidney perfused with 250 microU/mL TSH were significantly (P less than 0.005) greater than those in controls (63 +/- 9, 143 +/- 15, 152 +/- 36 ng/g/30 min, and 6.3 +/- 1.9%), respectively. Degradation rate of T3 in perfused rat kidney was not changed by the addition of 250 microU/mL TSH. These results suggest that TSH may directly affect renal iodothyronine-monodeiodinating activity in rats in vitro.

Animals↗
PubMed1985

Non-Hodgkin's lymphomas in Osaka, Japan.

Six hundred and eight-four cases with non-Hodgkin's lymphomas (NHL) in Osaka, Japan were reviewed, using current histopathological classification to obtain a general feature of Japanese lymphomas. There were no remarkable differences in the distribution of age, sex and the location of primary tumors between Japanese NHL and the disease in Western races. Histologically, however, several differences were present; in particular, the lower frequencies of nodular lymphomas of nodal origin and lymphocytic type of nodal and extranodal origin in the present cases. About 10% of the cases show a peculiar histology characteristic for adult T cell lymphomas. From this study, it appears that the ratio of low-grade malignant NHL in Japan is much lower than in the Western countries.

Adolescent↗
PubMed1985

Mediation of macrophage reactions in immune tissue injury.

The chemotactic specificity of three types of macrophage chemotactic factors (MCF) -a, -b, and -c, from delayed hypersensitivity reaction (DHR) skin sites in guinea pigs, was analysed. MCF-c shared common antigenicity with the macrophage chemotactic lymphokine released from bovine gamma globulin (BGG) and horse radish peroxidase (HRPO) -stimulated lymphocytes, using an immunoadsorbent column conjugated with anti-MCF-c antibody. These purified lymphokines were very similar, or possibly identical in terms of physicochemical and serological properties. BCG-induced lymphokine seemed to exist as complexes with serum protein at the skin site. A change in the proportion of each MCF was observed during the development of DHR. Furthermore, MCF-a and -b attracted Ia- M1 cell line cells, while MCF-c attracted Ia+ cells. Moreover, the responsive guinea-pig monocytes were divided mainly into two distinctive migrating subpopulations. One subpopulation was responsive to MCF-a and -b, and the majority of responding cells were "Ia-negative". The second subpopulation was responsive to MCF-c and the predominant cell type was "Ia-positive". The data suggest that macrophage reactions in the DHR are mediated by MCF-a, -b, and -c and that MCF-c attracts Ia bearing accessory macrophages and MCF-a and b- attract Ia negative macrophages.

Animals↗
PubMed1985

Effects of purified hog pancreatic kallikrein on the kinin-prostaglandin system and renin-angiotensin-aldosterone system.

In the present study, hog pancreatic kallikrein (HPK, 2,000 KU/kg body weight) was intramuscularly injected into male rabbits and several plasma hormones [kinin, prostaglandin E (PGE), 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha), thromboxane B2 (TXB2), plasma renin activity (PRA), aldosterone, and ACTH] were measured before and after the HPK administration, in order to clarify the role of glandular kallikrein in the blood. The plasma kinin concentrations were significantly (p less than 0.001) increased from 1 +/- 1 (mean +/- SE), the baseline level, to 230 +/- 22, 288 +/- 36 and 130 +/- 9 pg/ml at 30, 60, and 120 min, respectively, after HPK administration. The plasma level of PGE were slightly increased after HPK administration, but the change was not significant compared to the mean baseline level. The plasma levels of 6-keto-PGF1 alpha were significantly increased from 229 +/- 38, the baseline level, to 594 +/- 131 (p less than 0.05) at 30 min after the administration of HPK and tended to increase (378 +/- 67) at 60 min after the HPK administration, but fell to 278 +/- 37 pg/ml at 120 min after the administration of HPK. On the other hand, the changes in plasma TXB2, aldosterone, ACTH, and PRA before and after HPK administration were not significant. The present results showed that exogenous intramuscular administration of HPK increased the plasma levels of kinin and prostacyclin, but caused no elevation in the plasma levels of other hormones including PRA. It is concluded, therefore, that in this acute experiment, there was a close relationship between the kallikrein-kinin system and prostaglandins.

6-Ketoprostaglandin F1 alpha↗
PubMed1985

Studies of the pathogenesis of angioimmunoblastic lymphadenopathy.

We studied the immune functions of two patients with angioimmunoblastic lymphadenopathy (AILD) in an attempt to determine whether the B cells were primarily hyperactive or, rather, if T cell abnormalities might underlie the B cell hyperactivity observed in these patients. We found that the B cells of the AILD patients did not proliferate spontaneously, nor were they induced to proliferate excessively by fresh normal T cells. In contrast, AILD T cells induced both autologous and allogeneic B cells to proliferate and to differentiate into Ig secreting cells. Spontaneous culture supernates of T cells obtained from each patient induced substantial proliferation of B cells (B cell-activating activity) as well as proliferation in a standard costimulatory assay (B cell growth factor activity). The culture supernate of a T cell line, which was established from one patient, showed both activities. The T cell line supernate also induced Ig production by staphylococcal A Cowan-activated B cells. None of these properties of AILD T cells was found among 10 normal controls. The addition of AILD T cells to autologous or allogeneic B cells in the presence of pokeweed mitogen (PWM) led to marked suppression of both proliferation and Ig production. This was true even in the presence of fresh normal T cells. Pretreatment studies showed that suppressor cells were induced by the interaction of AILD T cells with PWM-activated B cells. The present study suggests that the B cell hyperactivity observed in AILD patients might in part be due to excessive T cell effects on B cells. In addition, our results may help clarify the paradoxical impaired responsiveness to in vitro stimulation with PWM by active B cells from patients with autoimmune diseases.

Antibodies, Monoclonal↗
PubMed1985

[Malignant lymphoma of the testis: report of two cases].

Two cases of malignant lymphoma arising from the testis are reported. One was 45 years of age, with the complaint of left scrotal swelling with dull pain and the other was 68 years of age, with the complaint of painless swelling of right scrotal contents. Microscopic findings were both malignant lymphoma of the testis, large cell and immunoblastic type in case 1 and diffuse mixed, small and large cell type in case 2 (Working Formulation, 1982). Biomedical and radiographic examinations revealed no apparent involvement otherwise. For the period of 6 months in case 1 and 2 months in case 2 following radical orchiectomy with postoperative irradiation (Lineac 4,000 rad), the patients have been doing quite well without any clinical evidence of recurrence. The literature is also reviewed briefly.

Aged↗