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Biomedical subjects

M Hishiyama

Publications and source records attributed to M Hishiyama.

43 records · Page 3Linked to original sources
PubMed1984

Inhibition of host cellular ribonucleic acid synthesis by glycoprotein of mumps virus.

After infection with mumps virus, cellular ribonucleic acid synthesis of a murine lymphoma cell line, EL4, was appreciably depressed. The inactivation of viral infectivity by ultraviolet irradiation or the treatment of cells with mouse interferon did not abolish the inhibiting effect, suggesting that virus replication is not required for the depressed RNA synthesis. Envelope glycoproteins isolated from disrupted mumps virus caused inhibition of cellular RNA synthesis. The addition of low concentrations of specific antibody enhanced the inhibitory effect, probably through the formation of aggregates of glycoproteins. On the contrary, the glycoproteins showed no effect on RNA synthesis in the presence of cytochalasin D.

Animals↗
PubMed1984

Abortive infection of mumps virus in murine cell lines.

Infection of L929 or BW5147 cells with mumps virus was shown to result in an abortive type of infection in that little or no progeny virus was produced. Both cell lines could adsorb mumps virus, indicating that restricted viral growth in these cells was not attributable to the absence of mumps virus receptors. Using [35S]methionine-labelled virus, it was demonstrated that restriction of virus growth in BW5147 cells was due in part to inefficient virus penetration into the cells. Virus-specific polypeptides were synthesized in mumps virus-infected L929 cells but were not detected in infected BW5147 cells. After addition of actinomycin D or anti-interferon serum to the cultures, mumps virus was able to replicate in L929 cells, whereas no virus growth was apparent in BW5147 cells.

Animals↗
PubMed1984

Enzyme-linked immunosorbent assay compared with neutralization tests for evaluation of live mumps vaccines.

Mumps-specific antibody levels before and after vaccination with live mumps vaccines were determined by enzyme-linked immunosorbent assay (ELISA) and neutralization tests. A correlation was found between neutralization titers and optical density in ELISA. However, postvaccination sera from some vaccinees who failed to seroconvert by neutralization contained significant levels of mumps-specific antibody detectable by ELISA. In some of these serum specimens, the antibody directed to the F polypeptide of mumps virus was predominant. Most sera positive in ELISA neutralized mumps virus upon the addition of fresh guinea pig serum to the virus-serum mixture.

Antibodies, Viral↗
PubMed1984

Interferon induction in mouse spleen cells by mitogenic and nonmitogenic lectins.

Twenty-two species of lectin were tested for their ability to induce interferon (IFN) in mouse spleen cells. Twenty-two species of lectins representing four groups, based on competition patterns with monosaccharides, were examined for their ability to induce IFN in cultured mouse spleen cells. The lectins, all belonging to the third group (concanavalin A, succinylated concanavalin A, Lens culinaris lectins type A and B, and poke weed mitogen) induced IFN mainly composed of IFN gamma. They were either T cell or T/B cell mitogens. Five nonmitogenic lectins, Lotus tetragonolobus seed lectin, crude and type II lectins of Ulex europeus, Bandeiraea simplicifolia type II and Salanum tuberosame lectins, and wheat germ agglutinin belonging to either the first or the third group, induced IFN beta. The production of IFN during stimulation IFN beta- and IFN gamma-inducing lectins followed different kinetic curves. WGA induced IFN in circulation when injected i.p. in mice, and a peak titer was found 2 hr after inoculation.

Animals↗
PubMed1979

Continuous lines of RSV-transformed embryo cells and peritoneal macrophages of Japanese quails.

Four continuous lines of RSV-transformed quail cells were established; QERC-31F and QERC-31N cells derived from quail embryo cells and PERP and PERY cells from adult quail peritoneal macrophages. Marked morphological difference was noted between QERC-31F and QERC-31N; the former showed fusiform shape and the latter nodular shape. Both PERP and PERY showed macrophage-like morphology with phagocytic capacity. All four cell lines contained gs antigen and gp 85. Production of transforming virus was found in QERC-31N, PERP and PERY. In spite of failure in production of transforming virus, EQRC-31F was demonstrated to produce C-type particles by electron microscopy and to contain tumor-specific surface antigen by in vivo immunization and in vitro microcytotoxicity tests.

Animals↗
PubMed1978

Lysis of RSV-transformed Japanese quail cells by a factor from normal quail serum.

Normal sera of Japanese quails caused lysis of tumor cells from a Rous sarcoma virus (RSV)-induced quail tumor (QT cells). Other tumor cell lines, including RSV-transformed quail embryo cells and methylcholanthrene-induced quail tumors, were not lysed. This naturally occurring cytolytic factor (NCLF) was sensitive to heating at 56 degrees C, zymosan, and inulin, and it required magnesium but not calcium for the expression of its activity. These results suggested that NCLF activity was mediated by complement activated through an alternative pathway. This possible complement activation occurred in the absence of specific antibodies to the target cells.

Agammaglobulinemia↗