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Biomedical subjects

M Hidaka

Publications and source records attributed to M Hidaka.

142 records · Page 8Linked to original sources
PubMed1985

[Combined resection of the thoracic aorta associated with subtotal esophagectomy for carcinoma of the esophagus].

With recent advances in surgical techniques and post-operative care, surgical treatment of esophageal cancer has been safely performed. Nevertheless, prognoses of such cases in which the cancer is invading the ajacent aorta and when only an incomplete resection was performed are very poor. The following case in which annular resection of the thoracic aorta combined with subtotal esophagectomy was performed and an aorto-aortic bypass graft was replaced was reported. A 70 year old man came to our hospital, complaining of difficulty in swallowing. The upper GI series showed a 8 cm long filling defect in the middle esophagus. Computed tomography suggested that the tumor was directly invading the aorta. The patient underwent two staged procedure operations. During the first operation, the left chest was opened, and a side-to-end bypass graft was replaced to detour around the tumor bearing aorta with 20 mm phi Dacron Double Velour graft. The aorta was then transected. The second operation was performed through the right thoracotomy. During the operation, the thoracic esophagus and the tumor bearing aorta were resected en bloc. The patient died of hepatic failure and right pyothorax 4 months after the first operation.

Aged↗
PubMed1982

Expression of cloned calf prochymosin gene sequence in Escherichia coli.

An expression plasmid for calf prochymosin (prorennin) cDNA was constructed. The plasmid (pCR301) contains the lacUV5 promoter in front of the fused gene in which the codons for the N-terminal four amino acids of prochymosin cDNA were replaced with those for the N-terminal ten amino acids of beta-galactosidase. Synthesis of the fused protein with the expected Mr was detected immunologically in Escherichia coli harboring pCR301. The product seemed to be localized in the cell membrane of the bacterial host.

Amino Acid Sequence↗
PubMed1982

Nucleotide sequence of calf prorennin cDNA cloned in Escherichia coli.

The nucleotide sequence of prorennin (prochymosin) cDNA cloned in E. coli was determined by the technique of Maxam and Gilbert. The longest prorennin cDNA insert in pTACR1 contained the putative signal sequence and the coding sequence for the peptide from the 1st amino acid, Ala (NH2 terminal), to the 296th, Ser, and the other clone pTACR9 contained the coding sequence from the 258th, Asp, to the 365th, Ile (COOH terminal), and the TGA termination codon followed by the 3'-untranslated region. Thus, the whole coding sequence for prorennin was obtained in the pair of pTACR1 and pTACR9.

Amino Acid Sequence↗
PubMed1981

Cloning in Escherichia coli of the structural gene of prorennin, the precursor of calf milk-clotting enzyme renin.

Double-stranded cDNA was prepared from prorennin-specific mRNA by sequential actions of reverse transcriptase, DNA polymerase and S1 nuclease, and inserted into the Sa/I site of pBR322 by the poly(dG)-(dC) annealing method. Transformation of Escherichia coli C600 r- m- by the hybrid plasmid yielded transformants containing prorennin cDNA. The presence of the cDNA sequence in these clones was confirmed by both colony hybridization and hybrid-arrested translation of the mRNA in vitro. The largest size of the cloned cDNA was 1,020 bp.

Animals↗
PubMed1976

Cell proliferation stimulating factors in the supernatant of embryos and adult muscles of chickens of the same strain.

Stimulating factors of cell proliferation were extracted from the supernatant of embryos and adult muscles of chickens of the same strain. The stimulating activity and physicochemical properties of these factors were altered after embryological development. The embryonic factors were about 3.5 times more effective than the muscular factors. These factors might be useful in analysis of the regulatory mechanisms of cell proliferation.

Animals↗
PubMedDate not supplied

Expression of cloned calf prochymosin cDNA under control of the tryptophan promoter.

To increase yields of calf prochymosin (PC) produced in Escherichia coli, PC cDNA was cloned in a plasmid vector under control of the trp promoter. The hybrid plasmid pCR501 constructed for this purpose contains cDNA coding for PC (from the 5th Arg to the C-terminal Ile) fused to the N-terminal fragment of the trpE gene preceded by the trp promoter and attenuator region. E. coli C600 harboring this plasmid produces approx. 300 000 molecules of PC per cell. This is about a tenfold increase above the amount obtained using lacUV5 promoter [Nishimori et al., Gene 19 (1982) 337-344]. A similar plasmid, pCR601, which contains the same coding sequence fused to the trp promoter and N-terminal fragment of the trpL gene, directs the production of PC at the same rate as pCR501. In pCR601 the trp attenuator is deleted. Another plasmid, pCR701, in which construction of a sequence coding for fMet-PC cDNA that was aided by chemical synthesis, was placed under direct control of the trp promoter, produced PC at a much lower rate. Extracts prepared from all these bacterial transformants in the presence of urea showed distinct milk-clotting activity after renaturation and processing.

Amino Acid Sequence↗