Affinity precipitation of proteins using bis-dyes.
Based on the principle of affinity precipitation of nucleotide-dependent enzymes using bis-NAD (Mosbach), Lowe et al. have recently mentioned the possibility of synthesising bis-dyes for similar applications. In this paper we report preliminary results obtained using bis-dyes in the sulphonamide form got through carbodiimide condensation of the monomer and its aminohexyl derivative for affinity precipitation. The dimer exhibited considerable selectivity for lactate dehydrogenase (90% yield). Bovine serum albumin gave a lower yield of 50% and as expected chymosin could not be precipitated by the dimer.
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