[Pathogenesis, physiopathology, and epidemiology of diabetic microangiopathy].
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Biomedical subjects
Publications and source records attributed to M Hautecouverture.
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Overnight fasting venous plasma glucose concentration was lower in premenopausal women than in men. This difference increased during a low caloric and low carbohydrate diet and above all after a 48 h fasting period. Venous blood glucose variation during this period of fasting was the same in post-menopausal women as in men. These facts and the previous observation by our group of a fall in fasting and post absorptive venous blood glucose after intramuscular injection of oestradiol benzoate (10) indicate that sex variations in the blood glucose fall following a period of fasting are at least partly linked to the oestrogenic status of the premenopausal woman.
The in vitro perifusion technique of rat pancreatic tissue has been studied in respect of sensitivity and reproducibility regarding alpha-2-cell function. Convenient and quickly performed, it allows the kinetic analysis of humoral factors of glucagon release when the blood vessels are too small to be cannulated, for instance in case of fetal pancreas and of tumor tissue. A2 cells in the perifused pancreatic tissue release glucagon in response to arginine, norepinephrine and the decrease of glucose concentration in the medium. The sensitivity of this preparation is lower than that of the perfused pancreas: this may be related to unphysiological conditions of fluid circulation, of the degradation of the stimuli and degradation of the hormone released. Satisfactory conditions of perifusion flow rate and amount of panceatic tissue have been defined and several protective agents have been tested.
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Glucocorticoids increase the stocks of carbohydrate and the supra-hepatic flow of glucose. Their excess produces a centripetal redistribtution of adipose stocks and potentialises adipocyte lipolysis. Although their role of glucose and lipid metabolism is evident, the mechanisms and sites of action of glucocorticoids are multiple and not entirely clear. The actions of glucocorticoids become clear in cases of insulin deficiency, especially when the deficiency is marked, which is of great interest in current medical practice.
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