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Biomedical subjects

M Hassan

Publications and source records attributed to M Hassan.

At least 181 records · Page 10Linked to original sources
PubMed1989

Pattern of obesity and insulin, glucagon, sex hormone binding globulin and lipids in obese Arab women.

A standard oral glucose tolerance test was performed in 86 healthy premenopausal obese Arab women (BMI greater than or equal to 30) Glucose, insulin and glucagon were measured at 0, 30, 60, 90 and 120 min. Sex hormone binding globulin (SHBG), plasma lipids and uric acid were also estimated. Waist-hip circumference ratio (WHR) had significant positive correlation with age, triglycerides (TG), uric acid, fasting and 120 min glucose, and 120 min insulin and significant negative correlation with SHBG. Body mass index (BMI) had significant correlation with uric acid, fasting and 120 min insulin, and significant negative correlation with high density lipoprotein cholesterol (HDL Chol). When separated in two subgroups, with WHR greater than 0.80 (41), and less than or equal to 0.80 (45 cases), plasma glucose was in the diabetic range in seven; and impaired glucose tolerance (IGT) in 11 women in the former subgroup. Only three with IGT but no diabetics, were in lower WHR subgroup. WHR in diabetics (0.93), and in IGT cases (0.90) was significantly higher than in other women (0.80). Fasting insulin was not different, but at 90 and 120 min, insulin was higher in the high WHR subgroup who had also higher fasting, 90 and 120 min glucose. Glucagon level, though slightly higher in the higher (WHR) subgroup, may indicate relative hyperglucagonaemia because of the associated significantly higher glucose. Compared with age matched non-obese controls, obese women in both subgroups had significantly higher insulin, uric acid and significantly lower HDL Chol and lower glucagon (insignificant). Obese women in the higher WHR subgroup (greater than 0.80) had also significantly higher systolic blood pressure, TG and lower SHBG.

Adult↗
PubMed1988

Cloning, nucleotide sequence and molecular evolution of a rabbit processed metallothionein MT-2 pseudogene.

A rabbit metallothionein-2 pseudogene (MT-2 psi) has been isolated from a partial rabbit genomic library. Its unusual sequence shows evidence of complex rearrangements involving recombination and deletion events. There are no intervening sequences, 3' poly A tract or 5' regulatory DNA sequences. The pseudogene is flanked by two sets of direct repeats (CT)3 GT(CT)4 and CTGG(G)CTC. They are most probably the sites of insertion of MT-2 psi in the rabbit genome. In addition, a number of repetitive DNA sequences are observed flanking the MT-2 psi gene. These are features of a processed retrogene.

Amino Acids↗
PubMed1988

Cloning, nucleotide sequence and characterization of a New Zealand rabbit metallothionein-I gene.

We have isolated a rabbit metallothionein-I gene from a lambda gt10 library. The coding sequence of this gene is interrupted by two introns occurring at amino acid positions 9 1/3 and 30 1/3. Comparison of the promoter sequence of this gene with the promoters of other metallothionein genes identified a number of oligonucleotide sequences which are recognized by trans-acting proteins involved in the regulation of these genes by heavy metals, glucocorticoids and alpha interferon.

Amino Acid Sequence↗
PubMed1988

The preparation of a new 57cobalt-labelled thyroxine for use in single/dual radioimmunoassay techniques.

The preparation of L-thyroxine labelled with 57cobalt for use as a second tracer in dual radioimmunoassay techniques for different biological molecules is described. The labelling procedure was monitored by paper chromatography and polyacrylamide gel electrophoresis; a yield of 42 +/- 10% (mean +/- 1 SD) was achieved in a total of 39 conjugation reactions. The method was optimized in terms of pH, reaction completion time, reactant concentration, and solubility of the tracer. A radioimmunoassay for thyroxine in human serum was developed utilizing this tracer, and this compared favorably with an established technique in which 125iodine was used as the tracer (n = 71, r = 0.91, y = 0.988 x - 1.93).

Antigen-Antibody Reactions↗
PubMed1988

Acid-induced esophagitis in cats is prevented by sucralfate but not synthetic prostaglandin E.

The cytoprotective effects of liquid sucralfate and a synthetic analog of prostaglandin E1 (PGE1) on acid-induced esophagitis in cats were studied. Esophagitis was induced in adult cats using a constant infusion of 0.1 N HCl at 1 ml/min for 20 min. Animals were infused for either one or three days. Mucosal lesions were evaluated by blinded investigators using both fiberoptic endoscopy and light microscopy. Histologic changes included basal cell hyperplasia, intraepithelial leukocytosis, and subepithelial leukocytosis. Liquid sucralfate given prior to acid infusion consistently prevented acid-induced lesions in both one- and three-day infusions, demonstrated by both endoscopy and quantitative histologic scoring. Indomethacin (200 micrograms/kg) given prior to sucralfate and acid did not affect sucralfate cytoprotection. Synthetic PGE1, given in doses of 5 micrograms/kg and 100 micrograms/kg, afforded no esophageal cytoprotection. These studies indicate that sucralfate is cytoprotective against acid-induced esophageal injury in cats, an effect that does not appear to be mediated by prostaglandin. In addition, synthetic PGE1 does not confer protection in this animal model.

Animals↗
PubMed1988

Cost of capital to the hospital sector.

This paper provides estimates of the cost of equity and debt capital to for-profit and non-profit hospitals in the U.S. for the years 1972-83. The cost of equity is estimated using, alternatively, the Capital Asset Pricing Model and Arbitrage Pricing Theory. We find that the cost of equity capital, using either model, substantially exceeded anticipated inflation. The cost of debt capital was much lower. Accounting for the corporate tax shield on debt and capital paybacks by cost-based insurers lowered the net cost of capital to hospitals.

Capital Expenditures↗
PubMed1988

Deprecating the diagnostic significance of free thyroxine levels in human serum.

Prime diagnostic importance has been postulated for the measurement of free thyroxine in human serum. During the past decade various innovative and routinely applicable techniques have been developed for its assay, and claims for their superior performance in clinical practice have subsequently been made. However, in our opinion, based on current literature concerning thyroxine metabolism together with basic information about the physicochemical characteristics that govern its intravascular distribution, it seems appropriate to indicate caution against overestimating the diagnostic sensitivity attributed to free thyroxine measurement irrespective of assay methodology and its conceptual elegance.

Humans↗
PubMed1988

Heterogeneity of serum basal C peptide levels amongst Arab patients with diabetes mellitus.

Thirty-four Arab patients with history of diabetes mellitus but without history of ketoacidosis (Group 2), and 16 Arab patients with history of diabetes mellitus and ketoacidosis (Group 1) were studied. They were compared with 15 healthy control subjects and were further broken down according to sex and type of therapy. Fasting serum C-peptide, glucose and glycosylated haemoglobin levels were measured in all subjects. C-peptide levels in Group 2 were higher (t = 2.95) and exhibited greater heterogeneity (Variance ratio 3.60, p less than 0.005) than those in Group 1. Female subjects in Group 2 were more obese than male subjects in the same group, and exhibited greater heterogeneity of their C-peptide levels (Variance ratio 9.47, p less than 0.001). Although male patients in Group 2 on insulin therapy were older than male subjects on diet or oral hypoglycemic agents (t = 3.52, p less than 0.01), female subjects on insulin therapy were younger than those on diet or oral hypoglycemic agents (t = 2.05, p less than 0.05). Furthermore female patients on insulin therapy had lower serum glycosylated haemoglobin levels than female subjects on oral hypoglycemic agents (t = 2.05, p less than 0.05) but male patients on insulin therapy had higher serum glycosylated haemoglobin than those on oral hypoglycemic agents. These data suggest the earlier tendency of physicians to prescribe insulin in women of child bearing age but suggest that earlier prescribing habits of insulin may improve diabetic control.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1988

[Isolated traumatic rupture of the gallbladder. Apropos of 2 cases].

Two cases of isolated traumatic rupture of gallbladder are reported, including results of ultrasound and CT scan imaging, and the relevant literature reviewed. Preoperative diagnosis is suggested by presence of a perivesicular collection. Fine needle puncture aspiration of the collection provides confirmation of diagnosis if a bilious or biliohemorrhagic fluid is withdrawn.

Accidents, Traffic↗
PubMed1988

[Radiological manifestations of splenic and gastroduodenal sarcoidosis. Apropos of 2 cases].

Two cases of abdominal sarcoidosis with radiologic signs are presented. One patient had signs of a calcified splenic pseudo-tumor without other evidence of sarcoidosis, the other a typical pulmonary lesion with parietal infiltration of duodenum on esophagogastroduodenal follow-through radiography. Radiologic forms of splenic and gastroduodenal sarcoidosis reported in the literature are reviewed.

Adult↗
PubMed1988

[Ultrasonic diagnosis of non-calculous intraluminal obstruction in the extrahepatic bile ducts. Apropos of 8 cases].

Ultrasound examination in 8 patients with occlusive jaundice demonstrated non-lithiasic extrahepatic intracanalar obstacles, a malignant tumor in 4 and a non-tumoral process (granuloma on suture thread, ball sludge, hemobilia in 2 cases) in the other 4. Analysis of echographic data suggested possible differential diagnosis criteria for these two types of occlusion.

Adult↗
PubMed1987

Solid tumor preparation for clinical application of flow cytometry.

Intense interest in advanced squamous cell cancers of the head and neck (SCC of H&N) has resulted from the recent progress made in tumor responses with chemotherapy and radiotherapy. Unfortunately, the response patterns and clinical outcome of such patients are not adequately predicted on an individual patient basis using clinical parameters or conventional morphology. The study of flow cytometrically determined cellular parameters in such tumors is therefore of interest, but is hindered by inadequate tumor preparative technology. The previous article (10) in this journal describes the use of a murine SCC tumor, LC12, which was employed for comparative testing and determination of optimum techniques of preparation for this tumor. This report describes the application of these techniques to 144 specimens of human SCC of H&N. The mean total yield for these specimens is 7.4 X 10(7) cells/g of tissue. The mean viable enzymatic yield (3.3 X 10(7) cells/g) was higher than the mean viable mechanical yield (2.0 X 10(7) cells/g) except when lymph nodes were the source of the specimen (5.4 X 10(7) cells/g). The mean dye exclusion viability from enzymatically dissociated specimens were above 90%. Significant aneuploidal subpopulation losses were evident in mechanically dissociated and enucleated specimens. 65% of the enzymatically dissociated specimens were successfully cultured with a mean cloning efficiency of 2.1 X 10(-3). Preparative techniques derived from comparative testing with a murine standard tumor have been successfully applied to 144 specimens of SCC of H&N with resultant high yields and excellent viability. Technical problems detected during the preliminary testing with LC12 were confirmed in the human tumors.

Adult↗
PubMed1987

Phenylpiperazine-based radiopharmaceuticals for brain imaging. 3. Synthesis and evaluation of radioiodinated 1-alkyl-4-phenylpiperazines.

As part of our program in radiopharmaceutical chemistry we have prepared and evaluated a series of radioiodinated 1-alkyl-4-phenylpiperazines as potential brain-imaging agents. The compounds were chosen on the basis of their synthetic versatility, activation toward electrophilic substitution, and ease of purification. The intermediates 1-6 were readily obtained and converted to the corresponding radioiodinated products 7-12 in 76-91% isolated radiochemical yields. The tissue distribution in rats indicated that the 1-N-butyl derivative 9 possesses the best combination of brain uptake (0.28-0.35% ID X kg/g), retention, and selectivity (brain/blood greater than 20) over the 4-h evaluation period. A subsequent imaging and tissue distribution study in the dog using 131I-labeled 9 supported the results observed in the rat and suggested the potential of this agent as a brain-imaging agent.

Animals↗
PubMed1987

Cannulation of the radial and dorsalis pedis arteries.

The complications inherent in cannulation of the radial (group I) and dorsalis pedis (group II) arteries were studied in 46 patients. All the local and systemic variables which might be considered to contribute to damage of the artery were studied, from which it appeared that only the duration of cannulation, prothrombin time and partial thromboplastin time were significant in determining post-cannulation blood flow. A 20-gauge Teflon cannula was used in all patients and velocity of flow measured with a Doppler velocimeter probe. A majority of arteries showed a reduction of flow velocity for 24-72 h after the removal of the cannula, with evidence of improvement at 120 h. In six arteries--three in each group--flow ceased altogether after 24-72 h. No disability of either hand or foot resulted.

Adult↗