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Biomedical subjects

M Hamza

Publications and source records attributed to M Hamza.

At least 109 records · Page 6Linked to original sources
PubMed1985

Automated immunoturbidimetric assay of serum apolipoprotein A-II using the Cobas-Bio centrifugal analyser: influence of hyperlipoproteinemia.

A quantitative automated immunoturbidimetric procedure for the analysis of apolipoprotein A-II (Apo A-II) in human serum is described. Dilution of antibody with a 5% solution of PEG 6000 enhanced the quantification. The within- and between-assay coefficients of variation were less than 5%. Results correlated well with those obtained by classic electroimmunodiffusion and immunonephelometry. No extraction of samples with organic solvent was necessary, whatever the triglyceride concentration. Large lipoproteins such as VLDL and immunocomplexes did not affect the method, nor was there interference from icteric or hemolyzed serum or from serum with excessive hyperlipemia. Physiological values of Apo A-II were determined in a normal population. Concentrations were found to be age-dependent, and higher in women than in men. The procedure is very suitable for the rapid, precise, reproducible and inexpensive assay of Apo A-II.

Adult↗
PubMed1985

[Determination of serum apolipoproteins A1 and B by immunoturbidimetry using the Cobas-Bio centrifugal analyzer. Influence of hyperlipemia].

The authors describe an immunoturbidimetric technique for the determination of the serum apolipoproteins A1 and B using a Cobas-Bio centrifugal analyser. This methods is sensitive and reproducible (CV less than 5%) and show a good correlation with electroimmunodiffusion which was taken as method of reference. It may be applied to serum stored at 4 degrees C or frozen at -20 degrees C. No light is transmitted in the case of either turbid hyperlipemic sera or sera extensively icteric or hemolyzed. The physiological values found in a large population of men and women of various ages are in agreement with those reported in the literature. The method described thus provides a suitable means of investigation adapted to large series for screening for coronary disease states.

Adult↗
PubMed1985

[Pharyngeal stenosis in a case of Behçet's disease].

A case of pharyngeal stenosis in Behcet's disease is reported. A parallelism is done with other systemic disorders such as lupus erythematosus and Wegener's disease. Vasculitis and localised myositis are incriminated in the mecanism of the pharyngeal stenosis.

Adolescent↗
PubMed1983

[Mixed connective tissue disease (Sharp syndrome) with involvement of the trigeminal nerve and facial paralysis].

The authors report a case of mixed connective tissue disease with involvement of the fifth and seventh cranial nerves. While involvement of the trigeminal nerve is well-known, that of the seventh nerve is much less so. However, seventh nerve involvement is beginning to be recognized in other connective tissue diseases such as scleroderma. Neurological manifestations in Sharp syndrome are reviewed.

Adult↗
PubMed1983

An in vitro study of hemicholinium-3 on phospholipid metabolism of Krebs II ascites cells.

With [Me-14C]choline as marker and after separation of choline and phosphocholine by ion-exchange column chromatography or thin layer chromatography on alumina, it is shown that 40 microM hemicholinium-3 (HC-3) inhibits the cytosolic choline-kinase of rat liver and Krebs cells. This inhibition is competitive (Km different, Vm similar) in the first case and mixed in the second (Km and Vm different). Despite this general inhibition of the phosphocholine formation, the synthesis of phosphatidylcholine (PC) by post-nuclear supernatants of rat liver and Krebs cells is different when tested with HC-3. It is unaffected in rat liver; however, HC-3 induces a PC deficiency in Krebs cells which is time-course dependent between 15 and 120 min and proportional to the drug concentrations in the interval 5-40 microM. Incorporation of AT-[gamma 32P] or [2-14C]ethanolamine into phospholipids shows that the sequential methylation pathway is not detectable in Krebs cells. These results are discussed in relation to those established concerning HC-3 action on phospholipid metabolism in other tissues.

Adenosine Triphosphate↗