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Biomedical subjects

M Hagihara

Publications and source records attributed to M Hagihara.

At least 109 records · Page 6Linked to original sources
PubMed1987

Dipeptidyl-aminopeptidase II in human cerebrospinal fluid: changes in patients with Parkinson's disease.

By using a sensitive and specific method, DAP II activity was found in CSF. DAP II activity in CSF of control patients without neurological diseases was 0.416 +/- 0.141 (mean +/- SD) nmole/min/ml and was higher than DAP IV activity in CSF, 0.221 +/- 0.062 (mean +/- SD) nmole/min/ml. In contrast, DAP II activity in serum was 1.16 +/- 0.16 (mean +/- SD) nmole/min/ml and was lower than serum DAP IV activity [41.85 +/- 3.36 (mean +/- SD) nmole/min/ml]. This relatively high activity of DAP II in CSF compared with the activity of DAP IV in CSF together with recent histochemical evidence on the localization of DAP II in some neurons (7) suggests that CSF DAP II may be derived from the brain and may be a marker of some peptidergic neurons. DAP II activity in CSF of patients with Parkinson's disease was significantly increased, whereas DAP IV activity in CSF did not change significantly.

Chromatography, High Pressure Liquid↗
PubMed1987

Post-proline cleaving enzyme in human cerebrospinal fluid from control patients and parkinsonian patients.

PPCE activity was found in human CSF by using a HPLC-fluorescence method. PPCE activity in CSF from control patients without neurological diseases was 2.19 +/- 0.78 (mean +/- SD) pmole (hr)-1.(ml)-1. PPCE activity in CSF from patients with Parkinson's disease was significantly decreased while PPCE activity in serum did not change significantly.

Chromatography, High Pressure Liquid↗
PubMed1987

Activities of dipeptidyl peptidase II and dipeptidyl peptidase IV in mice with lupus erythematosus-like syndrome and in patients with lupus erythematosus and rheumatoid arthritis.

We examined the activities of peptidases in plasma and tissues of the New Zealand Black (NZB) mouse as an animal model of human systemic lupus erythematosus, and also in serum from patients with rheumatoid arthritis and systemic lupus erythematosus. Activities of dipeptidyl peptidase II (DAP II) and post-proline cleaving enzyme (PPCE) were increased, and dipeptidyl peptidase IV (DAP IV) activity was decreased in plasma and spleen of NZB mice, as compared with the control BALB/c mice. Likewise, the activity of DAP II was increased and that of DAP IV was decreased in serum of patients with rheumatoid arthritis and systemic lupus erythematosus. These results indicate the importance of hydrolytic enzymes in the pathogenesis of autoimmune diseases.

Animals↗
PubMed1984

Serum lipid peroxide levels of patients suffering from thermal injury.

Lipid peroxide levels and activities of various enzymes were examined in sera from five thermally injured patients. In all patients examined, serum lipid peroxide levels were increased in the early post-burn period, and thereafter activities of glutamate oxalacetate transaminase, glutamate pyruvate transaminase, alkaline phosphatase and lactate dehydrogenase in the sera became elevated in most patients. From these observations, it is considered that increased lipid peroxides in the bloodstream during the early post-burn period would cause damage to various organs, permitting the leakage of the enzymes into the blood. These results support the view that lipid peroxide may be regarded as a 'burn toxin'.

Adult↗
PubMed1984

Age-dependent changes in lipid peroxide levels in the lipoprotein fractions of human serum.

Lipid peroxide levels in serum lipoprotein fractions of young and old individuals, who had no significant disease, were studied. The lipid peroxide level, amount of lipids, and the ratio of the former to the latter were significantly elevated in the low density lipoprotein fraction of older adults. This indicates that the elevation of the serum lipid peroxide levels in older adults is mainly due to increased lipid peroxide level in the low density lipoprotein fraction.

Adult↗
PubMed1981

Lipid peroxide levels and lipids content of serum lipoprotein fractions of pregnant subjects with or without pre-eclampsia.

Total serum lipids in normal pregnant subjects were significantly higher than those in non-pregnant subjects. This was due to an increase in the lipid content in all of the lipoprotein fractions, especially in VLDL. Serum lipids in pre-eclamptic subjects were higher than those in normal pregnant subjects. This was due to the increase in both VLDL and LDL. Serum lipid peroxide levels in pregnant subjects were significantly higher than those in non-pregnant subjects and further elevation of the levels was observed in pre-eclamptic subjects. The elevation of the peroxide levels in normal pregnant subjects was due to changes in all of the fractions of serum lipoproteins, especially in VLDL. The further elevation of peroxide in pre-eclamptic subjects resulted from an increase in HDL fraction.

Female↗
PubMed1976

Labelling of sarcoplasmic reticulum membranes with 1-dimethylaminonaphthalene-5-sulfonyl chloride.

1. Sarcoplasmic reticulum membranes were labelled with 1-dimethylaminonaphtalene-5-sulfonyl chloride (DnsCl). Analyses of the dansylated membranes demonstrated that the most of the dye was associated with ATPase (ATP phosphohydrolase, EC 3.6.1.3) and phosphatidylethanolamine in the membranes. 2. Dansylation of the membranes could be performed without significant decrease in the ATPase activity. 3. Partial differentiation of fluorescence of Dns-phosphatidylethanolamine from that of Dns-ATPase could be achieved by changing excitation wavelength; Dns-ATPase emmitted in the shorter wavelength region, while Dns-phosphatidylethanolamine emmitted in the longer wavelength region. 4. Fluorescence polarization of the dye bound to the membranes indicated that both the ATPase and phosphatidylethanolamine were strongly immobilized in the membranes, while the ratio of freely rotating dye to the "frozen" dye bound to the ATPase was larger than that bound to the phosphatide.

Adenosine Triphosphatases↗