Biomedical subjects
M H Hughes
Publications and source records attributed to M H Hughes.
Streptococcal sore throat.
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Gastroenteritis due to Salmonella subgenus 3 (Arizona). A second case diagnosed in Britain.
The second patient with Arizona gastroenteritis to be diagnosed in Britain had just returned from the United States, where his illness began.
Spoons and dip-slides.
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Bacteriological swabs.
Streptococcus pyogenes survives poorly on plain cotton-wool swabs, whereas serum-dipped swabs permit its survival but also allow overgrouth by other bacteria and are likely to contain virus inhibitors. The use of swabs dipped in a solution of 20% bovine albumin (fraction V) and sterilized by steam is therefore recommended, since these permit bacterial survival without growth and are unlikely to contain virus inhibitors.
Salmonella ponna in Hampshire.
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Infant diarrhoea due to Escherichia coli 091 K? H7.
A small outbreak of infective diarrhoea occurred among babies in hospital at Winchester, England. The causal agent was found to be a strain of Escherichia coli 091 K? H7 which was resistant to several antibiotics. Epidemic diarrhoea due to E. coli 091 has previously been reported from south Germany.
Pyruvic acid egg medium for tubercle bacilli.
Sputum culture for tubercle bacilli on a solid egg medium containing glycerol was compared with culture on a similar medium containing pyruvic acid and no glycerol. Tubercle bacilli from the sputum of five out of 99 patients grew on pyruvic acid medium but not on glycerol medium. The addition of pyruvic acid is therefore essential when an egg medium is used for the cultural diagnosis of tuberculosis. There was no clear relation between catalase activity or isoniazid resistance and preferential growth of tubercle bacilli on pyruvic acid medium.