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Biomedical subjects

M Grote

Publications and source records attributed to M Grote.

61 records · Page 4Linked to original sources
PubMed1978

Fine structural analysis of the morphological changes involved in the blanching, cooking, dehydration and rehydration of green bean pod tissue.

The influence of blanching and cooking on green bean pod tissue (Phaseolus vulgaris var.nanus L.) was studied by transmission electron microscopy and characteristic histological and cytological effects were recorded. The analysis of dehydraded and rehydrated samples (dehydration by hot air, rehydration by boiling) showed that much of the shrinkage after dehydration can be compensated by water absorption during rehydration. The morphological appearance of boiled raw tissue, however, is not reached.

Cooking↗
PubMed1978

Electron microscopic studies in cultivated plants. I. Green pods of Phaseolus vulgaris var. nanus L.

Scanning (SEM) and transmission (TEM) electron microscopic studies in the legumen of Phaseolus vulgaris var. nanus L. (kidney beans) were carried out. In this work emphasis was laid on the analysis of the morphological structure of the parenchyma tissue, being the chief component of the pod at edible maturity. The leaf character of the pod becomes especially evident by the occurence of stomata, trichomes, and typical cuticle structures on the outside of the pod (SEM). The cells of the mesophyll-like parenchyma tissue are distinguished by a great range of variation of their plastids (chloroplasts--chloroamyloplasts--amyloplasts) and some special cytological features such as a strongly developed rough ER, big nuclei, and numerous plasmodesmata (TEM).

Cell Nucleus↗
PubMed1978

Electron microscopic studies in cultivated plants. II. Fresh and stored roots of Daucus carota L.

Scanning (SEM) and transmission (TEM) electron microscopic studies in fresh and stored roots (4 months at an average temperature of +8 degrees C) of Daucus carota L. (carrots) were carried out. Chief stress was laid on the analysis of the histological and cytological structure of the secondary phloem parenchyma cell. SEM images show the three-dimensional histological arrangement of the cells. Moreover, the outlines of lipid droplets, mitochondria, starch grains, and carotin pigment crystals are visible within the parietal cytoplasmic layer. In TEM, besides the usual cell organelles (mitochondria, golgi-apparatus, ER, etc.) nuclei with several nuclear bodies can be recognised. The morphological structure of the chromoplasts shows a remarkable diversity. Within stored carrots a strong reduction of lipid droplets and starch grains within the chromoplasts takes place.

Carotenoids↗
PubMedDate not supplied

Humoral immune responses to recombinant tree pollen allergens (Bet v I and Bet v II) in mice: construction of a live oral allergy vaccine.

Recombinant tree pollen allergens (recombinant Bet v I and recombinant birch profilin, Bet v II) were purified and used to immunize BALB/c and B6D2F1 mice with Al(OH)3 to elicit a specific IgE response. Serum from immunized mice was then used to detect immunoblotted natural tree pollen allergens. The onset of the humoral immune response was monitored using antimouse IgE, IgG1, IgG2a/b, IgG3 and IgA. In both strains, a specific and long-lasting IgE response could be elicited with both recombinant allergens. Mice immunized continuously with recombinant Bet v I + Al(OH)3 showed a significant decrease of specific IgE antibodies indicating that continuous application of allergens can reduce specific IgE responses. The possibility of inducing a different type of immune responses is indicated by the fact that mice fed with Bet v I expressed in apathogenic Salmonella strains showed a Th1 immune response to Bet v I accompanied by specific IgG2a/b without detectable IgG1 or IgE. Recombinant allergens can hence be used to decrease or even modulate specific IgE responses in vivo.

Adjuvants, Immunologic↗