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Biomedical subjects

M Grey

Publications and source records attributed to M Grey.

15 recordsLinked to original sources
PubMed1992

A ten-minute protocol for transforming Saccharomyces cerevisiae by electroporation.

We present a simplified and rapid method for the transformation of yeast cells by electroporation. Stationary cells, scraped off the agar of Petri dish cultures stored in the refrigerator for up to 6 weeks, are suspended in sorbitol buffer, spun down by gentle centrifugation, transferred into the electroporation cuvette, and immediately subjected to transformation via electroporation. Transformation efficiency of this 10-min method, which does not require the preparation of cell cultures, is about 10% of the hitherto best performing transformation procedure using cells of defined growth phase.

DNA, Fungal↗
PubMed1992

Rapid and simple isolation of DNA from agarose gels.

We present a simple method for the isolation of DNA from agarose gels that is economic, fast, and independent of electrical equipment. DNA fragments of up to 6 kb can be easily extracted within 5 min using a disposable plastic syringe and filter paper. Total extraction of DNA fragments between 10 and 20 kb in size is achieved by concentrating the DNA flushed from the gel in a DNA-binding column.

Cloning, Molecular↗
PubMed1992

Children's participation in research: their right to consent.

This article discusses the ethical and legal considerations related to the inclusion of children in the research process. Specific attention is paid to situations in which customary guidelines are inappropriate. In addition, the results of a survey to determine the manner in which Institutional Review Boards are addressing these issues is presented with suggestions to assist nurse researchers in acquiring review board approvals.

Child↗
PubMed1991

Adaptation to chronic illness in childhood: diabetes mellitus.

The purpose of this paper is to discuss a conceptual frame of reference for the study of human responses to chronic illness in childhood using diabetes mellitus as a model case and to review the research findings on physiological and psychological adaptation. In addition, the implications of these studies for nursing practice are presented.

Adaptation, Physiological↗
PubMed1979

Impaired parathyroid response to induced hypocalcemia in thalassemia major.

Ethylene diamine tetra-acetic acid-induced hypocalcemia was used as provocative test of parathyroid reserve in eight normocalcemic patients with thalassemia major (age 8 to 26 years) and five young adult control subjects (age 22 to 35). In response to an intravenous infusion of disodium EDTA (50 mg/kg), serum immunoreactive parathyroid hormone rose by 1.97 +/- 1.93 (SD) microliterEq/ml in the patients, controls showing a rise of 10.6 +/- 3.6 microliterEq/ml (t = 5.46, P less than 0.001). There was no relationship between parathyroid response and total iron burden as measured by serum ferritin- or desferrioxamine-induced urinary iron excretion. Impairment of parathyroid reserve is common in transfused patients with thalassemia major and may serve as a marker of significant iron overload.

Adolescent↗
PubMedDate not supplied

1988 NAPNAP membership survey: characteristics of members' practice.

In 1988 the characteristics of the 2560 members of NAPNAP were determined by means of a mailed survey. Approximately 52% of the members returned usable questionnaires. Members were asked for information about their educational preparation, demographic characteristics, and professional employment status. Data about members' practice, how their practice has changed over time where possible, and implications for the future are reported.

Data Collection↗
PubMedDate not supplied

1988 NAPNAP membership survey. Part II: characteristics of the members.

In 1988, the 2560 members of NAPNAP were surveyed about their educational preparation, demographic characteristics, and professional employment status. Questions were included on members' opinions about issues that should be emphasized by NAPNAP in the future. Approximately 52% of the members returned usable questionnaires. In this second part of the survey results, we report the characteristics of members, how these characteristics have changed over time, and what the members believe NAPNAP's future priorities should be.

Adult↗
PubMedDate not supplied

Collaboration.

Explore the source record for details and available documents.

Clinical Nursing Research↗
PubMedDate not supplied

Coping and adaptation in children with diabetes.

The purpose of this study was to investigate the influence of age, coping behavior, and self-care on psychological, social, and physiologic adaptation in preadolescents and adolescents with diabetes. Children (N = 103) with insulin-dependent diabetes mellitus (IDDM) between 8 and 18 years of age and their parents participated in the study. Findings indicated that preadolescent children were significantly less depressed, less anxious, coped in more positive ways, had fewer adjustment problems, and were in better metabolic control than their adolescent counterparts. Age and secondary sexual development were related to psychosocial adaptation and metabolic control of the diabetes. Further, those who coped by avoiding their problems and who were more depressed were the most likely to have problems in both adjustment and metabolic control with 56% of the variance in metabolic control explained by the variables studied. These findings indicate that preadolescents and adolescents cope differently with a chronic illness, and that interventions should be designed to identify and help those with inappropriate coping styles.

Adaptation, Psychological↗