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Biomedical subjects

M Gresíková

Publications and source records attributed to M Gresíková.

At least 19 recordsLinked to original sources
PubMed1997

Biology of tick-borne encephalitis virus.

Tick-borne encephalitis (TBE) virus is an important human pathogen belonging to the genus Flavivirus within the family Flaviviridae. The genome of the TBE virus is a single-stranded RNA (ssRNA) molecule of positive polarity encoding all the viral proteins within a single open reading frame (ORF). TBE virus shares common physical and genetic characteristic of the flavivirus genus. Two subtypes of the TBE virus have been described: (1) European, endemic in many parts of Europe and transmitted by Ixodes ricinus ticks, and (2) Far Eastern (Russian spring summer encephalitis (RSSE) virus), endemic in Far East and transmitted by Ixodes persulcatus ticks.

Disease Reservoirs↗
PubMed1994

Serological evidence of Hantaan virus in Slovakia during 1989-1991.

Nephropathia epidemica was supposed to be an European form of haemorrhagic fever with renal syndrome (HFRS). However, in 1989-1991 we found serological evidence of infection with Hantaan virus in patients' sera from Slovakia. Higher titers of antibodies against Hantaan virus were detected in sera of 6 out of 75 patients with febrile disease, renal failure and clinical diagnosis of HFRS. The higher titers of antibodies against Puumala virus were detected in sera of 6 out of 75 patients. Serologic differentiation between Hantaan and Puumala infection in patients was based on the detection of higher antibody titers (4-fold or greater differences).

Adolescent↗
PubMed1992

Peroral immunization of adult white mice with the Skalica strain from the tick-borne encephalitis virus complex.

Adult white mice immunized perorally with the infectious Skalica strain from the tick-borne encephalitis (TBE) virus complex did not show any clinical symptoms of illness. 56% of experimental animals immunized with two doses of the Skalica virus (the titer of virus was 6 x 10(10) LD50) were protected against the challenge with the Hypr strain of TBE virus. All mice immunized with the Skalica virus and having haemagglutination-inhibiting antibodies higher than 1:80 survived the challenge with the given dose of virulent TBE virus. No differences in the immunogenicity and protectivity were observed in experimental animals infected with infectious Skalica virus by oesophageal probe, or by drinking virus-containing medium. A higher protective activity against the virulent Hypr virus was observed in adult white mice immunized subcutaneously with the Skalica virus.

Administration, Oral↗
PubMed1992

[Vaccination against tick-borne encephalitis in the Central Slovakia Region using a Soviet vaccine].

During the period between 1986 and 1989 in the region 1311 people working in forestry were vaccinated against tick-borne encephalitis by the inactivated Soviet vaccine. Only some of the people had prevaccination examinations only in some reactogenity of the vaccine was investigated as well as the persistence of humoral antibodies one and two years after the basic vaccination. These examinations in some of the vaccinated subjects were made in autumn 1986 and spring 1987. After two doses of vaccine in the haemagglutination-inhibition test (HIT) the seroconversion was 77% (of 182 evaluated) with a geometric mean of 37.9. One year after the primary vaccination in 53% of the examined subjects the titre of humoral antibodies dropped to negative values. Two years after the primary vaccination in the HIT antibodies were detected in 44% of 57 examined subjects. None of the immunized subjects contracted tick-borne encephalitis.

Antibodies, Viral↗
PubMed1991

The relationship between the flaviviruses Skalica and Langat as revealed by monoclonal antibodies, peptide mapping and RNA sequence analysis.

The flavivirus Skalica was isolated from a bank vole in Czechoslovakia in 1976. It can be serologically distinguished from prototype strains of tick-borne encephalitis (TBE) virus and has a decreased virulence for adult mice. We have further defined the relationship of Skalica virus to other members of the TBE serocomplex (TBE European and Far Eastern subtypes, Langat and louping ill virus) by using a panel of 22 monoclonal antibodies, peptide mapping and RNA sequence analyses. By these criteria Skalica virus proved to be distinct from TBE virus and to be very closely related to Langat virus, differing by only two bases among a total of 416 nucleotides compared. The sequence of 22% of the Langat genome was determined and the encoded amino acid sequences were derived. Comparison of these with the corresponding amino acid sequences of TBE virus revealed a similarity of 85%, as opposed to 93% similarity between the European and Far Eastern subtypes of TBE virus.

Amino Acid Sequence↗
PubMed1990

To the antigenic classification of some viruses from the tick-borne encephalitis complex by monoclonal antibodies.

Two monoclonal antibodies (MoAb) to the Skalica virus from the tick-borne encephalitis (TBE) complex were used to compare Karshi and Royal Farm viruses with the Russian spring-summer encephalitis, Central European encephalitis (Hypr and Kumlinge strains) Skalica, Langat and Powassan viruses. The first MoAb was prepared by fusion of P3NS1 cells with BALB/c mouse spleen cells, immunized with the Skalica virus; it was of IgM class and reacted in haemagglutination-inhibition (HI) test (MoAb type 1). The second MoAb was of IgG class and reacted in complement-fixation (CF) test (MoAb type 2). MoAb type 1 reacted in the HI test with Russian spring-summer encephalitis (RSSE), Central European encephalitis (CEE) virus strains, Skalica and Langat viruses. No reaction was observed with Powassan, Karshi, and Royal Farm viruses. MoAb type 2 reacted in the CF test with all members of tick-borne encephalitis complex except the Powassan, Karshi, and Royal Farm viruses.

Animals↗
PubMed1990

Comparison of skalica, Hypr, and Langat viruses by kinetic haemagglutination-inhibition test.

The Skalica virus has been compared with Hypr and Langat viruses by kinetic haemagglutination-inhibition (HI) test. Using Hypr antigen, differences were observed between Skalica, Hypr, and Langat viruses. By the use of Skalica antigen, a close relationship between Hypr and Skalica viruses was detected, however, it was possible to differentiate the Langat virus. When Langat antigen was tested, a close relationship among all the three viruses was found.

Animals↗
PubMed1990

Detection of antibodies to the western and the eastern type of haemorrhagic fever with renal syndrome in patient's sera from Slovakia.

We present serological evidence of infection with western and eastern type of haemorrhagic fever with renal syndrome (HFRS) virus in patient's sera from Slovakia by indirect immunofluorescence. Treatment of sera with 2-mercaptoethanol decreased levels of haemagglutination-inhibition (HI) antibodies to the eastern type of HFRS suggesting primary infection of the patients by this virus.

Antibodies, Viral↗
PubMed1989

[The incidence and clinical picture of hemorrhagic fever with renal syndrome in East Slovakia: the eastern and western type].

Hemorrhagic Fever with renal syndrome (HFRS) is endemic in East Slovakia. The paper presents the clinical picture of 4 serologically confirmed cases. Two of the 4 patients had serological evidence of recent Hantaan virus infection. Positive immunofluorescent reactions with Puumala virus (CG 18-20) were detected in the sera of the other two patients. The obtained results suggest the circulation of two serotypes of HFRS in Slovakia.

Adolescent↗
PubMed1989

[Isolation of tick-borne encephalitis viruses from the cervical spinal cord in meningoencephalitis].

In 1988 the organs of a male were examined post mortem for the presence of viruses in isolation experiments on white suckling mice. The virus isolated from the cervical spinal cord was identified as the virus of tick-borne encephalitis. Although death from tick-borne encephalitis is rather rare in Slovakia, the disease requires great attention with special focus on preventive measures.

Encephalitis Viruses, Tick-Borne↗
PubMed1988

Detection of the antigen and antibodies to the eastern subtype of haemorrhagic fever with renal syndrome virus in small rodents in Slovakia.

Direct enzyme-linked immunosorbent assay (ELISA) was used for the demonstration of haemorrhagic fever with renal syndrome (HFRS) virus antigen in lung tissue of small rodents trapped in Eastern and Western Slovakia. The eastern subtype of HFRS virus antigen was demonstrated in the lungs of Apodemus agrarius and of the western subtype in the lungs of Microtus arvalis. Antibodies to HFRS virus antigen have been detected in Apodemus species (A. agrarius and A. flavicollis) in higher titres to the Eastern subtype.

Animals↗
PubMed1987

Experimental double infection with Coxiella burnetii and tick-borne encephalitis virus in Dermacentor reticulatus ticks.

Experimental parenteral inoculation of Dermacentor reticulatus ticks with the rickettsia Coxiella burnetii (C.b.) and tick-borne encephalitis (TBE) virus resulted in a generalized rickettsial and viral infection, irrespective of whether the agents were given simultaneously or by a 7 days interval apart. Both agents multiplied intensively in ticks, C.b. being detectable predominantly in cytoplasmic vacuoles and TBE virus mostly in the endoplasmic reticulum.

Animals↗
PubMed1986

Serological survey with the antigen of haemorrhagic fever virus with renal syndrome in small rodents in Slovakia.

Results of serological survey in small rodents with the antigen of haemorrhagic fever with renal syndrome (HFRS) virus indicate the existence of a natural focus of this virus in Slovakia. Antibodies were found in the following free-living rodent species: Clethrionomys glareolus, Apodemus agrarius, Apodemus sylvaticus, Pitymys subterraneus, Microtus arvalis and Microtus economus. A total 10 out of 120 (8.2%) small rodents trapped in investigated localities of Western and Eastern Slovakia had antibodies to the HFRS virus antigen as detected by complement fixation (CF) and/or immunofluorescence (IF) tests.

Animals↗
PubMed1986

Preparation and characterization of hybridomas secreting monoclonal antibodies to tick-borne encephalitis virus.

Monoclonal antibodies (MA) were prepared to two strains of tick-borne encephalitis (TBE) virus: strain 4072 isolated from a patient in the U.S.S.R. and low-pathogenic for mice strain Skalica, isolated from a bank vole (Clethrionomys glareolus) in Slovakia. MA specific to the 4072 and Skalica strains were produced by hybridomas of the KEN (60 clones) and NEK (65 clones) series, respectively. Chromosomal analysis of MA producing 114 hybridoma clones of both series revealed a great variability in the number of chromosomes either in the range of given clones or between individual clones. The hybridoma cells under study possessed a high degree of transformation manifested by good growth in the mouse peritoneal cavity and marked accumulation in ascitic fluid (AF).

Animals↗